EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10942

Usp16 ubiquitin specific peptidase 16 ( MGI:1921362)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003554. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003554_01	euxassay_003554_02	euxassay_003554_03	euxassay_003554_04

euxassay_003554_05	euxassay_003554_06	euxassay_003554_07	euxassay_003554_08	euxassay_003554_09

euxassay_003554_10	euxassay_003554_11	euxassay_003554_12	euxassay_003554_13	euxassay_003554_14

euxassay_003554_15	euxassay_003554_16	euxassay_003554_17	euxassay_003554_18	euxassay_003554_19

euxassay_003554_20	euxassay_003554_21	euxassay_003554_22	euxassay_003554_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10942_wholemount_strong.wlz

10942_wholemount_moderate.wlz

10942_wholemount_weak.wlz

10942_wholemount_possible.wlz

10942_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10942_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6270
Entity Detected:	Usp16, ubiquitin specific peptidase 16 ( MGI:1921362)
Sequence:	sense strand is shown >T6270 GGCTCCGGGCCCGTCGCGGGAGGCAGTTATGGGCGCCGTCGCCCCGGATTGTAACTTTGTGTCAGCGTGC AGTCCATAAAGTGCCAGCATGGGGAAGAAACGGACCAAGGGGAGAAGTGCTCCAGACACGGTGGCCTCAG AGTCTGCAGAACCAGTGTGCAGACACCTTAGAAAAGGGTTGGAACAAGGTAATTTGAAAAAAGCTTTAGT AAATGTGGAGTGGAATATCTGCCAAGACTGTAAGACTGACAATAAAGTGAAAGATAAACCTGAGGAGGAA GCAGAAGACCCTTCGGTTTGGCTCTGTCTTAAATGTGGCCATCAGGGCTGTGGCAGAGATTCTCAGGAGC AGCATGCCTTGAAGCACTACACGACACCGAGATCCGAGCCTCACTACCTGGTGCTCAGTCTGGACAACTG GAGCGTCTGGTGCTACAAGTGTGACGAGGAAGTCAAGTACTGTAGCTCAAACCGATTGGGCCAAGTGGTT GATTATGTTAGAAAACAAGCTGGCGTAAGAACTTCAAAACCAGCAGAGAAAAATAATGGACACATTGAGC TCGAAAATAAAAAATTGGAGAAAGAGAGTAAAAATGAACAAGAGAAAGAGAAATCGGAAAACCTGGCTAA AGAAACTATTCCCATGGACTCTGCTTCCCAGATAACTGTGAAAGGACTCAGTAATTTGGGGAATACTTGT TTCTTCAATGCAGTTATGCAGAACTTGTCACAAACGCCAGTGCTTAGAGAACTACTAAAAGAAGTGAAGA TGTCTGGAACGATTGTAAAAATAGAGCCACCTGATCTGGCACTAACAGAACCTTTAGAAGTAAACCTCGA GCCTCCAGGTCCTCTTACTTTAGCCATGAGCCAGTTTCTCAGTGAGATGCAAGAGAACAAAAAGCGAGTT GTGACACCTAAAGAGCTCTTTTCTCAGGTCTGTAAAAAAGCAACACGTTTTAAAGGGTACCAGCAACAAG ACAGCCAGGAGCTGCTTCGCTACCTACTGGATGGGATGAGAGCGGAAGAACACCAAAGAGTGAGTAAAGG AATTCTTAAAGCATTTGGTAATTCTACTGAAAAATTGGATGAAGAAGTAAAAAATAAAGTTAAAGATTAT GAAAAGAAAAAGGCAATCCCGAGTTTTGTGGACCGCATCTTTGGTGGCGAGCTGACTAGTACGATCATGT GTGATGAATGCAGGACTGTCTCCTTAGTGCATGAATCGTTCCTTGATTTGTCTCTTCCAGTTTTAGATGA TCAGAGTGGTAAGAAAAGTATAAATGATAAAAATGTGAAAATGACAATGGAGGAAGAAGATAAAGACAGT GAGGAAGAGAAAGATGACAGCTACATGAAATCAAGGAGCGATCTTCCGTCAGGGACAAGCAAGCACCTAC AGAAAAAGGCAAAGAAGCAGGCCAAAAAGCAGGCCAAGAACCAACGAAGGCAACAAAAAATTCAAGAAAG ATTTCTTCACTTCAATGAGCTCTGCGCCACTGACTACACGGAAGACAATGAACGTGAAGCTGACACAGCA CTTGCGGGAGAAGTGGAAGTGGATACCGACTCCACCCATGGTTCTCAAGAGGAGGCCACACAGATAGAGC TGTCTGTTAACCAGAAGGATTTGGATGGCCAAGAGAGCATGATAGAAAGGACACCTGATGTGCAGGAAAG CCCAGAGGACCTAGGAGTGAAAAGTGCTAACACCGAGAGTGATCTGGGGATTGTGACGCCTGCTCCTGAA TGTCCTAGGGATTTCAATGGTGCCTTCCTGGAAGAAAGGACCAGTGGAGAACTAGACATTATCAATGGTT TAAAAAACCTTAATTTGAATGCTGCTGTTGATCCTGATGAAATAAATATAGAGATTCCGAATGACAGTCA TTCTGCACCCAAGGTATATGAGGTCATGAACGAGGACCCAGAAACTGCTTTCTGTACCCTCGCGAACCGA GAAGCGTTTAGTACTGATGAGTGTTCCATTCAACATTGCTTATATCAGTTCACCCGGAATGAGAAACTTC AAGATGCCAATAAACTGCTTTGTGAAGTGTGTTCAAGACGGCAGTGTAATGGACCAAAGGCAAATATAAA AGGTGACAGGAGACATGTTTACACCAATGCCAAGAAGCAGATGCTGGTCTCCCTCGCGCCTCCTGTCCTC ACTCTGCATTTAAAGCGATTCCAGCAGGCTGGTTTTAACCTGCGCAAAGTTAACAAACACATAAAGTTTC CAGAAATCTTAGATTTGGCTCCTTTTTGTACCCTTAAATGTAAGAATGTTGCTGAAGAAAGTACACGAGT GCTGTATTCCTTATATGGAGTTGTTGAACACAGTGGTACTATGAGGTCAGGGCATTACACTGCCTATGCG AAGGAGAGAACTGCAAGCTGTCACCTCTCCAATCTTGTTCTTCACGGTGACATTCCACAAGATTGTGAAA TGGAATCAACCAAAGGGCAGTGGTTTCACATCAGCGATACACATGTGCAAGCTGTGCCTATAACTAAAGT ACTGAACTCACAAGCATATCTCCTATTTTATGAGAGAATACTGTGATAACAAAAAGTGCTTTCTCTGGAA ATACACCTATGGCTTTTATACTGGCTATTATAACAATAAAAAGTTAAACTATAAATTATGTTCACCTAAG TAAATGACAGAAAAAAAATCATGTTTATTTATTTAAATACAGGCAAAATAATTTACAGCGGTTTTGTATT AGCATACTGGTTTTTATTCCTTCTAGTTTCAACTTTAGGAAGGATTTGAATAACTAAGTTCTGTGCTTAC TCTGACTGGGTGGTAGTGCTTGACACATCAATAAACTGATATTCCCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3582180 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3582180 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10941 same embryo
	EMAGE:10940 same embryo
	EMAGE:10939 same embryo
	EurExpress:euxassay_003554 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source