EMAGE mouse gene expression spatial database

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EMAGE:11025

Anxa6 annexin A6 ( MGI:88255)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003434. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003434_01	euxassay_003434_02	euxassay_003434_03	euxassay_003434_04

euxassay_003434_05	euxassay_003434_06	euxassay_003434_07	euxassay_003434_08	euxassay_003434_09

euxassay_003434_10	euxassay_003434_11	euxassay_003434_12	euxassay_003434_13	euxassay_003434_14

euxassay_003434_15	euxassay_003434_16	euxassay_003434_17	euxassay_003434_18	euxassay_003434_19

euxassay_003434_20	euxassay_003434_21	euxassay_003434_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11025_wholemount_strong.wlz

11025_wholemount_moderate.wlz

11025_wholemount_weak.wlz

11025_wholemount_possible.wlz

11025_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11025_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5922
Entity Detected:	Anxa6, annexin A6 ( MGI:88255)
Sequence:	sense strand is shown >T5922 CGGGCTTGGAGCTGCGTCTGTCCGTACGCCCTGATCTGAGGCCTCCCTAGCGCGTGGTTTCTGCTGCAGA ACCTGAGACCATGGCCAAAATAGCACAGGGTGCCATGTACCGAGGCTCTGTCCACGACTTCCCAGAGTTT GACGCAAATCAGGATGCTGAGGCCTTGTACACAGCCATGAAGGGCTTCGGCAGTGACAAGGAGTCCATAC TGGAGCTGATCACCTCCCGCAGCAACAAGCAGAGGCAGGAGATCTGCCAGAGTTACAAGTCCCTGTATGG CAAGGACCTCATCGAAGACTTGAAGTATGAGTTGACGGGGAAGTTCGAGCGGCTGATAGTGAACCTGATG AGGCCACTTGCCTATTGTGACGCCAAAGAGATTAAAGACGCCATCTCGGGCATTGGCACAGATGAGAAGT GCCTCATTGAAATTTTGGCTTCCCGGACCAATGAACAGATGCACCAGCTGGTGGCCGCATACAAAGACGC CTATGAGCGAGACCTGGAATCTGACATCATTGGAGACACTTCCGGCCACTTCCAGAAGATGCTGGTGGTA CTGCTCCAGGGAACCCGGGAGAATGACGATGTTGTGAGCGAGGATTTGGTCCAGCAGGATGTCCAGGACC TGTATGAGGCAGGGGAACTGAAATGGGGAACAGATGAGGCCCAGTTCATCTACATCTTGGGAAACCGTAG CAAACAGCACCTACGACTGGTGTTTGATGAGTATCTGAAGACCACAGGGAAGCCCATCGAAGCCAGTATC AGAGGGGAGCTGTCTGGAGACTTTGAGAAGCTGATGTTGGCCGTGGTGAAGTGCATCCGAAGCACCCCGG AGTATTTTGCAGAAAGGCTGTTCAAAGCCATGAAGGGCCTAGGGACCCGAGACAACACTCTGATCCGCAT CATGGTCTCCAGGAGCGAGCTGGATATGCTTGACATCCGGGAGATCTTCCGGACCAAGTATGAGAAGTCA CTCTACAGCATGATCAAGAATGATACTTCCGGTGAATACAAGAAGGCTCTGCTGAAGCTGTGTGGAGGAG ATGATGATGCCGCTGGCCAGTTCTTCCCGGAGGCAGCACAGGTGGCCTATCAGATGTGGGAACTTAGTGC AGTGTCCCGAGTCGAGCTGAAGGGTACTGTGTGTGCAGCCAATGATTTCAACCCTGACGCCGATGCCAAG GCCCTGCGGAAAGCCATGAAGGGAATTGGAACTGATGAAGCCACCATCATCGACATCGTCACCCACCGGA GCAACGCCCAGCGGCAGCAGATCCGGCAGACCTTCAAATCTCACTTTGGCCGGGATTTAATGGCTGACCT GAAGTCAGAGATCTCGGGAGACCTGGCAAGGCTGATTCTGGGGCTCATGATGCCACCTGCCCATTACGAT GCTAAGCAGCTGAAGAAAGCTATGGAGGGAGCCGGCACAGATGAAAAGACTCTCATAGAAATCCTGGCCA CCCGGACCAATGCTGAAATCCGGGCCATCAACGAGGCCTACAAGGAGGATTATCACAAGTCCCTGGAGGA TGCCTTGAGCTCAGACACATCTGGCCACTTCAGAAGGATTCTCATTTCTCTGGCCACGGGAAATCGAGAG GAAGGAGGAGAAAACCGGGACCAGGCCCAGGAAGATGCCCAGGAAATAGCAGACACCCCCAGCGGAGACA AAACTTCCTTGGAGACACGCTTCATGACCGTCCTGTGCACCCGTAGCTATCCCCACCTGCGCAGAGTCTT CCAGGAGTTCATCAAGAAGACCAACTATGACATAGAGCATGTCATCAAGAAGGAGATGTCTGGGGACGTC AAGGACGCATTTGTGGCCATCGTTCAGAGTGTCAAGAACAAGCCTCTCTTCTTTGCTGATATACTGTACA AGTCCATGAAGGGTGCTGGCACAGATGAGAAGACCCTCACCAGGGTGATGGTGTCTCGGAGTGAGATAGA TCTGCTCAACATCCGGAGGGAATTCATTGAGAAATATGACAAGTCTCTACACCAAGCCATTGAGGGTGAC ACCTCTGGAGACTTCATGAAGGCTTTGCTTGCTCTGTGTGGCGGAGAGGACTAAAGCTCCTGACCTCAGA GGGCCCCCTCTACCAAGCAGTGGCCAACTGCACCAGCTGCCGCCGTGACCTGACCAAGCCATTGCTCCAG CTTCAGGATCTAAGGAAGACGCAAGGGGTGGGGTTGGACTGTCCATCCAGCTGGGGCACTCTCTAGCCAT TGAGGGTCCCAGGCGGTCCCTTCTGTTATCCCTCCCGCTCATGGCTAAGGGGCTGGTGAACCACGGCCAT ATACTGCATTCAACTCTGATCCCCTTCTACCTACTCCTCCGCCCGCCCTGGTTTCCATCCCTCCACATAA CCCTGCCTGACTTGAGCTTTGTCACATCTCAAGACATACCAAACCTCTGTCTATGAAATGAGTGATGTGT GTGCTTTAAAGGGCGGGCTGTGGTGCCTGGTGCCAGGCAAGCATTCTGGGATAATAGAGATGACAGATTA ATTTGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3482035 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3482035 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11024 same embryo
	EMAGE:11026 same embryo
	EurExpress:euxassay_003434 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source