EMAGE mouse gene expression spatial database

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EMAGE:10868

5430435G22Rik RIKEN cDNA 5430435G22 gene ( MGI:2442295)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003357. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003357_01	euxassay_003357_02	euxassay_003357_03	euxassay_003357_04

euxassay_003357_05	euxassay_003357_06	euxassay_003357_07	euxassay_003357_08	euxassay_003357_09

euxassay_003357_10	euxassay_003357_11	euxassay_003357_12	euxassay_003357_13	euxassay_003357_14

euxassay_003357_15	euxassay_003357_16	euxassay_003357_17	euxassay_003357_18	euxassay_003357_19

euxassay_003357_20	euxassay_003357_21	euxassay_003357_22	euxassay_003357_23	euxassay_003357_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10868_wholemount_strong.wlz

10868_wholemount_moderate.wlz

10868_wholemount_weak.wlz

10868_wholemount_possible.wlz

10868_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10868_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6746
Entity Detected:	5430435G22Rik, RIKEN cDNA 5430435G22 gene ( MGI:2442295)
Sequence:	sense strand is shown >T6746 CTCAGAGGGAAGTGGCCTCTCACCAAAACCGTTTGCGTTCTCAATACTGGCCAGATTGAAACTGCCACTC CTAGAAGAGAGACCCAGACCTAGAACTCCCACCTGTGTGAGGCCCTGAAGCCCCTCATGAATCCCCGAAA GAAAGTGGACTTGAAACTTATCATTGTTGGTGCACTTGGCGTGGGAAAGACCTCTCTTCTTCACCAATAT GTACACAAAACATTTTTCGAGGAATACCAGACCACACTGGGGGCCAGCATCCTCTCCAAAATCATCATAT TGGATGACACAACTTTGAAGCTGCAGATCTGGGACACAGGTGGTCAGGAGCGGTTCCGCTCAATGGTATC AACATTCTACAAAGGTTCCGATGGCTGTATCCTGGCATTTGATGTTACTGATCCAGAGTCCTTTGAAGCC CTGGATATCTGGAGGGATGATGTCCTGGCAAAGATTATCCCTATGGAGCAGTCATATCCCATGGTGGTGC TGGGGAACAAAATCGATCTGGAAGATAGGAAGGTGTCCCAGGAGGTGGTCCATGGATGGTGTAAAGAGAA GGATATGCCATATTTTGAAGTTAGTGCGAAGAATGACATCAATGTGGTACAAGCCTTTGAGGTTCTGGCC AGCCGGGCTCTGTTGAGGTACCAGGGCACCGCAGAGAACCACCTCATAGACTCCATCAAGCTCTCTCCAG GCCAGCCAAAGAGCAGATGCTGCTGATCCTGGCCTCCCTCCGGACTCCCGATGACAGCTGTGCAGCCATT CCAAGTGCAATCCCACCAGCCGGTACTCAGCTAGGAGGCTGGGGCACTGCAGCAGGAGAGAATGTCACAG GCAAATCCTGGTCCCAGAGAAATCACGGGCGTCTCCATGACTCAAGCTCCTCTTTCTCCTCTGCCTCAAC ACCGTCCTGCTCATCTCTGCAGACAAACTGCTCCCTTCAGCACCATGGACCGACAGGTTTGGATGGAGAT GCAGTTCTGATTCCCTCAGCAGCCAGAACATGGACCGCTCCTGACAGGCAGAAGCCCACAGGCCTGAGAC