EMAGE mouse gene expression spatial database

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EMAGE:10908

Iigp1b interferon inducible GTPase 1B ( MGI:4366520)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006540. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006540_01	euxassay_006540_02	euxassay_006540_03	euxassay_006540_04

euxassay_006540_05	euxassay_006540_06	euxassay_006540_07	euxassay_006540_08	euxassay_006540_09

euxassay_006540_10	euxassay_006540_11	euxassay_006540_12	euxassay_006540_13	euxassay_006540_14

euxassay_006540_15	euxassay_006540_16	euxassay_006540_17	euxassay_006540_18	euxassay_006540_19

euxassay_006540_20	euxassay_006540_21	euxassay_006540_22	euxassay_006540_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10908_wholemount_strong.wlz

10908_wholemount_moderate.wlz

10908_wholemount_weak.wlz

10908_wholemount_possible.wlz

10908_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10908_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

spotted

strong expression: see section 11 moderate expression: see section 10 12 13 14 15

thyroid gland

moderate

regional

moderate expression: see section 09 10 11 14 15

esophagus

strong

regional

strong expression: see section 12

lung

strong

regional

strong expression: see section 09 10 20 21 22 moderate expression: see section 03 04 05 06 07 08 11 12 13 14 15 16 17 18 19 23

trachea

strong

regional

strong expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6775
Entity Detected:	Iigp1b, interferon inducible GTPase 1B ( MGI:4366520)
Sequence:	sense strand is shown >T6775 GCAGAAGTAGACTCAAGCCAATATCGCACCAGAGGGCTATTCCTTTCCCGAGCAGTGAGTATATCCACTG GACACAGGATTTTCTGTGCCTTTATTTGCTTGAAGTCATGGGTCAGCTGTTCTCTTCACCGCAGAGTGAG CACCAAGATTTGGCCTCCAGCTTTACTGAATATTTTAAGAAATTTAAGATGGGAAACAAAATCATTTCTC AGGATATCATCAGTTTGGTTGAATTAAGTATGGCAAAAGGGAACATTCAGGGGGCAAACTCTGCAATCAA GAATGCATTAAAAGAAATCGATAGTACCCCGCTCAATGTTGCTGTCACTGGGGAGAGTGGGTCAGGGAAG TCCAGCTTCATCAACACCCTGAGAGGCATTGGGCATGAAGAGAAAGGTGCAGCTAAAACTGGGGTGATGG AGGAAACCATGGAGAGACATCCATACAAACACCCCAATATGCCCAATGTGGTTTTTTGGGACCTGCCTGG GATTGGAACCACAAAGTTCCCACCAAAAACTTACCTGGAGAAAATGAAGTTCTATGAGTATGACTTCTTC ATTATTATTTCGGCCACACGCTTCAAGAAAAATGACATAGACCTTGCCAAAGCAATCAGCATGATGAAAA AGGAATTCTATTTTGTGAGAACCAAGGTGGACTCTGACTTAAATAATGAAGAGGATTTCAAACCTCAGAA TTTTGACAGAGAAAAAGTCCTGCAGAACATCCGGCTTAACTGTGTGAACAACTTTAAGGAGAATGGCATT GCTGAGCCACCGATCTTCCTGGTCTCTAACAAGAATGTTTGTCACTATGACTTTCCTGTCCTGATGGACA AGCTGATAAGTGACCTCCCTGTTTACAAGAGACACAATTTTATGCTCTCCTTACCCAATATTACAGAATC AGCCATTGAAAAGAAACGGCAATTTCTGAAGCAGAGGATTTGGCTGGAAGGGTTTGCTGCTGACCTAATG AGTATCATCCCTACACTGACCTTTCTCTTGGACAGTGATTTGGAGACTCTGAAGAAAAGCATGAAGTTCT ACCGCACTGTGTTTGGAGTGGATGAAGCATCTTTGAAAAGTTTGGCTACAGCCTGGAAAATCCCGGTGGA TCAGGTGGAGGCCATGATGAAATCTCCTGCTGTGTTCAAACCTACAGATGAAGAAACAATACAAGAAAGG CTTTCCAGATATGTTCGGGAGTTCTGTTTGGCTAATGGGTTCTTAGTTACTAAAAATCATTATCTTAGAG AAATATTTTACCTGAAATATTATTTCCTTGACATGGTGACTGAGGACGCTAAAACTCTTCTCAAAGAGAT ATGTTTAAGAAACAAGTTGCTTTCTAATTAGGCTGAAGTCCTGTGACTGTCAAGAAATTAGAATCAATGA CAGAAAAACAGGACACTGATACTGTAAAACAATATACTGTGTTTATCCAGGTTATTCATGCTTTATCCAT ATTTAGTGATTATGTATCCACATTACCTAGAGCAAGGTGATCAGATATGTGTAAATAAAGTGATAGCTAA AAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3675329 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3675329 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10910 same embryo
	EMAGE:10907 same embryo
	EMAGE:10909 same embryo
	EurExpress:euxassay_006540 same experiment
	MGI:4825552 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source