EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10820

Wdr89 WD repeat domain 89 ( MGI:1919588)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003115. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003115_01	euxassay_003115_02	euxassay_003115_03	euxassay_003115_04

euxassay_003115_05	euxassay_003115_06	euxassay_003115_07	euxassay_003115_08	euxassay_003115_09

euxassay_003115_10	euxassay_003115_11	euxassay_003115_12	euxassay_003115_13	euxassay_003115_14

euxassay_003115_15	euxassay_003115_16	euxassay_003115_17	euxassay_003115_18	euxassay_003115_19

euxassay_003115_20	euxassay_003115_21	euxassay_003115_22	euxassay_003115_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10820_wholemount_strong.wlz

10820_wholemount_moderate.wlz

10820_wholemount_weak.wlz

10820_wholemount_possible.wlz

10820_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10820_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4221
Entity Detected:	Wdr89, WD repeat domain 89 ( MGI:1919588)
Sequence:	sense strand is shown >T4221 CTTTTTTTTTAATTAATCAGGAGCTATATTTATACTATATTTACATTTGGTTAGCCAGCACCCAGGTACT TTGCTGATGAAAAAAGAAATTACCTTACTAGATTCATTTTGAGACTTAACATAAGACAGGCAAAAGAAAA TACAACAGCACGGCTATTTTTTTCAAAATGGCCTTTCAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC ACCCAACTGCTCTTCCGAAGGTCCAGAGTTCAAATCCCACAACCACATGGTGGCTCACAACCATCTGTAA CAAGATCTGATGCCCTCTTCTGGAGTGTCTGAAGACAGCGCTACAGTGTACTTACATATAATAAATAAAT CTTAAAAAAAAAAAAAAAAGAGCTTTTCGCCGCCGGACGCTGCCGAGGTCGCACGCGTGAGGTCTGTCCC CCGCAGCCGACATGCGCTACGTCGCCTCTTACCTGCTGGCCGCCCTCGGGGGCAACTCCTCTCCTAGCGC CAAAGACATCAAGAAAATACTAGGCAGCGTGGGCATCGAAGCGGACGATGATCGGCTCAACAAGGTCATC AGTGAGCTGAATGGAAAGAACATTGAGGATGTCATTGCTCAGGGTGTTGGCAAGCTGGCCAGTGTGCCTG CTGGTGGGGCTGTGGCTGTTTCTGCTGCCCCTGGCTCTGCAGCACCTGCTGCTGGTTCTGCCCCCGCTGC AGCAGAGGAGAAGAAAGATGAGAAGAAGGAGGAGTCCGAGGAGTCGGATGACGACATGGGATTTGGCTTG TTTGATTAAATTCCTGCTCCCCTGCAAATAAAGTCTTTTTATGTATCTTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4501749 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4501749 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10821 same assay
	EMAGE:10822 same embryo
	EMAGE:10819 same embryo
	EurExpress:euxassay_003115 same experiment
	EMAGE:10818 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source