EMAGE mouse gene expression spatial database

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EMAGE:11014

Nfs1 nitrogen fixation gene 1 (S. cerevisiae) ( MGI:1316706)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003413_01	euxassay_003413_02	euxassay_003413_03	euxassay_003413_04	euxassay_003413_05

euxassay_003413_06	euxassay_003413_07	euxassay_003413_08	euxassay_003413_09	euxassay_003413_10

euxassay_003413_11	euxassay_003413_12	euxassay_003413_13	euxassay_003413_14	euxassay_003413_15

euxassay_003413_16	euxassay_003413_17	euxassay_003413_18	euxassay_003413_19	euxassay_003413_20

euxassay_003413_21	euxassay_003413_22	euxassay_003413_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11014_wholemount_strong.wlz

11014_wholemount_moderate.wlz

11014_wholemount_weak.wlz

11014_wholemount_possible.wlz

11014_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11014_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6823
Entity Detected:	Nfs1, nitrogen fixation gene 1 (S. cerevisiae) ( MGI:1316706)
Sequence:	sense strand is shown >T6823 GCTGAGGACTTGCAAGCGCCGCGGATGGTGGGCTCTGTGGCTGGGAACATGCTGCTGCGAGCCGCTTGGA GGCGGGCGTCGTTGGCGGCTACCTCCTTGGCCCTGGGAAGGTCCTCGGTGCCCACCCGGGGACTGCGCCT GCGCGTTGTAGATCATGGCCCCCATTCGCCTGTTCACTCAGAGGCAGAGGCAGTGCTGCGACCTCTCTAC ATGGACGTACAGGCCACCACTCCTCTGGATCCCAGAGTGCTTGATGCCATGCTCCCATACCTTGTCAACT ACTATGGGAACCCTCATTCTCGGACTCATGCATATGGCTGGGAGAGCGAGGCAGCCATGGAACGTGCTCG CCAGCAAGTAGCATCTCTGATTGGAGCTGATCCTCGGGAGATCATTTTCACTAGTGGAGCTACTGAGTCC AACAACATAGCAATTAAGGGAGTGGCCAGGTTCTACAGGTCAAGGAAAAAACACTTGGTCACCACCCAGA CAGAACACAAATGTGTGCTGGATTCCTGCCGCTCCCTGGAAGCTGAGGGCTTTCGGGTCACTTACCTCCC TGTGCAGAAGAGCGGGATCATTGACTTAAAGGAACTAGAGGCTGCCATCCAGCCAGACACCAGCCTGGTC TCCGTTATGACTGTGAACAATGAAATTGGAGTAAAGCAACCGATTGCAGAAATAGGGCAGATTTGTAGTT CCAGAAAGGTGTACTTCCACACTGATGCCGCCCAAGCGGTTGGGAAAATCCCACTTGACGTCAATGACAT GAAGATTGATCTCATGAGCATCAGCGGTCACAAACTCTATGGCCCTAAAGGGGTTGGTGCTATCTATATC CGCCGTAGGCCCCGGGTACGTGTGGAGGCCCTACAGAGTGGAGGCGGGCAGGAGCGGGGTATGCGGTCTG GGACAGTGCCCACACCCTTGGTGGTGGGCCTGGGAGCTGCATGTGAGTTGGCACAACAAGAGATGGAATA CGACCATAAGCGGATCTCAAAGTTAGCAGAGCGACTGGTACAGAAGATAATGAAGAACCTTCCAGATGTG GTAATGAACGGGGACCCGAAGCAGCACTACCCTGGCTGTATCAACCTCTCCTTCGCGTATGTGGAAGGAG AGAGCCTGCTGATGGCCCTCAAGGATGTGGCCTTGTCCTCAGGGAGTGCCTGCACTTCTGCGTCATTGGA GCCCTCTTACGTCCTCAGAGCAATTGGTACCGATGAGGATTTAGCACACTCCTCTATCAGGTTTGGCATC GGCCGCTTCACTACAGAAGAGGAGGTGGACTACACGGCGGAGAAGTGCATCCACCACGTGAAGCGCCTTC GAGAAATGAGTCCTCTCTGGGAGATGGTCCAGGATGGCATTGACCTCAAGAGCATCAAGTGGACCCAGCA TTAGAGGAGTGGGCTGTGACTTTGTGCTGGCCTGGCTGTTCTGGCCTCACTGATCTGTGCACAGCCAGGA ACCTCACTGCCTGACGTGGTCCATGCTCAAAGCAGATGGCAAGCTCTGCAGTGACTTCACCACAGAATGT TCTCTAGTGAAAGCTGAGGCTAGCTTTCCTAGCCTCGGCTCCTGTGTAGGCATGTATTCCGTAATCTTCC TCAGGAGTTCTTCTCTTGCCTGGCGTGGCCATTCTTATCTGAGGGCCTGGAGGCACTGCCTTGTTACTGC CACAGGAGGACCTACCTGTGCAGAGATGGAAGAACTGGAAGAAGGCTCTAGGTTCAAAGACTGCTTCTCT GTGAATCATTACTCTAGCTGCTCAGCTGAACTGGCGCCATTAGGGACTGGCATAAACAAACTTTATTTTA ACCTTTTTTGTGGCTTTGAAAGACATCTCTTTGTATCGACTTCAGCTGAAGAACACCAAGTTGTATTAAA GTCTTGTTTCCTTTGGGTTCTCATCTTCTTTCCTCTCTTTTGACTTAATGTAAGTAGAAATATCCATGAC CCCAATCATTATTCTAATAATTTACTTAATTCATTAAACTTAATTCAATAAATTCCCAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:3711352 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3711352 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11013 same embryo
	EMAGE:11017 same embryo
	EMAGE:11016 same embryo
	EMAGE:11015 same embryo
	EurExpress:euxassay_003413 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source