TTCCCACAGCTTCTTTCAGGGAGAGCTGAGGGAGCAAGATAAAAACAGTTGTACCTATTTCCTGGGTGCT CAAACAGCTCCCATAACTGACCAGGCCTCAGTGCGCCCTCTGCCAAATGAGAGTTCCCAAGGCTTCCCCA TATCCTAGGGCGACTGAGAACCATAATCTCATTTAAATCCCACGTGCTGGTGTGGACACACATGCTCACT CCTTTGGAGAGTATTTTTGATGCCTTCGATGTACATTGCACTGAAGTCACCAGACACACAAAGCAGACAT GAGTCCTTCACTCCCAGAAGCGCACCTGGAGAGAGAGGGCCAGCAGACATGAGCAAGACAGACAACAGCA ACGAGGTACTGTTTTAGACAAAGGGGCCAAGAGGGCTTTCCTGTGACGGTGACCGTTGGGCTGGCAGCTC CAGGAAGAGCTACAGGAAGAACATGCCAGGAGAGGGAGCAGCTAGGCCAAGGCTCTGAGGCAATGGGCAG AGTGCTGCATTCCATTCCCTGAGGACTCAAAGCTGCTCAGAGAAAAAAAGGCCAGGGATGTCCTGTCCCC AGAAGGTACTTTGATCATTTGATGACTGGCCACTAGGGGGCAGAAGTGTCCTCTGCTTTTAGGCTGAGGA GTTCTGGTAACACTTGAAAAGCTGGTGTTTGTGTCAAGAAAATGGCTGAAGCTTGTTCCCAGGCTGTAGG AATGTTGAACCTGGGCTAGTTGGGTTAAAAAAAAAAAATCAAATGTCTTGATTCAGGCAACAAATCGAAA TCAGAGACAGATCCCAGAGCGAGGTACTTGGAGAGCTGTGGATTCTGTGTCCTGTTCATTTCAAAGCTAG AACCTATTTTTTTTTCTTCTGGCTTCACACTGCACAGATAGGGTGGCTCATTCTGAAGGAGGTGGACAGA ACGAACTTTCAGGGTCTTCAATGGTCAGGTTCATACCTCATGCCTTCCACAGACATTCCTGTCTCCACAG AAGTCTTCTTTCCTGAGTTCCAAGCAGATCCGAGGTGCCAATCTCTTACACGCATTCTCACGACCGGCCA GGAAGAACGCAGCAAACCAGAATCTTCTGCGGCAAAACTTTATTGCTTACATCTTCAGGAGCAAGAGTGC AAGAGAGCAAGAGCTAGAAAGCAAGAAAAAGAACAAGAACAAGAAAGCAAGAGCAAGAGAGAGAAAAAGC AAGAACAAGAGAGAGAGAAAGAATGGCAAAACCCCGTCCCTTTTAAGGAGAATTATCCTCCGCCTAGGAC GTATTACTCCCTGATTGGCTGCAGCCCATCGGCTCAGTTGTCATCACGAGAAAGGCAGAACACATGGCGG GAAAACTGCCCCTGCACGTGTGCAGATTATGTTTACTACTTAGAACACAGCTGTCAGCGCCATCTTGTAA TGGCAAATGTGAGGGCGGCTCCCAACATCAATCTCTAGGAAGCCAGTCAGGGGGTACTAACTGGGGGTCT GGGGGCTCTGTCTGCACCCGCCTAGCTCTGAGGTATAAAGCAACTTACTAAGGACAGCAGTCACAGGAAC AGTGAGCCCTGCGTCCTTCAGAGATGAACACTGTGCCCCCGTCACACATTGGTGTGGCTCTCCTGAAGTC AGACTGTGGAGGGATGTGTGTGTGTGTGGGGGTGCATTCGGCAGCACAATGAACATAAGGACCATATCTA AGGGTACGCAGCAAATAGTATGTGTTTCCATTGTTTATATCCTTTATGGGACAATAGTCCCTCTTGGTCA GTGATACCAGTCTCAGTTACCCATGGTCAACTGTGGACCAAAAATATTAAATAGCAAATCCCAGAAATAA ATAACTTACAACTTTCAATGCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3672128 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3672128 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10867 same embryo
	EMAGE:10865 same embryo
	EMAGE:10866 same embryo
	EMAGE:10864 same embryo
	EurExpress:euxassay_003357 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source