EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10894

Cx3cl1 chemokine (C-X3-C motif) ligand 1 ( MGI:1097153)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003381. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003381_01	euxassay_003381_02	euxassay_003381_03	euxassay_003381_04

euxassay_003381_05	euxassay_003381_06	euxassay_003381_07	euxassay_003381_08	euxassay_003381_09

euxassay_003381_10	euxassay_003381_11	euxassay_003381_12	euxassay_003381_13	euxassay_003381_14

euxassay_003381_15	euxassay_003381_16	euxassay_003381_17	euxassay_003381_18	euxassay_003381_19

euxassay_003381_20	euxassay_003381_21	euxassay_003381_22	euxassay_003381_23	euxassay_003381_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10894_wholemount_strong.wlz

10894_wholemount_moderate.wlz

10894_wholemount_weak.wlz

10894_wholemount_possible.wlz

10894_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10894_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

homogeneous

weak expression: see section 12 13 14 15 16 17

epidermis

moderate

regional

moderate expression: see section 01 02 05 13 20 21 22 weak expression: see section 03 04 06 07 08 09 10 11 12 14 15 16 17 18 19 23 24

vibrissa

moderate

regional

moderate expression: see section 02 05 20 21 22 weak expression: see section 03 04

telencephalon mantle layer

moderate

regional

moderate expression: see section 24 weak expression: see section 01 02 03 04 05 07 08 20 21 22 23

telencephalon marginal layer

weak

regional

weak expression: see section 01 02 03 04 05 07 08 09 20

medulla oblongata basal plate ventricular layer

weak

regional

weak expression: see section 09 12

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 14 not examined expression: see section 20

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 03 04 05 20 weak expression: see section 06 07 08 09 21 22 23

pons ventricular layer

weak

regional

weak expression: see section 12

ventral grey horn

moderate

regional

moderate expression: see section 11 12 13 16 18 weak expression: see section 14 15 17

