EMAGE mouse gene expression spatial database

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EMAGE:10881

Mrpl15 mitochondrial ribosomal protein L15 ( MGI:1351639)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003344. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003344_01	euxassay_003344_02	euxassay_003344_03	euxassay_003344_04

euxassay_003344_05	euxassay_003344_06	euxassay_003344_07	euxassay_003344_08	euxassay_003344_09

euxassay_003344_10	euxassay_003344_11	euxassay_003344_12	euxassay_003344_13	euxassay_003344_14

euxassay_003344_15	euxassay_003344_16	euxassay_003344_17	euxassay_003344_18	euxassay_003344_19

euxassay_003344_20	euxassay_003344_21	euxassay_003344_22	euxassay_003344_23	euxassay_003344_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10881_wholemount_strong.wlz

10881_wholemount_moderate.wlz

10881_wholemount_weak.wlz

10881_wholemount_possible.wlz

10881_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10881_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

weak

regional

weak expression: see section 03 04 05 06 07 21 22 23

glossopharyngeal ix ganglion

weak

single cell

weak expression: see section 06 07 08 18 19

trigeminal v ganglion

moderate

single cell

moderate expression: see section 04 05 20 21 22 23 weak expression: see section 03 06 07 08 09 18 19

vestibulocochlear viii ganglion

weak

single cell

weak expression: see section 06 07 08 18 19

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 12 16 17 18 weak expression: see section 10 11

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 weak expression: see section 05 06 07 08 21 22 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6190
Entity Detected:	Mrpl15, mitochondrial ribosomal protein L15 ( MGI:1351639)
Sequence:	sense strand is shown >T6190 CCACGCGTCCGCGCTTCCAGGGCGCCCTTGAAAAGACCGTGGGTCATGGCTGGCACGGCGCGCGGCTGCG GGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTC CAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAA AGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAA AATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTA TCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGT GTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAG CAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGT TGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTTGATGCACTCTTCTGACTTCCATGGATTT TCGGCAAGATACTCAATACACATAAAATAATAAAAAAGCCATTGAGAAAAGACATCTGACCAGCATCTGG CCTCCATGTGTGCCTGCATCTGGTCTCCATGTGTGCCTGCATCTGGCCTCCATGTGTGCCTGCATCTGCC CCCACATCCCATTTCTAAAGTAAGAGGGAAACTTTTGTTATGATCTGAGTCCCTGGTCTTTTTTTTTAAT TGTAAGTCTTTACAATTTTCTTTGGGAAGTAGAAGGTCCTTCTTAGGGTGGGGAGTGTCAGACGGGACCA GTACTCGTTTCACTTAGGGTGGAGGAGAGAGCTCTGATGTTAGTGTGAGTCCCATGACGGTTGTCTTTCG TGATAACTTGCTGTAGGCAGACCACTACACTCTAGCTTGCTCGTTACATGCTTGGAGCTGCACAGCCATC TGGGTGAAACACTTGAGTAGGACTTTAAGCAAGACTAGATTTGGGACTTTGGCATGCAATTTATTCTCTG CCTCAGTTTCACCATCTGCCAAATGGAGGGTAAAATAAAAATTTGGTTATTATAAAATACAAATTCTCTT AGGGATTTTACTGCTATAACCAGCACCATGACCAAAAGCCAGTTGGGAAGGAAGATTATTTTGCTTACAC GTCCGTAACACTGTTCATCAAGGAAGGAAGCCAGGATAAGAACTCAAACAGGGCAGCAGCTCATGTAGAG ACCATGGAGCATGCTGCTTACTGACTTGCTCCTCATGGCTTGCTCAGCCTGCTTCCTTATAAAACCCGAG ATCACCAGCCCACAGATGGCACCACCCACAATGGGCTGGGTCCTTCCCCATTAATCATTAAGAAAATGCC CTACAGGTTTGCCTACAGCTGGATTGTACAGAGGCATTTTCTTAATTGAGATTCCTTCATTTCAGATGAC TGTCAAGTTGACATAAAATTAGAACAGTTATTATGCAAGTCGCTAGTGTTTGTAAAGCATGTAGCACATG CCTGTTTCGTAGGCCTCTAGAGTTAGTGTCTCTTCTGATCCTTAGGTAACTGCCACCCTGTGGATTCTAC TTCCTTCCCTCTTAGGATTTACATATAGTCTGTAAAGCACTCTTGAGTCTCACTAATGAGAAGAAAATGC ATGATAGACTTTATATTGTTTTTAGCAGGAGCTAAAAAATACATTTTGAAGAAGCAGCATAGCAGAGGCA GAAACATAGCACCCAAGTCTGAAACAATTGGCACCAGTCTTCCCTTCTGCAGTTTGTTCAATAAACAGAA TTCCTAAATCTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498041 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498041 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10879 same embryo
	EMAGE:10882 same embryo
	EMAGE:10880 same embryo
	EurExpress:euxassay_003344 same experiment
	MGI:4826441 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source