EMAGE mouse gene expression spatial database

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EMAGE:11020

Hmga1-rs1 high mobility group AT-hook I, related sequence 1 ( MGI:96161)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003457. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003457_01	euxassay_003457_02	euxassay_003457_03	euxassay_003457_04

euxassay_003457_05	euxassay_003457_06	euxassay_003457_07	euxassay_003457_08	euxassay_003457_09

euxassay_003457_10	euxassay_003457_11	euxassay_003457_12	euxassay_003457_13	euxassay_003457_14

euxassay_003457_15	euxassay_003457_16	euxassay_003457_17	euxassay_003457_18	euxassay_003457_19

euxassay_003457_20	euxassay_003457_21	euxassay_003457_22	euxassay_003457_23	euxassay_003457_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11020_wholemount_strong.wlz

11020_wholemount_moderate.wlz

11020_wholemount_weak.wlz

11020_wholemount_possible.wlz

11020_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11020_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 22 23 24

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

spleen primordium

moderate

regional

moderate expression: see section 05 23 24

thymus primordium

strong

homogeneous

strong expression: see section 12 13 14 15 16

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09 10 16 17 18 19 20

pancreas

strong

regional

strong expression: see section 10 11 12 13 17 moderate expression: see section 18 19 20

thyroid gland

strong

regional

strong expression: see section 10 11 12 15 16

pituitary gland

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 11 15

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

strong

regional

strong expression: see section 05 06 07 08 09 19 21 24 moderate expression: see section 03 04 22 23

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 10

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 08 09 10 11 18 19 20 weak expression: see section 05 06 07 12 15 16 17 21 22

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 16 17 18 19 20 21

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 weak expression: see section 16

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 23 24

neural retina

strong

homogeneous

strong expression: see section 01 02 moderate expression: see section 03 22 23 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 10 11 12 14 15 16 17 18 19 moderate expression: see section 09

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 11 12 13 15 16

esophagus

moderate

regional

moderate expression: see section 12 13 14

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11

hindgut

strong

regional

strong expression: see section 15 16 17 18

rectum

weak

regional

weak expression: see section 15 16

midgut

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 12 15 16 17

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

bladder

moderate

regional

moderate expression: see section 14 15 16

renal cortex

moderate

regional

moderate expression: see section 07 08 09 10 11 18 19 20 21 weak expression: see section 22

maturing glomerular tuft

moderate

regional

moderate expression: see section 07 08 09 10 11 18 19 20 21 weak expression: see section 22

testis

strong

regional

strong expression: see section 06 07 08 09 moderate expression: see section 21 22 23 24

urethra of male

moderate

regional

moderate expression: see section 15 16

thyroid cartilage

moderate

regional

moderate expression: see section 12 13 14

left lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11

right lung

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20 21 22 23 24

trachea

moderate

regional

moderate expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6251
Entity Detected:	Hmga1-rs1, high mobility group AT-hook I, related sequence 1 ( MGI:96161)
Sequence:	sense strand is shown >T6251 CCGAGTCGGGGCTATTTCTGGTGTTGGCGCGGCTCCAAGAAGGCACTCACTGTGTGCTGCCCCACACATA GAGAAGGAGAATGAGCGAGTCGGGCTCAAAGTCCAGCCAGCCCCTGGCCTCCAAACAGGAAAAGGATGGG ACTGAGAAGCGAGGCCGGGGCAGGCCACGCAAGCAGCCTCCGAAAGAGCCCAGTGAAGTGCCAACTCCGA AGAGACCTCGGGGCCGACCAAAGGGAAGCAAGAATAAGGGCGCCGCCAAGACCCGGAAAGTCACCACAGC TCCAGGGAGGAAACCAAGGGGCAGACCCAAGAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCCCAGGAG TCCTCTGAGGAGGAGCAGTGATCACCACTCGCAGTGCCGCCTGCTCCATAGCCACTGAGGAGCAGCTGTC CCCTGGGACTGGAAGCCCTGCTCCCAGGCCCCTCCCCGCCCCCACTGCACACTACCGCACCAGGCACCAG CTGCAGGCTCTGCTCCGCAGCACCCAAGGGAGCAGGATTCGGCTCAGTCCCTGTGCCCTCCTCCCGAACA CACCTGCCCCATCCTGACAAGGCTAACTTCCCATTTAGCCGCAGCCCGGCCTGCTGCGCACCTCCCTCAG AGCCGTGGCTCTCTGGGCGCTTTGTTTTGCGGGTCCCTTCTGCCCTGCTGTTCCTGGCTTCCCCGTAGAG GGTCCTGGGAGCATTCCCTGGCCTTAAGGGGCCCCGGCCCCATCTCACTCTGACAAGGCCCAGTGGCTGA CCTAGCAGCAGCAGTGTGGTCAGGGCAGGGGATGCTTAGATCCCAGCATGTCCCCACCAAAGTATCACCG GGTGGGTTTCTCAGCCCCTCCTGCCCCACCCTACAGAGATCATGCACTGGGTTGGCTGAGCCCCTTGACA ACAGGAAGTAGGATGATGTTGGCTCCACCCACTTTAATGTTCTCACCTGAGACTAGACAGATGGGGTTAC CGCCCATCTCCAGAGTAAGGGCCTGTTCTCGCCTCCACCAAAGGCAAACGGGCACCAACACGCCTCCCCC AGTCCACTCTAGGCTGTTCCTTCCTCCCGCAGGGCACTAAGGAGCTAGCCTTGCCGCCCCCTCCTTTTCG CTCCTCCCTGCCCCCCATGGTTCTGGTTCCATCTTCTCCTCTGTTCACAAACTACCTCTGGACGGTTGTG TTGTTTTTTTTTTGTTCCATGTTCATTCTTAGATACCCACTATTGCTGCTGCTACCAGCGTCAGATGTTC ATCCTCCTGAATTTGCCTGTATTCTCTCCCCCACACAATACACTCGAGGGGAAGGGGGGCTGGGGCGCAG CAGACTGGTTACCTACCCCACACTAAGGAAGATGGGGCCCCACCCCGTGGATGCTGCAGCAGAGTGAGGA TGGGGGCCCACTTTGATGGGAAAAGGGTGTAGGGCCACCTCCCCTTGTTCTGTGGGGGAGGGGTGGCCAG TATTTGTCCCAGCCTGGGGCTTCCTCCCTGGTTTCTTATTTGCAGTTACTTGAATAAAAAACAAATAGCC AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3500572 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3500572 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11019 same assay
	EMAGE:11023 same embryo
	EMAGE:11022 same embryo
	EMAGE:11021 same embryo
	EurExpress:euxassay_003457 same experiment
	EMAGE:11018 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source