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EMAGE:10999

Cend1 cell cycle exit and neuronal differentiation 1 ( MGI:1929898)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10999 EMAGE:10999 EMAGE:10999 EMAGE:10999 EMAGE:10999
"Pseudo-wholemount" of euxassay_001260. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001260_01 euxassay_001260_02 euxassay_001260_03 euxassay_001260_04
EMAGE:10999 EMAGE:10999 EMAGE:10999 EMAGE:10999 EMAGE:10999
euxassay_001260_05 euxassay_001260_06 euxassay_001260_07 euxassay_001260_08 euxassay_001260_09
EMAGE:10999 EMAGE:10999 EMAGE:10999 EMAGE:10999 EMAGE:10999
euxassay_001260_10 euxassay_001260_11 euxassay_001260_12 euxassay_001260_13 euxassay_001260_14
EMAGE:10999 EMAGE:10999 EMAGE:10999 EMAGE:10999 EMAGE:10999
euxassay_001260_15 euxassay_001260_16 euxassay_001260_17 euxassay_001260_18 euxassay_001260_19
EMAGE:10999 EMAGE:10999 EMAGE:10999 EMAGE:10999 EMAGE:10999
euxassay_001260_20 euxassay_001260_21 euxassay_001260_22 euxassay_001260_23 euxassay_001260_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10999Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10999_wholemount_strong.wlz
10999_wholemount_moderate.wlz
10999_wholemount_weak.wlz
10999_wholemount_possible.wlz
10999_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10999_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon marginal layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
olfactory cortex marginal layer
moderate moderate
regionalmoderate expression: see section 12 13 14 15 17 18 19 20
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 10 11 12
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 11 15 16
pons mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 14 17
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 07 10 11 12 13 18
facial vii ganglion
strong strong
regionalstrong expression: see section 04 05 06 07 20 21 22
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 06 07 18 19
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 22 23
vagus x ganglion
strong strong
regionalstrong expression: see section 08 18
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 18 19
spinal cord mantle layer
moderate moderate
homogeneousmoderate expression: see section 07 08 09 10 11 12 13 14 15 16
ventral grey horn
strong strong
regionalstrong expression: see section 09 10 11 13 14 15 16
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 09 10 16
cervical ganglion
moderate moderate
regionalmoderate expression: see section 09 17
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 11 13 14 15
dorsal root ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 15 16 17
cornea stroma
moderate moderate
regionalmoderate expression: see section 02 03 04 05 24
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 13 14 15 18 19 20 weak expression: see section 10 11 12 16 17 21
urethra of male
strong strong
regionalstrong expression: see section 17 moderate expression: see section 13 weak expression: see section 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5408
Entity Detected:Cend1, cell cycle exit and neuronal differentiation 1 ( MGI:1929898)
Sequence:sense strand is shown

>T5408
GAAGCGAATGCGCAGCGAGAAGCTGGAGGGCCCAAAGAAGACACCCATATAATGCCCAGAGGTGAGCTGT
CCTGAGCCACTGTGGTCTAGCCACCTGCCAGTCACACAAGCCCCAAACCAGCTGCCAGCAAAAAACCATC
ATGGAATCCCGAGGAAAGTCAGCCAGCAGCCCCAAGCCAGATACCAAGGTGCCTCAGGCCACAGCTGAGG
CCAAGGCCACTCCAGCTGCGGATGGGAAAGCTCCCTTGACCAAGCCTGTGAAGAAGGACACCCAAGCAGA
GAAGCAGGAGCAAGCAGCAGCCCCTGGGCCAGCCGCTACGAAGAAGACACCAGCCAAGGCAGATCCTGTA
CTTCTCAACAACCACAGCAACCTGAAGCCAGCTCCCACAGTGCCGGCAGCCCCCAGCAGTCCTGATGCAA
CCTCAGAGCCCAAGGGTCCTGGGGATGGGGCAGAGGAGGACGAGTCCAACACTGGAGGCCGAGGTCCCTG
GCCTTGTGAGAACTTGACCCCTTTGCTGGTAGCTGGGGGTGTGGCCGTGGCCACCATAGCTCTAATTCTT
GGTGTGGCCTTTCTAGCCCGGAAAAAATGATACCTGGTGTCCAGGTCGGGGCACAGAGCCACTTTCTGTA
CAGACCTGAATATATAGTGCATGCAAGGTCCTAGATATTTATCTATACATATACACCTAACAACACACAC
ACAACACACACACACACTCACACGTATATGTATGCTACATACATCCGTGCAGCAGGAAAGAAATAGACAG
TGAGCCCACCTCCACTCACCCCAGCACACCCTCTCCCAAGTTCTGGGGCAGGAGCCCAGCTCTGCAGTTT
GCAGGAAGCACTAGCGTGGGGCAAAGGGGAGAGATGCTGGGTGTGGTCTCTGAAAGATAAGTGATGGAGT
GCGCTAGCCCTGTGGGGACATTAAAGGGCATGGCTGTCTTACTCCTCTCACTTGTCTTTTGAGCAGCCAG
GAGCAGTCTGTCTGGGCCTGATGCCGGGGGACTGACCTAAGGGTGGGTGGGATTCAAATCTGCAGAGCTA
CCTCTACCCATTGTCCCTGTCCTGGTTGGATGGAAAAGGCAAAGTTAACAGCTGAGTTTGGGATGGGGTT
CCCACCTCTTCCCTCTGTCAGTGTATGCCCCAGGAGAGCACCGGAGGCTAGGACCACCTTACTTCCTGCC
CAGAGCAGCCAATTGTTCCGGGGAGGGGGTGGTTCTGAAAGCCTGGCAGGGAGGCAGGAAGCACGAAGGA
GCCACACTACAGTTCTGCCTGCCCAATATTCCCCAATACTGTCCTTCCTGCCTGCACCCAACACCCCCCC
CCTTGAGTTTAACAGCGTATAAATGCAATAACACAGGTAGAGTAGAACTGCCCGGTGAGTGGCAATCGCT
CACTGCCTGCAGGAAGACCTGCTGTGCAGCCCCTCTTTAGTGCATCTGGCCCCAGCTTGCCCCTTGGTGT
GGTGACAGTGAGCAGCTTTCCGGAAAACTGCAGTGGGCTCTGAGGAAACATTGCCCTCACCTGGCCAGTG
GCGGCGGGGACCTTGGGCCGGAGTGGAGACTGAGTTTGAAATAAACCGTTACAATATAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5366891 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5366891 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11000 same embryo
 EMAGE:10997 same embryo
 EMAGE:10996 same embryo
 EMAGE:10998 same embryo
 EMAGE:11001 same embryo
 EurExpress:euxassay_001260 same experiment
 MGI:4823806 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS