EMAGE mouse gene expression spatial database

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EMAGE:10944

Ldb1 LIM domain binding 1 ( MGI:894762)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006506. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006506_01	euxassay_006506_02	euxassay_006506_03	euxassay_006506_04

euxassay_006506_05	euxassay_006506_06	euxassay_006506_07	euxassay_006506_08	euxassay_006506_09

euxassay_006506_10	euxassay_006506_11	euxassay_006506_12	euxassay_006506_13	euxassay_006506_14

euxassay_006506_15	euxassay_006506_16	euxassay_006506_17	euxassay_006506_18	euxassay_006506_19

euxassay_006506_20	euxassay_006506_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10944_wholemount_strong.wlz

10944_wholemount_moderate.wlz

10944_wholemount_weak.wlz

10944_wholemount_possible.wlz

10944_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10944_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6787
Entity Detected:	Ldb1, LIM domain binding 1 ( MGI:894762)
Sequence:	sense strand is shown >T6787 CCGGGCTGTGGACCCCGCGGGGCGGGCGGAGGCTGTGCATGCGAGTCTTTGTGCGGCCTGCGGAGTGGAG CTCCCAGCCAGACCGGCCTGTCCCACTCGGGACATGAGCTGCTGAAGTTGGCTCCACCTTAGTCCTCACC CTTGCCAGGGTCCCTGAGGACTCCCAGGCGCTCCCCGCACCTCCCCTGACCCCCTCAGTTCTCCCAGGTT GTTCCTCAAAGTCATTCAAGCTGTACTCGCCGAAGGAGCCCCCGAACGGCAACGCCTTCCCTCCCTTCCA CCCCGGCACCATGCTGGATCGGGATGTGGGCCCAACTCCCATGTACCCACCTACATACCTGGAGCCTGGG ATCGGGAGGCACACACCATATGGTAACCAAACCGACTATAGAATATTTGAGCTTAACAAACGGCTACAGA ACTGGACAGAGGAGTGTGACAATCTCTGGTGGGATGCTTTCACAACTGAGTTCTTTGAAGATGACGCCAT GCTGACCATCACTTTCTGCTTGGAGGATGGACCAAAGAGATATACCATTGGCCGGACCCTGATACCACGC TACTTCCGAAGCATTTTTGAGGGGGGTGCCACAGAGCTGTACTACGTGCTCAAGCACCCCAAGGAGGCAT TCCACAGCAACTTCGTGTCCCTCGACTGTGACCAGGGCAGCATGGTGACCCAGCACGGCAAACCCATGTT TACCCAGGTGTGTGTGGAAGGCCGGTTGTACCTGGAGTTCATGTTTGACGACATGATGCGGATAAAGACG TGGCACTTCAGCATCCGGCAACACAGAGAGCTCATCCCCAGAAGTATCCTGGCCATGCACGCCCAGGACC CCCAGATGCTGGATCAGCTGTCCAAAAACATTACCCGGTGTGGGCTGTCCAATTCCACTCTCAACTACCT CCGACTCTGTGTGATACTAGAGCCCATGCAGGAACTTATGTCCCGCCACAAGACCTACAGCCTCAGCCCC CGAGACTGCCTCAAGACCTGCCTCTTCCAGAAGTGGCAGCGAATGGTAGCGCCTCCCGCGGAGCCCGCAC GACAGCAGCCCAGCAAACGGAGGAAACGGAAGATGTCAGGGGGTAGCACCATGAGCTCGGGGGGTGGCAA CACCAACAACAGCAACAGCAAGAAGAAGAGCCCAGCCAGCACCTTCGCTCTCTCCAGCCAGGTACCTGAT GTGATGGTGGTGGGGGAGCCCACCCTGATGGGCGGGGAGTTCGGGGACGAGGACGAGAGGCTCATCACCC GGCTGGAGAACACCCAGTTTGACGCGGCCAACGGCATTGACGACGAGGACAGCTTTAACAACTCCCCCGC ACTGGGCGCCAACAGCCCCTGGAACAGCAAGCCTCCATCCAGCCAAGAGAGCAAATCGGAGAATCCCACG TCACAGGCTTCCCAGTGAAGGCCCTACCACGGCCACCCAGCGCTCTGCCTGCCTACCCCTCACAGCTCCA GGGAGCAGAAGACGGAATAAAGAGACTGGGCGTCTGAAATGGGCAGCCTCCATCCACCCACTTCAGGGAG GGACTGGACTCGCTGGGGGCAGGTGCCAAGATTTGCCCCCCAGTTGCCCTGGGCTGGGCCTGGCCCCTGC AGAGCCTTTTGGGGGAAGGGGGTACTCATGTGGATGCCTGTGTGGACCCTGGGCCTCTGAGAAATGTCCC GACCCTCCTCTCCCCAGGGCATTTGCCCTCGTTCTTACCCCAGGTTCTGGGTGCCCCATCTTCCTGATTT CTCCCTTGGATCCTGGGGTTTATACCTACAGCCCTTAGGGGCTAAATGTTGGGGCTGTGGTTGAACGCCC TTCCCCTAGGTGGCTGTCAACAGAGGGTAAAATTCTGGGCTCCTCCCTTTTGCCACTTACCCAGCTCCAA GTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3708169 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3708169 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10948 same embryo
	EMAGE:10946 same embryo
	EMAGE:10947 same embryo
	EMAGE:10945 same embryo
	EurExpress:euxassay_006506 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source