EMAGE mouse gene expression spatial database

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EMAGE:11114

Mab21l1 mab-21-like 1 (C. elegans) ( MGI:1333773)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001131. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001131_01	euxassay_001131_02	euxassay_001131_03	euxassay_001131_04

euxassay_001131_05	euxassay_001131_06	euxassay_001131_07	euxassay_001131_08	euxassay_001131_09

euxassay_001131_10	euxassay_001131_11	euxassay_001131_12	euxassay_001131_13	euxassay_001131_14

euxassay_001131_15	euxassay_001131_16	euxassay_001131_17	euxassay_001131_18	euxassay_001131_19

euxassay_001131_20	euxassay_001131_21	euxassay_001131_22	euxassay_001131_23	euxassay_001131_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11114_wholemount_strong.wlz

11114_wholemount_moderate.wlz

11114_wholemount_weak.wlz

11114_wholemount_possible.wlz

11114_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11114_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pituitary gland

weak

regional

weak expression: see section 11 12 13 14 15 16 17

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 20

vagus x ganglion

weak

regional

weak expression: see section 09 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 weak expression: see section 08 09 18 19 20 21

cochlea

moderate

regional

moderate expression: see section 03 04 05 22 23 24

nasal capsule

weak

regional

weak expression: see section 10 11 12 13 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4338
Entity Detected:	Mab21l1, mab-21-like 1 (C. elegans) ( MGI:1333773)
Sequence:	sense strand is shown >T4338 GCACACTCTGCACACTTTTCCTCTACCTGATTAACAGTCCTGCACACACCATGATTACGGGAAAACGTAA ATAAATACGGAAAGGGGTGATTATTTTGACTACTGGAAGAGCTTTGCTGGGTCTCAGCATAACTTTTGTT TTTATTACCGAGAAGGTGCTCTCTCCACGCGTCTCTCTCTGACACGGATTCTTTAGCGGAGGGGAGAAAA GGGGAGAGCTCAGCCTTTCACTTTAAGACCCGCTAGTCTCGAGCGGGGAAAAAAAGGCCAGCAGGGCAGG GCGGCCCCCTGCTGCAGCTAGTCCGAGAGAGGCTCGGTGCCTTTTGAGCGTGGTCCCCTTTCTCCACCGA AGTTGGCTCCAGCGCTAGCAGCCGCATTGGATCCCACAGCCTATTGCGAGACTCCGGTGTACAATCCGGA TCTCTGCCCCAACATGATCGCGGCCCAGGCCAAGCTGGTCTACCACCTGAATAAGTACTACAACGAGAAA TGCCAGGCCAGGAAAGCTGCCATCGCCAAAACCATCCGGGAAGTCTGCAAAGTGGTCTCCGACGTCCTGA AGGAGGTTGAAGTGCAGGAACCGCGTTTCATCAGCTCCCTCAATGAGATGGACAACCGCTACGAGGGCCT GGAGGTTATCTCTCCCACCGAGTTCGAGGTGGTACTCTATCTGAACCAGATGGGGGTGTTTAACTTCGTG GACGACGGCTCCCTGCCTGGCTGTGCGGTGCTGAAACTGAGCGATGGGCGCAAGAGGAGCATGTCCCTCT GGGTGGAATTCATCACCGCCTCTGGCTACCTCTCGGCACGCAAAATCCGTTCCAGGTTTCAGACACTGGT GGCTCAGGCGGTGGACAAGTGTAGCTACAGAGATGTAGTAAAGATGGTGGCTGACACGAGCGAAGTGAAA CTGAGAATCCGAGATAGGTATGTGGTGCAGATCACCCCGGCCTTTAAATGCACCGGGATTTGGCCGCGGA GTGCTGCCCACTGGCCGCTTCCCCACATCCCCTGGCCAGGACCCAACCGGGTGGCAGAGGTTAAGGCTGA AGGGTTCAATTTGTTGTCCAAGGAGTGCCACTCCCTGGCCGGCAAGCAGAGTTCGGCTGAGAGCGATGCT TGGGTGCTGCAGTTTGCAGAGGCCGAGAACAGACTGCAGATGGGGGGCTGCAGGAAGAAATGCCTCTCTA TCCTGAAAACCCTGCGTGATCGCCACCTGGAGTTGCCAGGCCAGCCCCTGAACAACTACCACATGAAGAC TCTGGTGTCCTACGAGTGCGAGAAGCATCCTCGAGAGTCGGACTGGGACGAATCTTGCCTGGGCGATCGG CTGAACGGAATTCTTCTGCAGCTCATCTCATGCCTGCAGTGCCGGCGGTGTCCCCATTACTTCTTACCGA ACTTAGATCTGTTTCAAGGCAAACCCCACTCGGCCTTGGAAAACGCTGCTAAACAAACGTGGCGACTGGC AAGAGAGATCCTGACCAACCCAAAAAGTTTGGAAAAACTTTAGAGGATGATCTAATCCAGGGCTGACAAG AATGAGCATTCTTCTCAAAGTCCCGACGAAATGAAAACTTCCTGGTTGATTCCTATGTACTATTTCTCGA GACAGTGCAAACAATCTCTTCCTTTAAAAGGAGTAACAACACAACATTTAACCATCATCTCACACAGGGG AAAAACCACACCACAAGCATCTTTATGGAGGCATCCCCCAGCTCCCCCGAAAAGTGCACAACACCCCAAA ACAGCTGGCTGCAATGCAAGACCATGGCCAACTCCAGCAAAGGATTATAGTAATCTTGAGACTTAGGTGC GGCTGCCTTCCAGCAAACAGGGCCCGGATGTACCATCGGAAGCAAAGTGTAATGTATATTTTGATCTGTA ACAAAAGTTGTGATCTCATGTTGTCTTTGAAAGTGTGGATATTGGTGTTTTGTGATTTGGTGATCAGAAC TGAAATTACCATTTTGGAAACTTTCAGACATTTCCCACAGAACAGAGATGCATTTAAAGGTAGATTTCCT CCTGGTACTTTACCTGTCTTTCAAGTGTCTGAACTTAAAACAAAAGTTTACATTTTGTTTCAAATATATT GCTTGTTCTAACATTCCGTAAGTATACTTGAAATGTTATTTAAATATATTCAAAAATTTGAATTCAGCTT ATATAATAACGCTTGAATATCTGAATTATATATATATTTGAAAATGCACTTGAAAATACACTGGATAATT ACTTTTGTGATTTAGAATTTAATTTCTGTTGCTGATTTTTATTTAATTAGAAGATAATAAATGAAGTAAA ATACCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4526962 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4526962 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11113 same embryo
	EMAGE:11117 same embryo
	EMAGE:11112 same embryo
	EMAGE:11116 same embryo
	EMAGE:11115 same embryo
	EurExpress:euxassay_001131 same experiment
	MGI:4826051 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source