EMAGE mouse gene expression spatial database

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EMAGE:11022

Ptdss1 phosphatidylserine synthase 1 ( MGI:1276575)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003429. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003429_01	euxassay_003429_02	euxassay_003429_03	euxassay_003429_04

euxassay_003429_05	euxassay_003429_06	euxassay_003429_07	euxassay_003429_08	euxassay_003429_09

euxassay_003429_10	euxassay_003429_11	euxassay_003429_12	euxassay_003429_13	euxassay_003429_14

euxassay_003429_15	euxassay_003429_16	euxassay_003429_17	euxassay_003429_18	euxassay_003429_19

euxassay_003429_20	euxassay_003429_21	euxassay_003429_22	euxassay_003429_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11022_wholemount_strong.wlz

11022_wholemount_moderate.wlz

11022_wholemount_weak.wlz

11022_wholemount_possible.wlz

11022_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11022_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 05 06 07 20 21 weak expression: see section 08 09

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 07 08 09 15 16

pons mantle layer

weak

regional

weak expression: see section 06 17

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 06 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 08 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 08 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 17 18

ventral grey horn

moderate

regional

moderate expression: see section 10 12 13 14 15 16 weak expression: see section 11

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6253
Entity Detected:	Ptdss1, phosphatidylserine synthase 1 ( MGI:1276575)
Sequence:	sense strand is shown >T6253 GTTTGCAGGCCCTGGAGCTGGTCGCCGCGTTCCCAGGATCAGTCACTCCGCCGTAATGTGGGGAGGGTGA GAGCTCTACGATGTCGCCCTGCTAGGCTGCTTCTTCAGGCTGCCTTCTCCCCCTGCTCCGGGACGCGGTG GTGGCGCCGCGACCCCGCCCTCGCGCGCGGGTCCTTCCCTGGGCTAGCACCCTTCTCAGGGTGCCAGACC CCGCCCCAGCGTCAGGCACCTTCGCTGCTGCGGTCCGTCGCTGCCGGGGTCCCAGCCTTCTAGGGCCGGG GCCTCCGGGGCCGCGGCAGGACGGGGAGAAGAACTATGGCGTCCTGCGTGGGGAGCAGGACTCTGAGCAA GGATGATGTGAACTACAGGATGCATTTCCGCATGATCAACGAGCAGCAAGTGGAGGACATCACCATAGAC TTCTTCTACCGGCCGCACACCATCACCCTGCTCAGCTTCACCATCATCAGCCTCATGTACTTCGCCTTCA CCAGGGATGACTCTGTTCCGGAAGACAACATCTGGAGAGGCATCCTGTCTGTTATCTTCTTCTTTCTCAT CATCAGCGTGTTAGCATTCCCCAATGGTCCATTTACTCGACCTCATCCAGCCTTATGGCGAATGGTTTTT GGTCTCAGTGTGTTGTATTTCCTCTTCTTGGTATTCCTCCTTTTCCTGAACTTCGAGCAGGTTAAATCCC TGATGTATTGGCTGGATCCCAATCTTCGATATGCTACACGAGAAGCGGACATCATGGAGTATGCTGTGAA CTGTCATGTGATAACCTGGGAGAGAATCGTTAGTCACTTTGATATATTTGCATTTGGCCATTTCTGGGGC TGGGCCATGAAGGCCTTGTTGATCCGTAGTTATGGGCTTTGCTGGACAATCAGTATCACCTGGGAGCTGA CAGAGCTTTTCTTCATGCATCTCCTGCCCAATTTTGCTGAGTGCTGGTGGGACCAAGTCATTCTGGACAT CCTGTTGTGCAATGGTGGTGGAATCTGGCTGGGCATGGTCGTTTGCCGGTTTCTGGAGATGAGAACTTAC CACTGGGCAAGCTTCAAGGACATCCACACCACCACAGGGAAGATCAAGCGAGCTGTGCTGCAGTTCACCC CTGCTAGCTGGACTTACGTCAGGTGGTTTGATCCCAAGTCTTCCTTTCAGAGAGTGGCTGGTATATACCT CTTCATGATCATATGGCAGTTGACTGAGTTGAATACCTTCTTCTTGAAGCACATCTTTGTGTTCCAAGCC AGCCATCCCTTAAGCTGGGGTAGAATCCTCTTCATCGGCTGCATTACGGCTCCCACAGTGAGACAGTACT ATGCCTACCTCACGGACACACAGTGCAAGCGTGTAGGGACACAGTGCTGGGTGTTTGGGGTCATTGGTTT CCTGGAAGCTATTGTTTGCATAAAATTTGGACAAGATCTCTTTTCTAAGACCCAGATACTCTATGTGATG CTTTGGCTTCTTTGTGTGGCCTTCACCACGTTCCTGTGTCTGTACGGCATGGTTTGGTATGCAGAGCACT ATGGTCACCGGGAAAAGACCTATTCAGAGTGTGAAGATGGCACTTACAGTCCAGAGATCTCCTGGCATCA TGGGAAAGGCTCCAAAGGTTCTGAAGACAGCCCACCGAAGCATTCAAGCAACCACGAAAGCCATTCTTCC AGAAGAAGGAACCGGCATTCTAAGTCCAAAGTCACTAATGGAGTTGGAAAGAAATGAAGGAAGCTAGTTA ATGTCAGATGTTCCAGAGTGCCTAGACCCGAGAGGAAAACAGCATATTTGGACCTTCCTATGCAGAGATC ATAATGTACGGAAAGAACCGAGGGAGCTTAGGGGATCGTGACTTGAGAGTGTAAGTCTTGTCTCCCATAG ACCTGGACACACACTAGGCTGACCATGGCTCGGGGTCCTTTGCCAACTGGAAGAGTCTCTAAACTTCAGC GTATGGCCTGCATTCTCTGGTAGCAATACAGTGTGCTGGAAGGACAGGATAGCTGTTTATTCCCAGCTGC CAGCAGTGGCTCTCTGCTGGTGTGTCTTGTGGATGTTGTATAAATGTCTTCATTCAGATGTTGACTTAAC TAACTGGCTAATGAACTGGTCGCTTGGTTTTATTTTTATGAGTCTGCCTGTCCATTTGATTGATTGTTTA AGTTCAGAGAGTCTGCTTTTACTTCATGTTTTCGAGTTAAATAGCTTCAAAGCTTCCAGAATCCACTGCT GAAATTGGGGGAGGGTTCCTTCTAGAGAAACAAAGGGTGGAAGGAGCTTTGGTGTCCCAGCTAAGCATAC TCAGTCCCCAGCCACATGGCAGATGACCCCAGCCGGAGTGAAGAGGCTGGCTGCTGGCACTGGAGCCTCG GCTTCAGATTAGAACTGTTTTGTTCCCTTCCAGACACTGTCCAGACTGAGTGCACAAGAACGACCTTGCG AGCGGATCTGGGACAGTGGGGCTCCCTGCCAGGGTCCTTTTCCATGTGCTTGGTTTTTCCTCTGTGATGT TTTGCCTACTCTGTCTTTCTGGCTCATGTGAGGTGCTAGTGGGTATTAATTAACTTTCATCTGTGCCATG TTCTACTAAAACATTCTCCCTGTCTACCCTGGATCCTTGTTTTAAATATATCAGTTCACATTAAACAGCT CATCAATGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3501141 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3501141 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11023 same embryo
	EMAGE:11021 same embryo
	EMAGE:11019 same embryo
	EMAGE:11020 same embryo
	EMAGE:11018 same embryo
	EurExpress:euxassay_003429 same experiment
	MGI:4827500 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source