EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11026

Anapc7 anaphase promoting complex subunit 7 ( MGI:1929711)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003522. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003522_01	euxassay_003522_02	euxassay_003522_03	euxassay_003522_04

euxassay_003522_05	euxassay_003522_06	euxassay_003522_07	euxassay_003522_08	euxassay_003522_09

euxassay_003522_10	euxassay_003522_11	euxassay_003522_12	euxassay_003522_13	euxassay_003522_14

euxassay_003522_15	euxassay_003522_16	euxassay_003522_17	euxassay_003522_18	euxassay_003522_19

euxassay_003522_20	euxassay_003522_21	euxassay_003522_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11026_wholemount_strong.wlz

11026_wholemount_moderate.wlz

11026_wholemount_weak.wlz

11026_wholemount_possible.wlz

11026_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11026_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5923
Entity Detected:	Anapc7, anaphase promoting complex subunit 7 ( MGI:1929711)
Sequence:	sense strand is shown >T5923 CCGAGCCTCTGCTCTCGGTTCCCGGGAGTTCTGTCCGCCGCTGCCGGTGCCGCGGTGTCGCCGGGGGCCA CGGTGTTGCCGGGCAGGATGAGTGTGATAGACCACGTGCGGGACATGGCGGCTGCGGGACTGCACTCCAA CGTGCGGCTCCTCAGCAGCCTGCTGCTCACCATGAGTAACAACAACCCTGAGCTGTTTTCCCCGTCGCAG AAGTACCAGCTCTTGGTGTATCATGCAGACTCTCTGTTTCACGATAAGGAGTACCGCAATGCTGTGAGTA AGTACGCCATGGCTTTACAGCAGAAGAAAGCCTTAAGTAAGACTTCCAAAGTGCGGCCTTCCACTGGGAA TTCTGCATCTACTCCACAGAGTCAGTGTCTTCCATCTGAAATTGAAGTGAAATACAAAATGGCTGAGTGT TACACAATGCTGAAGCTAGACAAGGATGCCATTGCTGTACTCGATGGGATCCCTTCAAGACAAAGAACTC CCAAAATAAACATGATGCTGGCAAACCTGTACAAGAAGGCTGGTCAGGAGCGCCCATCAGTCACAAGCTA TAAGGAGGTCCTGCGGCAGTGCCCTTTGGCCCTTGACGCCATTCTAGGTTTGTTATCCCTGTCTGTGAAA GGCGCAGAGGTGGCATCAATGACGATGAATGTGATCCAGACTGTGCCCAACTTGGACTGGCTGTCCGTGT GGATTAAAGCGTATGCTTTTGTGCACACCGGTGACAACTCGAGAGCAATCAACACCATCTGTTCACTAGA GAAAAAATCATTACTGCGAGACAACGTGGACCTGCTGGGGAGCCTGGCCGACCTGTACTTCAGAGCAGGG GACAGCAAGAACTCTGTCCTCAAGTTTGAGCAGGCCCAGATGCTGGATCCCTACTTGATAAGAGGGATGG ATGTTTACGGCTACCTCCTGGCACGAGAAGGGCGGCTGGAGGATGTGGAGAACCTTGGCTGCCGCCTCTT CAACATTTCTGATCAGCATGCAGAGCCCTGGGTGGTCTCCGGATGCCACAGCTTCTATAGCAAGCGCTAC TCTCGGGCCCTGTACTTAGGTGCCAAGGCCATCCAGCTGAACAGCAACAGCGTCCAGGCTCTGTTACTCA AGGGGGCGGCGCTTAGGAACATGGGGAGGGTCCAGGAAGCCATCATACACTTTCGGGAGGCCATCAGGCT TGCGCCTTGTCGCTTGGACTGTTACGAAGGTCTCATAGAGTGTTACTTAGCTTCTAACAGCATCCGAGAA GCAATGGTGATGGCCAACAATGTTTACAAAACTCTGGGTGCAAACGCTCAGACGCTTACGCTGTTAGCCA CCGTGTGTCTGGAAGATCCAGTGACCCAGGAGAAAGCCAAAACTCTGTTAGATAAAGCCCTGGCGCAGAG GCCGGACTACGTCAAGGCCGTGGTGAAGAAAGCAGAGCTGCTCAGCAGGGAACAGAAATATGAAGATGGA ATTGCTCTGCTGAGAAATGCGCTGGCCAACCAGAGTGACTGTGTCCTGCATCGGATCCTGGGGGACTTCC TAGTAGCTGTGAACGAGTACCAGGAAGCGATGGACCAGTACAGTATAGCACTGAGTTTGGACCCCAATGA CCAGAAGTCTCTCGAGGGGATGCAGAAGATGGAGAAGGAGGAGAGTCCCACGGATGCCACTCAGGAGGAA GATGTGGACGACATGGAGGGGAGTGGGGAAGAAGGGGACCTGGAGGGCAGCGACAGTGAGGCAGCCCAGT GGGCAGACCAGGAGCAGTGGTTCGGCATGCAGTGAAGGCACAGTGGCAGCGCCTGGTGTTCCGAGAGTGA CAGATGCTGTCGGAAGTGTGCTCACAGCAGGGCTCCCTGCCTGTTTCCAAGTAGTAGTCGGGACTGGTGT CTACCTGTCTGCTTTGCTGAGGACTGAGCGAGTTCCAAGCTGCAGACTCACCATTATGGATGGTTCTGAC GTACCCAGTGGACGATGCCTTTTTCTCTTTATGACGGGTACACAGAACTGAGCCATCCTCTTAAGGGCAC ATGCACCTGGGTGGTCCTGCCAGAGGGAATCTGGTTCCTGGGAGTCAGGGTCCTCCCTGCCACACTCCCT ATAGTCATGATGGTCCGGATGCGGGTACCGTGCTTCACGGACTGCAGAGCAGCACTTGGCCCCAGTGGGC AGATCTGAGGAGCAGATGATGGCCACACACAGGACAGGCTCACCTAATAAACTGTTCAGTGTGGTGGCAC AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3482261 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3482261 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11025 same embryo
	EMAGE:11024 same embryo
	EurExpress:euxassay_003522 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source