EMAGE mouse gene expression spatial database

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EMAGE:10939

Ubb ubiquitin B ( MGI:98888)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006591_01	euxassay_006591_02	euxassay_006591_03	euxassay_006591_04	euxassay_006591_05

euxassay_006591_06	euxassay_006591_07	euxassay_006591_08	euxassay_006591_09	euxassay_006591_10

euxassay_006591_11	euxassay_006591_12	euxassay_006591_13	euxassay_006591_14	euxassay_006591_15

euxassay_006591_16	euxassay_006591_17	euxassay_006591_18	euxassay_006591_19	euxassay_006591_20

euxassay_006591_21	euxassay_006591_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10939_wholemount_strong.wlz

10939_wholemount_moderate.wlz

10939_wholemount_weak.wlz

10939_wholemount_possible.wlz

10939_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10939_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8166
Entity Detected:	Ubb, ubiquitin B ( MGI:98888)
Sequence:	sense strand is shown >T8166 AAGCAGTGGTAACAACGCAGAGTACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG AGGGTGTTTCGACGCGCTGGGCGGTTTGTGCTTTCATCACATTTGTTAACAGGTCAAAATGCAGATCTTC GTGAAGACCCTGACCGGCAAGACCATCACCCTAGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGG CCAAGATCCAGGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCAAGCAGCTGGA AGATGGCCGCACCCTCTCTGATTACAACATCCAGAAGGAGTCAACCCTGCACCTGGTCCTTCGCCTGAGA GGTGGCATGCAGATCTTCGTGAAGACCCTGACCGGCAAGACCATCACCCTGGAGGTGGAGCCCAGTGACA CCATCGAGAACGTGAAGGCCAAGATCCAGGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTT TGCCGGCAAGCAGCTGGAAGATGGCCGCACCCTCCCTGATTACAACATCCAGAAAGAGTCAACCCTGCAC CTGGTCCTCCGTCTGAGGGGTGGCATGCAGATCTTCGTGAAGACCCTGACTGGCAAGACCATCACCCTGG AGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATCCAGGATAAAGAGGGCATCCCCCCTGA CCAGCAGAGGCTGATCTTTGCCGGCAAGCAGCTGGAAGATGGCCGCACCCTCTCTGATTACAACATCCAG AAGGAGTCAACCCTGCACCTGGTCCTTCGCCTGAGAGGTGGCATGCAGATCTTCGTGAAGACCCTGACCG GCAAGACCATCACCTTGGAGGTGGAGCCCAGTGTCACCATCGAGAATGTGAAGGCCAAGATCCAGGATAA AGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCAAGCAGCTAGAAGATGGCCGCACTCTC TCTGATTACAACATCCAGAAAGAGTCGACCCTGCACCTGGTCCTCCGTCTGAGGGGTGGCTATTAATTAT TCGGTCTGCATTCCCAGTGGGCAGTGATGGCATTACTCTGCACTCTAGCCACTTGCCCCAATTTAAGTTT AGAAATTACAAGTTTCAATAATAGCTGAACCTCTGTTAAAAATGTTAATAAAGGTTTTGTTGCATGAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6435236 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6435236 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10942 same embryo
	EMAGE:10941 same embryo
	EMAGE:10940 same embryo
	EurExpress:euxassay_006591 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source