naris

weak

regional

weak expression: see section 10 13

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 07 08 09 10 11 15 17

nasal cavity respiratory epithelium

weak

regional

weak expression: see section 10 11 14 16

esophagus

weak

regional

weak expression: see section 14 15

rectum

moderate

regional

moderate expression: see section 12

testis

moderate

regional

moderate expression: see section 20 weak expression: see section 05 06 07 21

thyroid cartilage

weak

regional

weak expression: see section 13 14 15

trachea

weak

regional

weak expression: see section 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6204
Entity Detected:	Cx3cl1, chemokine (C-X3-C motif) ligand 1 ( MGI:1097153)
Sequence:	sense strand is shown >T6204 GCCCAGCCTCCTGGCCCGCCGAATTCCTGCACTCCAGCCATGGCTCCCTCGCCGCTCGCGTGGCTGCTGC GCCTGGCCGCGTTCTTCCATTTGTGTACTCTGCTGCCGGGTCAGCACCTCGGCATGACGAAATGCGAAAT CATGTGCGACAAGATGACCTCACGAATCCCAGTGGCTTTGCTCATCCGCTATCAGCTAAACCAGGAGTCC TGCGGCAAGCGTGCCATTGTCCTGGAGACGACACAGCACAGACGCTTCTGTGCTGACCCGAAGGAGAAAT GGGTCCAAGACGCCATGAAGCATCTGGATCACCAGGCTGCTGCCCTCACTAAAAATGGTGGCAAGTTTGA GAAGCGGGTGGACAATGTGACACCTGGGATCACCTTGGCCACTAGGGGACTGTCCCCATCTGCCCTGACA AAGCCTGAATCCGCCACATTGGAAGACCTTGCTTTGGAACTGACTACTATTTCCCAGGAGGCCAGGGGGA CCATGGGGACTTCCCAAGAGCCACCGGCAGCAGTGACCGGATCATCTCTCTCAACTTCCGAGGCACAGGA TGCAGGGCTTACGGCTAAGCCTCAGAGCATTGGAAGTTTTGAGGCGGCTGACATCTCCACCACCGTTTGG CCGAGTCCTGCTGTCTACCAATCTGGATCTAGCTCCTGGGCTGAGGAAAAAGCTACTGAGTCCCCCTCCA CTACAGCCCCATCTCCTCAGGTGTCCACTACTTCACCTTCAACCCCAGAGGAAAATGTTGGGTCCGAAGG CCAACCCCCATGGGTCCAGGGACAGGACCTCAGTCCAGAGAAGTCTCTAGGGTCTGAGGAGATAAACCCA GTTCATACTGATAATTTCCAGGAGAGGGGGCCTGGCAACACAGTCCACCCCTCAGTGGCTCCCATCTCCT CTGAAGAGACCCCCAGCCCAGAGCTGGTGGCCTCGGGCAGCCAGGCTCCTAAGATAGAGGAACCCATCCA TGCCACTGCAGATCCCCAGAAACTGAGTGTGCTTATCACTCCTGTCCCCGACACCCAGGCAGCCACAAGG AGGCAGGCAGTGGGGCTACTGGCTTTCCTTGGTCTTCTTTTCTGCCTAGGGGTGGCCATGTTTGCTTACC AGAGCCTTCAGGGCTGTCCCCGCAAAATGGCGGGGGAAATGGTAGAAGGCCTCCGCTACGTCCCCCGTAG CTGTGGCAGTAACTCATACGTCCTGGTGCCAGTGTGAGCTGCTTGCCTGCCTGCCTGTGTCCAGAGTGTG ATTCGGACAGCTGTCTGGGGACCCCCCCCCATCCTCATACCCACCTTCATCCACGCTGGGGAAATGGGAA TGGAGAAGCTGGACCCTCCAGGGGCTGTGGGCTCCATCCAATCCCCCCTCCCCCGAGGGGTGGCCCCGGA GGCCACCCTAGACCACTATTCACTTATCAGAGACAGAGCAGGTGACCTTCCAGCTCCTCTATATTTGAAA GAATCCTCTGCTGCTGGCTGGTTAGAGGGGCCCTTGACACCCCAACTCCAGTGAACAATTATTTATTGGA TTCCCAGCCCCTGCGACGACACCTGTTTCCCGCGCGCACCGTGGTCCGCCCATATCACAAGCAGCAGGCC AGGCCTATCTGCCTGTCCCCCTGACCTCCTTGTGTCTCCTGGCTTTGCTGCAGTCGCCAGCCCTTCTCCT CCCCGGCCAGCTGCGGTGCTATCTGCCCTATGTCTCCCTCTATCCCCTGTACAGAGCGCACCACCATCAC CATCAACACCGCTGTTGTGTCTTTTCTTGCATGAGGTTAAAGCTGTGTTTTCTGGAGCTCTCCGGGAAGG GAGACAAGCTTGCGAGAGGGTTTAAAGTGTTCCTCCCCAGACTTGGATGTGCTGTGAAGGCATGCTGCGT CTGAAGGAAGGGTCCAGTCCCCACTCGGCTACCAGCACCACAAAGTGCCCCACCTGTAAAAGGAAAGAAA CGTGGTCCAGAGCTGGCAATAACCTATGGCCCTGACATCATCACTTTCTCTGAGATCCTTGTCTCCACCC CTGGGTGCAACCCCACCCCTTATCAACATTAATAGTCACTGCCATTCCACTGGACTGACATTTTTGTACC CTGTGATTCTGAGGGCTGGCAAGGAGTGGCTTGAGAGTGCAGATCGTACCCTGTATGCCCCCCCCCCAAA TGGAGGCTGAGTTGGGGACTTGCAGGAACAGAGGCCAACTCAGATGGCTTCCCCTGTGTTCTCACTAGAA ACCCCTCCCCCATGCACCAAGGTGACAGTCACAGGTCTGCCCTGGCTAAAGGACAAGCCACATAGGAAAG ATTAGGACAAGCCCCTCGGAGGCAGAGGATCCAGGGTAAACCCCTGGAGTGGCCACAAACCCAATTTCAG TGTAGGGACTTGTGCATGTGTGTACTTGCATAGTCAGACAGAGGCTGCCAGGGTCCTTTCCTGTCTCTGA GAGCAGTGTTCACGCCAAGGACTCACCTTTGCCCCCATTGCAGGCAGGGCCAGAACTCCCATAGCATTCT CCAAGAGCCCTGTGACATTTTCTGGAAGGGACTCTGCCCTGGGCGCAAAGTGACTGCTGAAGCAAGGAGC AGCTGAGCAGCACCCCAGCGGAGCTGAGCCGGCAGGCCACGCCCCTCGGGGGGGGGCATTTCTACCCACC CTGCTCTGAATAGCTCCAACTTCACCTTAGGAGCCTCCCAGGGGCGAGCTTCACCCAGAAGCCAGTGACT CACTCCTTGATTGGTGGAAGCTCAGTTGGCTCCTGAGAGTGAGGAAGCCAACCCTTTGTCGACCCTCCTC CTGGGAAGCCTGTGGGCGGCTCTGATCATGCTCCACAGAACCAGTTGTAGGCCTGAGCCGCAGCAGCCCG AGTGCACTATATCCTGGCTCCTTCGGTGGGGAACCTTTAAGGGTTGGGACACCCGTCATCGGACTTTGTT GGTTCCTCCCTCCCAGAGCAGAATGTGGGCCGTAACAATCTGAGGAGGACTTTGAAAGTTGTTGATCCTT TAGGGTTTTTTTTCAAGCATCATTACCAATGTCTGTGCCATTTTGTATTTTACTAATAAAATTTTAAAGT CTTGTGAATTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498747 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498747 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10891 same embryo
	EMAGE:10893 same embryo
	EMAGE:10889 same embryo
	EMAGE:10892 same embryo
	EMAGE:10890 same embryo
	EurExpress:euxassay_003381 same experiment
	MGI:4824128 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source