EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10895

Aarsd1 alanyl-tRNA synthetase domain containing 1 ( MGI:1916934)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003301. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003301_01	euxassay_003301_02	euxassay_003301_03	euxassay_003301_04

euxassay_003301_05	euxassay_003301_06	euxassay_003301_07	euxassay_003301_08	euxassay_003301_09

euxassay_003301_10	euxassay_003301_11	euxassay_003301_12	euxassay_003301_13	euxassay_003301_14

euxassay_003301_15	euxassay_003301_16	euxassay_003301_17	euxassay_003301_18	euxassay_003301_19

euxassay_003301_20	euxassay_003301_21	euxassay_003301_22	euxassay_003301_23	euxassay_003301_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10895_wholemount_strong.wlz

10895_wholemount_moderate.wlz

10895_wholemount_weak.wlz

10895_wholemount_possible.wlz

10895_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10895_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 moderate expression: see section 09 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6193
Entity Detected:	Aarsd1, alanyl-tRNA synthetase domain containing 1 ( MGI:1916934)
Sequence:	sense strand is shown >T6193 CCGGCTTTTCTGCGCGCGCGGGTCTCTCCGGCCTAGGAGACCGCGATGGCGTTCCTGTGTCAGCGCGACA GCTACGCACGGGAGTTCACCACCACCGTGGTCTCCTGTAGTCCCGCCGAGCTGCAGACGGACGCGAGCGG CGGCAAGAAAGAAGTGTTGAGCGGATTCCATGTGGTTCTGGAAGACACGCTGCTTTTCCCCGAGGGCGGG GGACAGCCTGATGACCGTGGTACCATCAATGACATCTCTGTGCTGAGGGTGACCCGTCGTGGGGCCCAGG CCGATCACTTCACGGAGTCACCTCTGTCCCCTGGGAGTCAAGTCCAGGTCCGGGTGGACTGGGAGCGGAG GTTTGACCACATGCAGCAGCATTCAGGGCAACATCTCATCACCGCGGTTGCTGACCTTCTCTTCGGGCTG AAGACAACGTCATGGGAGTTAGGGAGACTCCGGAGTGTGATTGAGTTGGACAGCCCTTCTGTGACTGCTG AGCAGGTGGCTGCTATCGAGCAGAGTGTCAATCAGAAAATCAGAGACCGGCTGCCTGTGAGTGTTCGAGA GCTGAGCCTGGATGACCCTGAGGTGGAGCAGGTGAGGGGTCGGGGTTTGCCCGATGATCATGCTGGGCCC ATTCGAGTTGTTACCATCGAAGGTGTAGACTCCAACATGTGCTGTGGGACGCACGTGAGCAATCTCAGTG ACCTTCAGGTCATTAAGATTCTGGGGACTGAGAAAGGGAAAAAGAACAAAAGCAACCTGATATTTCTGGC TGGGAATCGGGTACTGAAGTGGATGGAGCGAAGTCATGGAAGTGAAAAGGCCTTGACCTCACTACTTAAG TGTGGCGTTGAGGATCATGTGGAAGCAGTAAAAAAGCTCCAGAATGCCACCAAGCTCCTGCAGAAGAACA ACCTGAACCTCCTTAGAGACCTGGCTGTGCACACTGCCCACAGCCTCAGGAGCAGCCCAGCCTGGGGTGG TGTGGTCACACTACACAGGAAAGAGGGTGATTCTGAATTCATGAATATCATTGCTAATGAGATTGGATCG GAGGAGACCCTCCTGTTCTTAACTGTGGGGGATGAGAAGGGTGCTGGGCTCTTCTTACTGGCAGGCCCGG CAGAGGCTGTGGAAACCCTGGGGCCCAGGGTGGCTGAAGTCTTGGAAGGCAAAGGAGCAGGGAAGAAGGG CCGCTTCCAGGGCAAAGCCACCAAGATGAGCCGCCGGGCAGAGGCGCAGGCGCTTCTGCAGGACTATGTC AGCACACAGAGTGCTGAGGAGTGAGGGGCCAGGACGTCGTCCCTGTGACCAACAGTAAAATATTGTGACT CAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3497962 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3497962 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10898 same embryo
	EMAGE:10896 same embryo
	EMAGE:10897 same embryo
	EMAGE:10899 same embryo
	EMAGE:10900 same embryo
	EurExpress:euxassay_003301 same experiment
	MGI:4822856 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source