EMAGE mouse gene expression spatial database

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EMAGE:11071

Gfi1b growth factor independent 1B ( MGI:1276578)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006575. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006575_01	euxassay_006575_02	euxassay_006575_03	euxassay_006575_04

euxassay_006575_05	euxassay_006575_06	euxassay_006575_07	euxassay_006575_08	euxassay_006575_09

euxassay_006575_10	euxassay_006575_11	euxassay_006575_12	euxassay_006575_13	euxassay_006575_14

euxassay_006575_15	euxassay_006575_16	euxassay_006575_17	euxassay_006575_18	euxassay_006575_19

euxassay_006575_20	euxassay_006575_21	euxassay_006575_22	euxassay_006575_23	euxassay_006575_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11071_wholemount_strong.wlz

11071_wholemount_moderate.wlz

11071_wholemount_weak.wlz

11071_wholemount_possible.wlz

11071_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11071_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8083
Entity Detected:	Gfi1b, growth factor independent 1B ( MGI:1276578)
Sequence:	sense strand is shown >T8083 AAGAGGTAATCCGACTCATAACGTTGACCGAGCCGAGAGCAGTCCCCAGGGACAGTGTGGAGGTTCGTGG CTCTCGGGCAGAACTCAGAAGAGGGACAGCTCCCTGACGGTGTGGCGTGCACGCAGAAAAATGCCACGGT CCTTTCTAGTGAAGAGTAAGAAGGCACACACTTACCACCAGCCCCGGGCACAGGGTGATGAGCTGGTCTG GCCTCCTGCTGTAATTCCTGTGGCAAAAGAGCATAGCCAGAGTGCCAGCCCTCTTCTCAGCACACCGCTT CCAAGCCAGACCTTGGACTGGAACACAATCAAACAGGAGCGGGAGATGTTGCTGAACCAGAGCCTTCCCA AGATGGCCTCAGCCCCAGAGGGGCCTCTCGTGACACCCCAACCCCAGGATGGGGAATCACCACTCTCTGA GTCACCCCCTTTCTACAAGCCCAGCTTCTCCTGGGATACCTTGGCCTCCTCCTACAGCCACAGCTACACA CAGACCCCCTCCACCATGCAGTCCGCCTTCCTGGAGCGCTCCGTGAGGCTGTACGGCAGCCCCCTCGTGC CCAGCACAGAGTCTCCCTTGGACTTCCGCCTCCGCTACTCTCCAGGCATGGACACTTACCACTGTGTCAA GTGCAACAAGGTGTTCTCCACCCCTCATGGGCTAGAAGTGCATGTCCGCCGCTCTCACAGCGGAACCCGG CCCTTTGCCTGTGATGTCTGTGGCAAAACCTTTGGCCACGCTGTGAGCTTGGAGCAGCATACTCACGTCC ACTCACAGGAGCGAAGCTTCGAGTGCCGGATGTGTGGCAAAGCCTTCAAGCGTTCATCCACCCTGTCCAC CCACCTGCTCATCCACTCGGACACTCGGCCCTACCCCTGCCAGTTCTGTGGGAAGCGCTTCCACCAGAAG TCGGACATGAAGAAACACACCTACATCCACACAGGTGAGAAGCCCCACAAGTGCCAGGTGTGTGGGAAAG CCTTCAGCCAGAGCTCCAACCTCATCACCCACAGCCGCAAGCACACAGGCTTCAAGCCGTTCAGCTGTGA GCTGTGCACCAAGGGCTTCCAGCGCAAGGTGGACCTGCGACGTCACCGTGAGAGTCAACACAATCTCAAG TGAGACGGTTGGCCGCCTGCTTTAGTGTGTCTCGCCTGAAGGCCAGCCTCTCCTTTCCAATCCTGATCCC AGTCCTCCTGGAAGCAGCATTGCCCATGAGCCTCTCTGCTTCTTTTGAGACTGGATGACCTCAACCAAGC CACACTCCTCCTCTGACCACAGCTGATGTGGGAGTCAGTTCGGGCCTTTCTGAACTGAGGCAGTCACAGA CATGCTCTGGTCTCATTCAATGCCAGAGCACAGACAGCTACAAAGCCCTCGTGGACATGGCAAAACCAGG CAAGAGCCAGGATCCACAGAGAAACCCTCTTGCTTGCTTCTCTGGGTCTAGTGACTGAATTTTTAGGGAT TCTCTTTTTGGCTGCCACAGTCAGAGTTGTCCCCTGGCCTAGAGGTCTGGCGCCCCTCTGCTTCGGCCAG ATGTGCTCTCTGTCCCCCACCTCTCACCACATCCATCCGCCCACTGGGAGTCAGGCTCCTGAGATGAGCC ACGAGGCTATGAAATCAGCCATGATAGTAGAAGCCTGCTCCTATCAGTTGATGTAAATGTAAATAAAGGT GGGTTTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30039774 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30039774 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11070 same embryo
	EMAGE:11069 same embryo
	EMAGE:11072 same embryo
	EMAGE:11068 same embryo
	EurExpress:euxassay_006575 same experiment
	MGI:4825043 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source