EMAGE mouse gene expression spatial database

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EMAGE:10896

Lima1 LIM domain and actin binding 1 ( MGI:1920992)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003304. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003304_01	euxassay_003304_02	euxassay_003304_03	euxassay_003304_04

euxassay_003304_05	euxassay_003304_06	euxassay_003304_07	euxassay_003304_08	euxassay_003304_09

euxassay_003304_10	euxassay_003304_11	euxassay_003304_12	euxassay_003304_13	euxassay_003304_14

euxassay_003304_15	euxassay_003304_16	euxassay_003304_17	euxassay_003304_18	euxassay_003304_19

euxassay_003304_20	euxassay_003304_21	euxassay_003304_22	euxassay_003304_23	euxassay_003304_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10896_wholemount_strong.wlz

10896_wholemount_moderate.wlz

10896_wholemount_weak.wlz

10896_wholemount_possible.wlz

10896_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10896_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6197
Entity Detected:	Lima1, LIM domain and actin binding 1 ( MGI:1920992)
Sequence:	sense strand is shown >T6197 GCGCAGGACCGTGGCCGTGCAGCGCCGAGCCCCGACGGCTTTCTAGTCAGCCCACAGGTGTCTCAGAGTC TGTAGACAAGATGGAATCGACTCCATTTAATAGACGCCAGTGGACTTCCCTGTCATTGAGAGTAACAGCC AAAGAGCTTTCTCTTGTCAACAAGAACAAGTCATCCGCAATTGTGGAAATATTCTCCAAGTACCAGAAGG CTGCTGAAGAAGCCAACATGGAAAGGAAGAAAAATAACCCTGAAAGTCTGCCCCAGCACTTTAGAAGAGG GACCCTGTCTGTGTTAAAGAAGAAGTGGGAGAACCCGGTGGCTGGGGCAGAATTCCACACAGACTCACTG CCAAACAGCAGCAGTGAGGGTGGGCACACAGCGGACTACCCTCCTGCTGAAGTGACGGACAAGCCTGCTC CTGGAGTCAGAGCTGACCGGGAAGAGCACACCCAGCCCAAACCTAGATTTGGATCTCGTCCGGAAGCAGT TATCCAGAGCCGGTATCCTCGCTCAGAGAACAGCCACGATTTTAAAGCCCAGGCCACGGAGAGCCAAAAA ATGGAAAACTGTCTGGGAGATTCCAGGCATGAAGCAGAGAAGCCAGAGACGAGCGAAAACACAGAAACTT CAGGCAAAATAGAGAAATACAACGTTCCACTGAATAGACTGAAGATGATGTTTGAGAAAGGTGAACACAA CCAAACCAAGAGTCTCTGGACCCAAAGCCGAAATGCGGGTGGAAGGAGGCTCTCTGAAAACAACTGTTCC CTGGATGACTGGGAAATAGGTGCGGGTCATTTGTCTTCCTCTGCATTCAACTCGGAGAAAAACGAGAGTA AGAGGAATCTGGAGCTGCCACGCCTCTCAGAAACCTCCATAAAGGACCGCATGGCCAAGTACCAGGCTGC AGTGTCCAAGCAGAGCAGCCCAGCCAGCTACACAAATGAGCTGAAAACCAGTGAAAGCAAAACTCATAAA TGGGAACAGAAGGAGAATGTGCCCCCAGGTCCCGAGGCCTGCAGCGTCCATCAGGAAGGAAGCAAGGTTT CTACAACTGAGAATAGTCTAGTGGCCCTCTCTGTCCCTGCTGAAGATGACACCTGTAACTCCCAGGTGAA GAGCGAGGCCCAGCAGCCTATGCACCCTAAGCCGCTGAGTCCTGACGCCAGGACCTCCAGTCTTCCGGAA AGTTCTCCTTCCAAAACAGCGAAGAAGTTTCAGGCGCCGGCAAAGGAGAGCTGCGTGGAGTGTCAGAAGA