EMAGE mouse gene expression spatial database

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EMAGE:10804

Serpina1c serine (or cysteine) peptidase inhibitor, clade A, member 1C ( MGI:891969)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003228. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003228_01	euxassay_003228_02	euxassay_003228_03	euxassay_003228_04

euxassay_003228_05	euxassay_003228_06	euxassay_003228_07	euxassay_003228_08	euxassay_003228_09

euxassay_003228_10	euxassay_003228_11	euxassay_003228_12	euxassay_003228_13	euxassay_003228_14

euxassay_003228_15	euxassay_003228_16	euxassay_003228_17	euxassay_003228_18	euxassay_003228_19

euxassay_003228_20	euxassay_003228_21	euxassay_003228_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10804_wholemount_strong.wlz

10804_wholemount_moderate.wlz

10804_wholemount_weak.wlz

10804_wholemount_possible.wlz

10804_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10804_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 08 09 10 11 12 15 16

midgut

strong

regional

strong expression: see section 16 moderate expression: see section 17

liver lobe

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

kidney calyx

strong

regional

strong expression: see section 07 08 19 20

foregut-midgut junction lumen

strong

regional

strong expression: see section 11 12 13 15

rectum lumen

strong

regional

strong expression: see section 13 14

rest of hindgut lumen

strong

regional

strong expression: see section 13 14 15 16

midgut loop lumen

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4887
Entity Detected:	Serpina1c, serine (or cysteine) peptidase inhibitor, clade A, member 1C ( MGI:891969)
Sequence:	sense strand is shown >T4887 CGGACGCGTGGGCCTCGGTCACCACCCAGCTCTGGGACAGCAAGCTGAAAATGACTCCCTCCATCTCATG GGGTCTACTGCTTCTGGCAGGCCTGTGTTGCTTGGTCCCCAGCTTTCTGGCTGAGGATGTTCAGGAGACA GACACCTCCCAGAAGGATCAGTCCCCAGCCTCCCATGAGATCGCTACAAACCTGGGAGACTTTGCCATCA GCCTATACCGGGAGCTGGTCCATCAGTCCAACACTTCCAACATCTTCTTCTCCCCAGTGAGCATTGCCAC AGCCTTTGCTATGCTCTCCCTAGGGAGCAAGGGTGACACTCACACGCAGATCCTAGAGGGCCTGCAGTTC AACCTCACACAAACATCGGAGGCTGACATCCACAAGTCCTTCCAACACCTCCTCCAAACCCTCAACAGAC CAGACAGTGAGCTGCAGCTGAGCACAGGCAATGGCCTCTTTGTCAACAATGACCTGAAGCTGGTGGAGAA GTTTCTGGAAGAGGCCAAGAACCATTATCAGGCAGAAGTCTTCTCTGTCAACTTTGCAGAGTCAGAGGAG GCCAAGAAAGTGATTAATGATTTTGTGGAGAAGGGAACCCAAGGAAAGATAGTTGAGGCGGTGAAAAAAC TGGACCAAGACACAGTTTTCGCCCTGGCAAATTACATTCTCTTTAAAGGCAAATGGAAGAAGCCATTCGA TCCTGAGAACACTGAGGAAGCTGAGTTCCACGTGGACGAGTCCACCACGGTGAAGGTGCCCATGATGACC CTCTCGGGCATGCTTGATGTGCACCATTGCAGCACGCTGTCCAGCTGGGTGCTGCTGATGGATTATGCAG GCAATGCCACTGCTGTCTTCCTTCTGCCCGATGATGGGAAGATGCAGCATCTGGAGCAAACTCTCAGCAA GGAGCTCATCTCTAAGTTCCTGCTAAACAGGCGCAGAAGGTTAGCCCAGATCCACTTCCCCAGACTGTCC ATCTCTGGAGAATATAACTTGAAGACACTCATGAGTCCACTGGGCATCACCCGAATCTTCAACAATGGGG CTGACCTCTCCGGAATCACAGAGGAGAATGCTCCCCTGAAGCTCAGCCAGGCTGTGCATAAGGCTGTGCT GACCATCGATGAGACAGGAACAGAAGCTGCAGCAGTCACAGTCTTACTAGCCGTTCCTTATTCTATGCCC CCTATCCTGCGCTTCGACCACCCTTTCCTTTTCATAATATTTGAAGAACACACTCAGAGCCCCCTCTTTG TGGGAAAAGTGGTAGATCCCACACATAAATGACCACCCTAAGATGTCATCCTTCCTTCTGAATTGGGTTC CTTCCATTAAACACAGGCTGGCCTGGCTCGTGCCTGATGCTACAGCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5099206 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5099206 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10803 same embryo
	EMAGE:10802 same embryo
	EMAGE:10801 same embryo
	EMAGE:10805 same embryo
	EurExpress:euxassay_003228 same experiment
	MGI:4827976 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source