EMAGE mouse gene expression spatial database

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EMAGE:11000

Arxes2 adipocyte-related X-chromosome expressed sequence 2 ( MGI:1924226)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001229. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001229_01	euxassay_001229_02	euxassay_001229_03	euxassay_001229_04

euxassay_001229_05	euxassay_001229_06	euxassay_001229_07	euxassay_001229_08	euxassay_001229_09

euxassay_001229_10	euxassay_001229_11	euxassay_001229_12	euxassay_001229_13	euxassay_001229_14

euxassay_001229_15	euxassay_001229_16	euxassay_001229_17	euxassay_001229_18	euxassay_001229_19

euxassay_001229_20	euxassay_001229_21	euxassay_001229_22	euxassay_001229_23	euxassay_001229_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11000_wholemount_strong.wlz

11000_wholemount_moderate.wlz

11000_wholemount_weak.wlz

11000_wholemount_possible.wlz

11000_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11000_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

strong

regional

strong expression: see section 03 04 05 06 07 19 20 21 22 23

hypothalamus mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon mantle layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 13 14 15 16 18 19 20 21

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 14 15 16 17 18 19 20 21 22

pons mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 08 09 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 09 18 19 20

spinal cord mantle layer

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 17

cervical ganglion

moderate

regional

moderate expression: see section 09 17

thoracic ganglion

moderate

regional

moderate expression: see section 11 12 13 14 15

cornea stroma

strong

regional

strong expression: see section 02 03 04 05

tail dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 13 16 17 weak expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5405
Entity Detected:	Arxes2, adipocyte-related X-chromosome expressed sequence 2 ( MGI:1924226)
Sequence:	sense strand is shown >T5405 CCACGCGTCCGGCCAGAGGAAGGCAGAGGGACCGCGGAGCGAACTGCACGCCCCGTGCAGAGCCAGCCAG CCAGCCAGCCAGCCAGCGAGCGCCGGTGGAGCCCGCGTGGAGGGAGCCGCCGATTGAACACCCTGCAAGA TGAACTCCCTGCTGTCCCGGGCCAACTCGCTGTTCGCCTTCACGCTGAGCGTCATGGCGGCGCTCACCTT GGGCTGCATCCTCACCACCGCCTTCAAAGACAGGAGCGCGCCCGTGCGCCTGCACGTCTCCAGGATCCTG CTCAAAAAAGTGGAAGACTTCACCGGACCCAGAAAGAAGAGCGACCTGGGCTTCATCACCTTCCACATCT CTGCGGATCTGGAGAAGACTTTCGACTGGAACGTCAAGCAGCTCTTCCTTTATCTGTCGGCAGAATATTC CACCAAAAGCAACGCTGTGAACCAGGTGGTCCTCTGGGACAAGATCCTTCTGCGAGGCGAGAACCCCAAG CTGAACCTGAAAGACGTCAAAAGCAAGTATTTTTTCTTTGACGATGGACACGGGCTCAAGGGAAACAGGA ATGTCACTTTGACTCTCTCCTGGCAAGTGATACCGATTGCTGGGATCCTGCCTCTTGTGACTGGATCTGG ACGCGTGTCTGTCCCGTTTCCAGATTCCTATGAAATAGCCACGACTTTTTGAAGAATTCTCTCTGCATTG GAAACGGGGACATCTGTGGACTCAAGAAATCGCATCTCCTCATCATTTCCCTCAAGTCTTGCTGACTTCT CTTTTGTTTTGTTTATTTTAAAACATGCTGGTTTTGTTCTGGGACTGGCTGCACTCATCCTCATGCTTCC CTGAGCCTAACATAGAGACTATATTCATTGATCTTAGTGTGTGTGTGTGACATTGGTTATCACTCTTCCT CTGTATAAGAATTAACATCAAATTTTATAGTTGGAGGGAAAGGCTAATAGAGTTCTGGAACTTTCTTTAT TCTACCAATGAAACAACAAAGAAGTTCACCAGAGTAGGTTATTATTAGACAGTTGGATAATGAGGATTGA AAATAATACACAGTTTTGTCATATTCACTACATTATCCCATGTGATATATAAAATTTGGGGAGAATTTTA AAATTAGATGTTTTGGTATTGTCACAATAACTGTATTTTATTCCTGAGATTCCTTGTAGGTCCACATTGA ATGCCGTTTTCTTATATCTTCTAGGGTGTGTTTGATTACTAGAAAAATTGTGATTTGTTTCGTAAGAGTC TGACATGCAAAATAGTTAAACACGAGTCAGAAATCTTACTGCTTTCCTTCCTTGCCAGCAAATGTGACCA TTTAAATAGTGAACGTATCTTGAGGCTGTAAATGATTCTGCCTCTCTTTAAGTGTGTGTCTTACAGTGGT TTCTCAACAAAGATTGCTGTGCAGTGTATCTTTGTCTTAGCACTGTTGCTGAAGTTTGAAAATGTTTGCA TGTTTAACATGAGATCGTATGTTCTAGTATGTTTAATGCCATTAAATAAATGCTAACTGCTTAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5365902 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5365902 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10997 same embryo
	EMAGE:10996 same embryo
	EMAGE:10999 same embryo
	EMAGE:10998 same embryo
	EMAGE:11001 same embryo
	EurExpress:euxassay_001229 same experiment
	MGI:4822696 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source