CGGTGTACCCCATGGAACGGCTCCTGGCCAACCAGCAGGTGTTTCACATCAGCTGTTTCCGATGCTCCTA CTGCAACAACAAGCTCAGTCTAGGAACATATGCATCCTTACATGGACGAATCTACTGTAAGCCTCACTTC AATCAACTCTTCAAGTCCAAAGGCAACTATGACGAGGGCTTTGGGCACAAGCAGCATAAGGATCTGTGGG CAAGCAAGAGCGACAATGAGGAGACTTTGGGGAGACCAGCCCAGCCGCCTAATGCAGGGGAGAGCCCCCA TAGCCCGGGTGTAGAAGATGCCCCCATCGCCAAGGTCGGCGTGCTGGCGGCAAGTATGGAAGCCAAGGCC TCTTCTCAGAGGGAGAGGGAGGATAAGCCTGCGGAGACCAAGAAGCTGAGGATCGCCTGGCCTCCGCCAG CCGAGCTGGGCGGTTCCGGAAGTGCCCTGGAGGAAGGGATCAAAGTATCGAAGCCCAAGTGGCCTCCGGA GGATGACGTCTGCAAGACGGAGGCCCCGGAGGATGTAGATCTGGACCTGAAGAAGCTGCGGCGGTCTTCG TCCCTGAAGGAGAGGAGCCGCCCGTTTACGGTAGCAGCTTCCTTTCGAACCTCTTCCATCAAGAGCCCCA AGGCCTCTTCGCCATCTCTCAGGAAAGGTTGGAGCGAGTCCGAGCAGAGTGAGGAGTTTGGAGGAGGGAT AGCGACGATGGAAAGGAAACAAACGGAAAATGCCAGACCCTCTGGGGAGAAAGAGAATGTGGGAAAATCA CGCTGGCAGGGCGAGGAAGTGCCGAGGAGCAAGGACCGTAGTTCTTTTGAGCTGGAGAGTGAGAATTTTA TGGAAAATGGAGCAAACATAGCTGAAGATGACAACCATGTCCACGCACAGCAGTCCCCGTTAGAACCCGA GGCTCCGGGTTGGTCTGGCTTTGTAGACACCACGGCTGCTAAAGAATTCACTACCCAGAATCAAAAATCC CAAGATGTCGGGTTCTGGGAGGGAGAAGTGGTCCGAGAGCTGTCTGTGGAGGAACAGATAAAGAGAAACC GGTACTATGATGAGGACGAGGATGAAGAATGACCGGCCGCCATGGTTCTGGCCTTAGATTTTGGTTAGCA ACTCACTGCCCTTTATCAAGTGGTGCGCAGAAACAGGCAGCTTGGCGCCAAGGGTCATTCATGTGGGAGC AACTTTGGAAATGAATTCCCGCTAGCAAAACAGGGCAAAAACAAGCAAAAACAAAACAGACAAACAAACT CACCCAAAACCAATTCTAAGCGCATTGTCAAAACACCAGTGATCTTTTAAGCCTGGTGTGGTGGTAAACA CCTTTAATCCCAGCACTCCACTCGGGAGGCAGAGGCAGAGGCAGGTGGGTCTCTCTGAGTTCCAGGACAG CCAGGGCTACAAAGAGAAACCTTGCCTCAAAACAAACAAACAAACAAATCCCTCCATTTTTTCAGGGAAG GGCTAAGTAGGGATTTCTCTCACTGATGCGCCTGCCATCCACTGCATTCCCCAAATCAAGATTGGTGTGG TTAGATATTTAGAGGCTTGTTTTCACACTACAGAGACCATTGTGGAATTAGAGACACACACAACACATTT AGGAGCCCACCTCATGACCAGAGGCACTTTAGTATAAAGGGCACAGTGTAGCTATTTTACCAATTTCATT TTTTAGTACTCAATTGCTGAAAATCAAATATTTAGTCTTTCAAGCTTTTGAAATGCATTCTCTTCCTCTG GTAGATAGGGAAAGCTTACTGTCTTTCATCCTACATCCTGTATTTTTAATCACTGAGGCACAAAACAGCA ACCTTTGGCCAAGTGAAAGGCGCTGGTTACTGAAGCCGGAGCAGCAGTGTTCAATCCCCAAAACCTACGT AAAGGTGAGAGAGCAGACTGCACAGAGCTGTCCACACGTGCCCATCATCAACAATAAGAAACAAGTTAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498138 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498138 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10895 same embryo
	EMAGE:10898 same embryo
	EMAGE:10897 same embryo
	EMAGE:10899 same embryo
	EMAGE:10900 same embryo
	EurExpress:euxassay_003304 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source