EMAGE mouse gene expression spatial database

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EMAGE:10825

Tcta T-cell leukemia translocation altered gene ( MGI:1918829)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006556. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006556_01	euxassay_006556_02	euxassay_006556_03	euxassay_006556_04

euxassay_006556_05	euxassay_006556_06	euxassay_006556_07	euxassay_006556_08	euxassay_006556_09

euxassay_006556_10	euxassay_006556_11	euxassay_006556_13	euxassay_006556_14	euxassay_006556_15

euxassay_006556_16	euxassay_006556_17	euxassay_006556_18	euxassay_006556_19	euxassay_006556_20

euxassay_006556_21	euxassay_006556_22	euxassay_006556_23	euxassay_006556_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10825_wholemount_strong.wlz

10825_wholemount_moderate.wlz

10825_wholemount_weak.wlz

10825_wholemount_possible.wlz

10825_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10825_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

homogeneous

moderate expression: see section 06 07 08 09 22 23 24

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 09 10 21 22

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 19 20 21 22 23 24

vagus x ganglion

strong

homogeneous

strong expression: see section 10 11 20 21

vestibulocochlear viii ganglion

moderate

homogeneous

moderate expression: see section 09 10 22

trigeminal v nerve

moderate

regional

moderate expression: see section 11 19

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 13 18 19 20 21 22

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6749
Entity Detected:	Tcta, T-cell leukemia translocation altered gene ( MGI:1918829)
Sequence:	sense strand is shown >T6749 GCCAGTCATGGCGGAGCCTTGGGCCGGGCAGTTTCTGCAAGCTTTGCCCGCCACGGTGCTCGGAGCGCTG GGCACCCTGGGCAGCGACTTTCTGCGGGAGTGGGAGACACAAGACATGCGAGTGACTCTCTTCAAGCTGC TCCTGCTGTGGTTGGTGTTAAGTCTCCTGGGCATCCAGCTGGCCTGGGGGTTCTACGGGAACACAGTGAC CGGGTTGTATCACCGTCCAGATCCCCACCCTCAGCCTCCTGCAGCTATGGGTGTGTTCCTGCCTCCAGGT CTGGGTGGCCAGAATGGATCCACACCTGATGGCTCCACACATTTCTCTTCATGGGAGATAGCAGCAAATG AAGCTCTCAAAACGCACAGAGAATAAGGGAAGGCAGCAGCAGAAGGGTCTCCAGAGATATCGCTGGGTCC GCTGGCTCCTATGCTATTTGTTGCTGCTGTCCAGACCCCTTGGACAACTGCAGAGGGCTTGGGGTTGGGG GACTGGAGTACCATGATGCTTCCAGGGTGAGCCTCCACTGCCCTCAGCCTTGCCTCCAACAGCTTCAGGT ACTGGGCAGGTGAAGTTTGTCTCTTAACAAAAGGAAAGACTTCTTGTCAAGGGTCATTGCTTAGAAACAT TTTGGTTGAGGAGGTCAGTGGTCCCTTTCTCACCTTGGCTTTTTCCTGACTTAGTCATCCCCTTGGGGAT GTAATCTTAGAGAAATTGGTCCTTCTAAGAGGAATCTCGAGGTTGGCCAGTTCTGAGATTTAGAAAGAAA GCCAGTTCTCCCATAGACTCTGACTTTCTTGGCTTTGCTTCGTAGGCAAAAAGCCAGCTAATCCTGGCCT GGATTAGGGCACCAAACCTGTCCCTCCCTGGCCAAGCTACAATCTTTTAGGTCTGAGGCAGGCAGAGGAA GTTATCATTTCCCACTGACTTGTTTCTCTGTTCTTTTTGAGGACAAGTTGAATTCCTAAGAGACATCTCA CTAAGAGCCTTGGTATTGGCTAGCTCTCCACTGCTGAAGTACCCACAGGCTTTTCTAGTCTGTACTTGCT CCCTCCTAGGGAGGTACTAAGTAGTTAGTGCAATGACTTCACTGTGCCCTGTTACCTTGTCACTTCCCAT CTTGACAAGGGCCTAGTCCTGAGAGTCCACCAGCCTCATTTCAGCCTCTAGGAATAGCAGTAAGAGCAAG CCACAAGCCAGATAGCTCTGGGCCCTTCACCATGGGTCAGAAGAGGTTCCAAGGTGGTGGGTAGCCAGTG TCCCCATGAAAACACCACTTCCTCAGAGCACTGTGGTTCCTAAAAGCATTGTGGTCCTGGATCTATCTCC TCTAACAGTTGTACTCCTGTCTCTGCTGGGCTACTAGCTACCTCAGGTGTCCACTGTCTCTTTGTTAGAT GTCATATAGCCATCTGGCAGCCATTGCCTACTGAGACTTTTAGCAGGTGTACTATAGGTGAACTGTACTG AGCGCCTCGTGCACGTGCACCTACTCCAGAGATGTCATCTGTGTGGCAAGATGTTCTTCACAGCCTCATG CCCACCCACTGACATCACTACAAGAGTCAAGGTCTCACATTCCACCCCAGGAGCAACTGAAGATGTGGCA TATTAAACACAGAAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3672301 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3672301 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10826 same embryo
	EMAGE:10823 same embryo
	EMAGE:10824 same embryo
	EMAGE:10827 same embryo
	EurExpress:euxassay_006556 same experiment
	MGI:4828648 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source