EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10823

Mknk1 MAP kinase-interacting serine/threonine kinase 1 ( MGI:894316)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001217. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001217_01	euxassay_001217_02	euxassay_001217_03	euxassay_001217_04

euxassay_001217_05	euxassay_001217_06	euxassay_001217_07	euxassay_001217_08	euxassay_001217_09

euxassay_001217_10	euxassay_001217_11	euxassay_001217_12	euxassay_001217_13	euxassay_001217_14

euxassay_001217_15	euxassay_001217_16	euxassay_001217_17	euxassay_001217_18	euxassay_001217_19

euxassay_001217_20	euxassay_001217_21	euxassay_001217_22	euxassay_001217_23	euxassay_001217_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10823_wholemount_strong.wlz

10823_wholemount_moderate.wlz

10823_wholemount_weak.wlz

10823_wholemount_possible.wlz

10823_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10823_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 13 14 15 16 17 18

pancreas

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18

liver lobe

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 01

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4697
Entity Detected:	Mknk1, MAP kinase-interacting serine/threonine kinase 1 ( MGI:894316)
Sequence:	sense strand is shown >T4697 CCACGCGTCCGCGCGGCCGTTCCCGCGGTGGTCAACTAAGATCTCTGTGCTGGAGGAGATGAAGCAGAAG CAGGTGGCAGACTAAAGGACACTGGCTGGACAGAGACCAGCAGATAACTCCCTGTGGCTGGAAGACACCA GTCTTGGGATTCCTCTGAGACTCCAAGTTAAAGAACCTTGTGGAGATGGGCAGCAGTGAGCCCCTTCCCA TCGTGGATTCTGACAAGAGGAGGAAGAAGAAGCGTAAGACCCGGGCCACCGACTCTCTGCCAGGAAAGTT TGAAGATGTGTACCAGCTGACCTCGGAATTGCTGGGAGAAGGAGCCTATGCCAAAGTCCAGGGTGCTGTG AACCTACAGAGTGGGAAGGAGTATGCTGTCAAAATCATCGAGAAGCAAGCCGGGCACAGTCGAAGTCGAG TGTTCCGTGAGGTGGAGACACTGTATCAGTGTCAAGGGAACAGGAACATTTTGGAGCTGATTGAATTCTT TGAAGATGACACACGGTTTTACTTGGTCTTTGAGAAATTGCAAGGAGGCTCCATCCTAGCACACATCCAG AAGCGGAAGCACTTCAATGAGCGAGAAGCCAGTAGAGTGGTGCGGGACGTCGCCACTGCCCTTGACTTCC TGCACACTAAAGGCATTGCTCACCGTGATCTGAAGCCAGAAAACATACTGTGTGAATCTCCAGAAAAGGT GTCTCCGGTGAAAATTTGTGACTTTGACTTGGGCAGTGGGGTAAAGTTGAACAACTCCTGCACTCCCATA ACCACGCCAGAGCTGACTACTCCATGCGGCTCTGCAGAGTATATGGCACCTGAGGTGGTGGAGGTCTTTA GGGACGAGGCTACTTTCTATGACAAGCGCTGTGACCTGTGGAGCCTGGGTGTGGTCCTCTACATCATGCT GAGTGGCTACCCCCCCTTTGTAGGTCACTGTGGGGCTGACTGTGGCTGGGACCGGGGAGAGGTATGCAGG ATGTGCCAGAACAAGCTGTTTGAGAGCATCCAGGAAGGCAAATACGAGTTTCCTGACAAAGACTGGGCTC ACATCTCCAACGAGGCCAAAGACCTCATCTCTAAGCTCCTGGTTCGAGATGCGAAGCAAAGACTCAGTGC TGCCCAGGTTCTGCAGCATCCATGGGTACAAGGGCAAGCTCCAGAAAGGGGACTCCCCACGCCGCAAGTC CTTCAGAGAAACAGCAGCACCATGGACTTGACTCTCTTCGCAGCTGAGGCCATTGCCCTGAACCGCCAGC TGTCTCAGCATGAGGAGAATGAACTGGCCGAGGAACAGGAGGCCCTAGCTGAGGGCCTCTGCTCCATGAA GCTGTCCCCTCCATCCAAATCTCGCCTGGCTCGAAGGCGAGCCCTGGCCCAGGCTGGCCGAAGCCGAGAT GCAAACCCATGTTTGACACCGGCAGGGCTCTGAACCACTGCAGGCACTCCTTGTAGGCCTGTCCCAGCGG TGCCCCCTGAAAACCTGTAAGGTCAAAGTGTGAAGTAGGCAGAGGGACCTGCCCTGCAGCCCCTGCAGGC CTTTACCATCCACAAAGGCTCCCGGGTTCCCACCCATCCCCCATTTCCCTGGAGTCTTCAAAGGAGAAAG CTTTTTCCAAAGGGGTTGTCTTTGAAAAGGAAAGCAATCACTTGTCACTTTGCATAATCGCCTGCAGCAG GACCGTCTCTGCCGGGCTCCAAAGGCTGCTGATGTGCAGATCCCGGATCCAGCTTGCGGTTGGGGCTGCA GGGGCTGCAGCCTTCAGGGAGTCCAGCTTCCAGGAGCTGACAGATCTGCGGACGTGCATGCCTCTTCCCT GCACTGTCACCTCCTTCTGGCGATCCTCCCACCTTCCTCTGTCCTACAGACGTCCTCTCTGCCTGTCCTC TCCCTGGCTGAGCAAAGCCATCCCCTCAATTCAGGAAGGGCAGGGAACCAGATCAGTCTTCCGGTCTGTA ACACAGAGAAGCACATAATCTTTCTTTGCCGTGACCGAAATGTTCCTCATTTATCATCCTCTTCCATGTT TGGGATTGCTGCTAAAGTCAGTATTATTTCTTTTTAAAAATATATTTGCTTATTTAAACCATGTTCTTCC AGCAATCATAGAACTTCAAACTTGCACTGCAAGCCCGTAGATATTCCCCACGCGGATATCCACGCACTTA GGTTTTAATCCTCTCCCCGACAGTCTCAACTGATTTTAAGCTGTTACTATGATGAAAACGTTAGAGACTG GTGTCTTTGCATCTCTGTCTGCCGCTGCTGCCTCCACAAACCCAGGGTAGTCAGACTGCTGTGTCTGAGA GGAAGCCCTGTGCCCTGCTATGAGGAATGCTGCGCCCTCTGCCCCAGACATGAAGGCACAGCCACTGGAA AATAAGCTGTGGAGTCACGTAAAACAGATTTGCATATATGACATACAACATTAGTCAACCACAATCACCA AAATTCTCTCATAACTTGTGCTTGTTTTAATAAATAATTTAAAATTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5030044 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5030044 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10826 same embryo
	EMAGE:10824 same embryo
	EMAGE:10825 same embryo
	EMAGE:10827 same embryo
	EurExpress:euxassay_001217 same experiment
	MGI:4826387 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source