EMAGE mouse gene expression spatial database

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EMAGE:11085

Klhl26 kelch-like 26 (Drosophila) ( MGI:2443079)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006489. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006489_01	euxassay_006489_02	euxassay_006489_03	euxassay_006489_04

euxassay_006489_05	euxassay_006489_06	euxassay_006489_07	euxassay_006489_08	euxassay_006489_09

euxassay_006489_10	euxassay_006489_11	euxassay_006489_12	euxassay_006489_13	euxassay_006489_14

euxassay_006489_15	euxassay_006489_16	euxassay_006489_17	euxassay_006489_18	euxassay_006489_19

euxassay_006489_20	euxassay_006489_21	euxassay_006489_22	euxassay_006489_23	euxassay_006489_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11085_wholemount_strong.wlz

11085_wholemount_moderate.wlz

11085_wholemount_weak.wlz

11085_wholemount_possible.wlz

11085_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11085_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6591
Entity Detected:	Klhl26, kelch-like 26 (Drosophila) ( MGI:2443079)
Sequence:	sense strand is shown >T6591 CGCGCGGCGGAAGATGGCGGAGTCCGGTGGCAGCAGCGGCTCTAGTCAGAGCCCCGAGCGCCCGAGCAGC TTGGCCGACAGGAATGGGGCACTTAAGTGCACCTTCTCTGCAGCTGGCCACAGCACTGGCCTCCTGCAGG GCCTGGCTGCCCTCCGTGCACAGGGCCAGCTGCTCGATGTGGTCCTCACTGTCAACAGTGAAGCCTTTCA TGCGCACAAGGTGGTCTTGGCGGCCTGCAGTGACTACTTTAGGGCCATGTTCACTGGCGGCATGCGTGAG GCCAACCAGGCTGTCATCCAGCTGCAGGGTGTGTCTGCACGTGGCCTGCGTCACATCATCGATTTTGCCT ACAGTGCTGAGGTGACACTGGACCTAGACTGTGTGCAGGACGTGCTGGGTGCTGCCGTGTTTCTGCAGAT GCTGCCGGTGGTGGAGCTGTGCGAGGACTTCCTGAAAGCTGCCATGAGCGTAGAGACGTGCCTGCACATT GGCCAGATGGCTACCACCTTCAGCCTGACATCACTGCGTGAGTCAGTAGATGCCTTCACATTCCGCCACT TCCTGCAGATAGCAGCAGAAGAGGACTTTCTGCGCCTGCCACTCGAGCGCCTTGTCTTCTTTCTGCAGAG CAACCGGCTGCAGAGCTGCGCCGAGATTGACCTGTTCCGCGCTGCTGTGCGCTGGCTGCAGCATGATCCT GCCCGCCGCGCCCGTGCCAGCCTTGTGCTACGCCACGTGCGCTTCCCGCTCATGCAACCTGCTGAACTGG TGGACAGCGTGCAGACACTCGACGTCATGCTCGACGATGCCCTGTGTCGCCAGTACCTGCTGGAGGCCTT CAGCTACCACGTGCTGCCCTGCCGCCAGCATGACATGCAGTCGCCTCGCACTGCTGTGCGCTCTGATGTA GCGTCCCTGGTGGCCTTCGGGGGCACACCTTATACCGACAGCGACCGAGCCGTCAGCAGCAAGGTGTTCC AGTTACCTGAGCCAGGAGCACGCCACTTCCGCGAGCTCACGGAGATGGAACTGGGCTGTAGCCACGCGGG CGTGGCTGTGCTGGATAACTTCGTGTACGTGGCAGGCGGCCAGCATCTGCAGCACCGCAGTGGCGAAGGC