EMAGE mouse gene expression spatial database

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EMAGE:10897

Rbp1 retinol binding protein 1, cellular ( MGI:97876)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003315. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003315_01	euxassay_003315_02	euxassay_003315_03	euxassay_003315_04

euxassay_003315_05	euxassay_003315_06	euxassay_003315_07	euxassay_003315_08	euxassay_003315_09

euxassay_003315_10	euxassay_003315_11	euxassay_003315_12	euxassay_003315_13	euxassay_003315_14

euxassay_003315_15	euxassay_003315_16	euxassay_003315_17	euxassay_003315_18	euxassay_003315_19

euxassay_003315_20	euxassay_003315_21	euxassay_003315_22	euxassay_003315_23	euxassay_003315_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10897_wholemount_strong.wlz

10897_wholemount_moderate.wlz

10897_wholemount_weak.wlz

10897_wholemount_possible.wlz

10897_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10897_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb

moderate

regional

moderate expression: see section 01 02 03 04 23 24

hindlimb

moderate

regional

moderate expression: see section 01 02 03 04 21 22 23 24

brain

moderate

regional

moderate expression: see section 06

hypothalamus mantle layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15 weak expression: see section 16

thalamus mantle layer

weak

regional

weak expression: see section 08 09 10 11 15 16

telencephalon

weak

regional

weak expression: see section 24

corpus striatum

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23 weak expression: see section 24

olfactory lobe

moderate

regional

moderate expression: see section 11 weak expression: see section 12 17 18

hindbrain

moderate

regional

moderate expression: see section 06 07 11 12 13 15 16 17 18 weak expression: see section 08 09 10 14

viscerocranium

moderate

regional

Expression in the turbinate bone.

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6195
Entity Detected:	Rbp1, retinol binding protein 1, cellular ( MGI:97876)
Sequence:	sense strand is shown >T6195 GCCGCCCCGCGGAGCCCCGTGTCCCCCGAGGGCGCCCATTTCCGGGCCGTCCATCACCCGGAGTGCATTG GCAGCAACCGTCCGGGCCGAACCCACAGCCGCTCCCGCCTTCTTGGTGTCAGGCGATCCCCAGTCTGTCC CAAAAATGCCTGTGGACTTCAACGGGTACTGGAAGATGCTGAGCAATGAGAATTTCGAGGAGTACCTGCG CGCGCTCGACGTCAACGTGGCCTTACGCAAAATCGCCAACTTGCTGAAGCCAGACAAAGAGATCGTGCAG GATGGCGACCACATGATCATCCGCACGCTGAGCACTTTTCGGAACTATATCATGGACTTCCAAGTTGGGA AGGAGTTTGAGGAAGATCTGACAGGCATAGACGACCGCAAGTGCATGACCACTGTGAGCTGGGATGGTGA CAAACTCCAGTGTGTGCAGAAGGGAGAGAAGGAGGGACGTGGCTGGACGCAGTGGATCGAGGGTGATGAA CTTCACCTGGAAATGAGAGCTGAGGGTGTGATCTGCAAGCAAGTGTTTAAGAAAGTACACTGAGCAGCCC GAACAGCCCGAGGAGATAACCTTGGTCTTCAGGAACAAGTGGGATGGGCCTGTGGTCAGGAGCCCCCTCT GCCTAACATGGGGACCGAAACGCATACCCACCCCAGGCTTCTGCCAGCGAAGTCTCTCTCTTTGCCTGGT CTCTTTTCCTTTACTTAAAATGAAGTGACCCCAATAAAAGTGATCTCTGTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498107 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498107 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10895 same embryo
	EMAGE:10898 same embryo
	EMAGE:10896 same embryo
	EMAGE:10899 same embryo
	EMAGE:10900 same embryo
	EurExpress:euxassay_003315 same experiment
	MGI:4827657 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source