EMAGE mouse gene expression spatial database

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EMAGE:10879

Hgs HGF-regulated tyrosine kinase substrate ( MGI:104681)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003310. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003310_01	euxassay_003310_02	euxassay_003310_03	euxassay_003310_04

euxassay_003310_05	euxassay_003310_06	euxassay_003310_07	euxassay_003310_08	euxassay_003310_09

euxassay_003310_10	euxassay_003310_11	euxassay_003310_12	euxassay_003310_13	euxassay_003310_14

euxassay_003310_15	euxassay_003310_16	euxassay_003310_17	euxassay_003310_18	euxassay_003310_19

euxassay_003310_20	euxassay_003310_21	euxassay_003310_22	euxassay_003310_23	euxassay_003310_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10879_wholemount_strong.wlz

10879_wholemount_moderate.wlz

10879_wholemount_weak.wlz

10879_wholemount_possible.wlz

10879_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10879_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate

moderate

regional

moderate expression: see section 17 weak expression: see section 08 09 16

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 08 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 16 17 18 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 14 17 weak expression: see section 10 11 12 13 16 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6185
Entity Detected:	Hgs, HGF-regulated tyrosine kinase substrate ( MGI:104681)
Sequence:	sense strand is shown >T6185 GGAGCGACTGCAGCGTCGGTCCGGAGTGGGGTCGCCATGGGGCGAGGCAGCGGCACCTTCGAGCGTCTCC TAGACAAAGCCACCAGCCAGCTTCTGTTGGAGTCAGACTGGGAGTCCATTCTACAGATCTGCGACCTGAT CCGTCAGGGGGACACACAAGCAAAATATGCTGTAAACTCCATCAAGAAGAAGGTTAATGATAAGAACCCA CACGTGGCTTTGTATGCTCTGGAGGTGATGGAGTCTGTGGTAAAGAACTGTGGCCAGACAGTCCATGATG AAGTGGCCAACAAACAGACCATGGAAGAACTGAAGGAGCTGCTGAAGAGGCAAGTGGAAGTTAATGTTCG GAACAAGATCTTGTACCTGATCCAGGCCTGGGCACATGCCTTCCGGAACGAACCCAAGTACAAGGTGGTC CAGGACACATACCAGATCATGAAGGTAGAAGGACATGTCTTCCCTGAATTTAAGGAAAGTGATGCCATGT TCGCTGCTGAAAGAGCCCCTGACTGGGTGGATGCTGAGGAATGCCATCGGTGCAGAGTACAGTTTGGGGT GGTGACCCGCAAGCATCACTGCCGAGCATGTGGGCAGATCTTCTGTGGCAAGTGCTCCTCCAAGTACTCC ACCATCCCCAAGTTCGGCATTGAGAAGGAGGTGCGCGTGTGTGAGCCCTGCTATGAGCAGCTGAACAAGA AGGCAGAAGGGAAGGCTTCCTCTACCACTGAGCTGCCCCCAGAGTACCTGACCAGCCCCCTGTCACAGCA GTCTCAGCTGCCCCCAAAGCGGGATGAGACAGCCCTGCAGGAAGAGGAGGAGCTACAGCTGGCTCTGGCC