GCTGTGGACGCCTGCTACCGCTATGATCCACAGCGCAATCGCTGGCTGCGGCTGCGAGCACTGCGTGAGA GCCGTGTGCAGTTCCAGCTCACTGCACTGGGAGGGCTGTTGTATGCCACCGGTGGCCGAAATCGCGCGGG CAGCCTGGCGTCTGTGGAGCGCTACTGTCCACGCCGTGACAGCTGGGATTTTGCCTGCCCACTCAAGCGC CGTACCTGGGGCCATGCGGGTGCAGCAGCTGCTGGTCGCCTCTACATCTCAGGTGGTTATGGCGTCTCAG CGGAGGACAAGAAGGCGCTGCAGTGCTATGATCCAGCAGCCGACCGCTGGGAGCCCAGGGCGCCCATGCG TGAGCCCCGCGTGCTGCACGCAATGTTGGGCGCTGCTGGCCGCATCTATGCCCTAGGTGGCCGCATGGAC CACGTGGACCGCTGCTTCGATGTGCTGGCTGTGGAGTACTACGTGCCGGATGCCGACCAGTGGACCAGTG TGACACCCATGCGAGCTGGTCAGTCGGAAGCTGGCTGCTGTCTACTAGAGAGGAAGATCTACATCGTGGG TGGCTACAACTGGAGGCTGAACAATGTCACAGGCATCGTTCAGGTGTACAACACTGAGACGGATGAGTGG GAGAGAGACCTGCACTTCCCCGAGTCCTTTGCAGGCATTGCCTGTGCTGCTGTCCTGCTGCCCCGCTCAG GCCATGGGAGGTAGCTTGGGATCCTGGCCTGGCTGTGTTCCCCACAAGTGTCAGACCCCCAGCCCAGGGG TCCCTACCTCCCAGCCCCAAGTCAACAGAGAGAGAGATCTGGTCCCCAAGGTTTAGGTGCCATGGCAGCC ATTGCCCAATGGGTGTCTCTGCACTGTCCTGTGTGGTTAGGTCTTGATATCTCTCATCCCTAGCCAGACA GATGAAAGCTTGTGGACAGAAAACACCAAGTCCAGGGAGGAGCAACTCTCCTTGTCTCCTGACTAAACTC TTCTGTTTAAAATCCTAGCCTGGGTTTCTGCCGTGGGATCCACAAGGCAGAGAGACTCTTCAGAATACCC TCTGAACTCCACGTGGGCATCATGGAACACACATGCACGCACACAATCAAATGAGTAAGATTGTAAATCA GCTCTCTGGTGCCTGGACAGCATTCAGGATGCAGGAGTCAGAGTGTGTCCCAGAGGCCCCCTGACAGGTG ACAGATGCAGCCAAGTCTTCACCACCAACCTCCCTGGCCCGCTTGACTTGTGGATTCTGGAGCATCCCAA AGGCCCCCTGTCCCGTTAACCCAGCATTCTTTGCCTGTGCCACCCCCTTTTATAAGAAGACAGAGCTAAT AAGTGAGGCATGGTGTGGCCTTTTCCAATACCCTGGAGGCTAAAGAGGGAGGAGAATCATTGCAAGTTCT AGGCCAGCCAGGACCACAGCCCTGAAATGAGGAAACCCACAAAAAACAAAAGCAGAGAAACCACAAAAGC CACCTCAGGTGAGGAGGCCTGTGTCAGCTGGCCCGGGAGGAGAGCCAGCTGGGGGCCCATCTAACATGTT GGCTATAGAAAGCCATGCTCCCAGGTCTGGCCCACCCTCGGGTGGGGTGGTGGGAGGTGCTGTGGCCGTG AGCCCCAGCAGCCTCATTCGATGGCAGCCCTGCTTTGTTTTGCTGTTTAAATCCTGAACTCTCATCTTCA TATTTTGTTTAATTTTTTTCTTGTTTAAGGAAGCCAGCCCCACTGTCTTCCAAATGAGTATGTTCTCTGG TGATCTAAGGTGTCCCTTGCTGCTCAGAGTCATGGATGTCATCGTTGTACGGCCGCTCAGCACATGAGTA ATTAATCCCAGTCCTTTGCTGCAGCCTCTGCTGATCAGTCCTTTGATTTAATAAAGGCATTATGGAGGAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3594528 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3594528 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11088 same embryo
	EMAGE:11084 same embryo
	EMAGE:11087 same embryo
	EMAGE:11089 same embryo
	EMAGE:11086 same embryo
	EurExpress:euxassay_006489 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source