CTATCACAGTCAGAGGCTGAGGAGAAAGAGAGGATGAGACAGAAAACAACATATACAGCACATCCAAAGG CAGAGCCCACGCCCTTGGCTTCCTCTGCGCCCCCAGCTGGCAGCCTGTATTCCTCGCCTGTGAACTCATC AGCACCTCTGGCTGAGGACATCGACCCTGAGCTTGCAAGATACCTCAACCGGAACTACTGGGAGAAGAAA CAGGAGGAAGCACGGAAGAGCCCCACACCATCTGCACCTGTGCCCCTGACAGAGCCTGCTGCCCAGCCTG GAGAAGGACATACAGCCCCCAACAGCATGGCAGAGGCTCCTCTTCCAGAGACAGACTCTCAGCCCATAAC TCCCTGCAGCGGCCCCTTTAGTGAGTACCAGAATGGGGAGTCGGAGGAGAGCCATGAGCAGTTCCTCAAG GCCCTGCAGAATGCCGTGAGCACTTTTGTCAACCGCATGAAGAGCAACCACATGCGGGGACGCAGCATCA CCAACGACTCGGCTGTGCTGTCACTCTTCCAGTCCATCAACACCATGCACCCGCAGCTGCTCGAGCTGCT CAACCAGCTGGATGAGCGCAGGCTGTACTACGAGGGCCTTCAGGACAAGCTGGCACAGATACGTGACGCC CGAGGGGCTCTGAGCGCCCTGCGTGAAGAACACAGGGAGAAGCTGCGCCGGGCAGCTGAGGAGGCTGAGC GTCAACGCCAGATCCAGCTGGCACAGAAACTGGAGATCATGAGACAGAAGAAGCAGGAGTACCTGGAGGT GCAGAGACAGCTAGCTATCCAGCGCCTGCAGGAACAGGAGAAGGAACGGCAGATGCGTCTGGAGCAACAG AAGCAGACTGTCCAGATGCGCGCCCAGATGCCTGCCTTTCCCTTGCCTTATGCCCAGCTCCAGGCTATGC CCACGGCTGGGGGTGTACTCTACCAGCCCTCAGGCCCAACCAGCTTCCCTGCCACCTTCAGCCCAGCAGG CTCAGTAGAGGGCTCTCCGATGCATGGTGTGTATATGAGCCAGCCAGCCCCAGCCACTGGCCCCTACCCC AGCATGCCTGGCACAACAGCAGATCCCAGCATGGTCAGCGCCTACATGTACCCAACAGGTGCCCCTGGGG CACAGGCAGCCCCTCAGGCCCAGGCCGGGCCCACCACCAGTCCTGCCTACTCCTCCTACCAGCCCACACC AACCCCAGGCTACCAGAGCGTGGCTTCTCAGGCCCCACAGAGCCTCCCAGCCATCTCACAGCCTCCACAG ACCAGCAACATAGGCTACATGGGGAGCCAGCCAATGTCCATGGGCTACCAGCCGTACAATATGCAGAATC TCATGACCGCCCTTCCCGGGCAGGATGCGTCTCTGCCAGCCCAGCAGCCCTACATCCCAGGGCAGCAGCC CCTGTACCAGCAGATGGCCCCCAGCACCGGCCCTCCCCAGCAGCAACCCCCTGTGGCCCAGCCAGCGCCT ACACAGGGACCGCCAGCACAGGGCAGTGAGGCCCAGCTCATCTCCTTCGACTGACCTTGAGTCTGGCGCT CACCATGCAGAGTAACACTACAGTTCACCAGAAACCACTTATATGTCTAACTAGCCATTCCTCCCCTATT ACTGCCCTGTAGTGTCCCTTCTGTGAGCAAGGGGTGGGCCTTCCCCCTTGGCCCTCTTCCCTGTCCTCGG TGGTCTGGCTCCTATCACTGGTTCCCTGCTTCAGTCCTGATGCCGTCTGACCTCCCCGGGACTGGGGCTC TGCAGGGCAAGTAGAACCTTCTCTGGAGAGTGCCCGGCTGTGTTGGGTGCCACAGGTGAATGCTGTGGGG ATGCTTAGCCTGGCCCGGAGTCTGATGTGAACTAACTGTGTGGTGTCCAGGCCTGGCCCTGGTACCCAGA AGAACCATGTGGCTCCTGCACAGCAAGGGTAGAAGCTTTAGGTATCTCTGTCTCCCCTTCTCCTTGGATG TCATCTCTCAGTGCAGAGCAGTGAAAATAAAATGTATTTCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3497966 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3497966 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10882 same embryo
	EMAGE:10880 same embryo
	EMAGE:10881 same embryo
	EurExpress:euxassay_003310 same experiment
	MGI:4825369 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source