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EMAGE:11123

Gprc5a G protein-coupled receptor, family C, group 5, member A ( MGI:1891250)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11123 EMAGE:11123 EMAGE:11123 EMAGE:11123 EMAGE:11123
"Pseudo-wholemount" of euxassay_001012. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001012_01 euxassay_001012_02 euxassay_001012_03 euxassay_001012_04
EMAGE:11123 EMAGE:11123 EMAGE:11123 EMAGE:11123 EMAGE:11123
euxassay_001012_05 euxassay_001012_06 euxassay_001012_07 euxassay_001012_08 euxassay_001012_09
EMAGE:11123 EMAGE:11123 EMAGE:11123 EMAGE:11123 EMAGE:11123
euxassay_001012_10 euxassay_001012_11 euxassay_001012_12 euxassay_001012_13 euxassay_001012_14
EMAGE:11123 EMAGE:11123 EMAGE:11123 EMAGE:11123 EMAGE:11123
euxassay_001012_15 euxassay_001012_16 euxassay_001012_17 euxassay_001012_18 euxassay_001012_19
EMAGE:11123 EMAGE:11123 EMAGE:11123 EMAGE:11123 EMAGE:11123
euxassay_001012_20 euxassay_001012_21 euxassay_001012_22 euxassay_001012_23 euxassay_001012_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11123Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11123_wholemount_strong.wlz
11123_wholemount_moderate.wlz
11123_wholemount_weak.wlz
11123_wholemount_possible.wlz
11123_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11123_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
stomach
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10 11
hindgut
weak weak
regionalweak expression: see section 16
midgut
weak weak
regionalweak expression: see section 10 11 12 13 14 15 16 17
kidney calyx
weak weak
regionalweak expression: see section 09 10 11 12 19 20 21 22 23
laryngeal cartilage
moderate moderate
regionalmoderate expression: see section 12
lung
moderate moderate
spottedmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
main bronchus
moderate moderate
regionalmoderate expression: see section 14 15
trachea cartilaginous ring
moderate moderate
regionalmoderate expression: see section 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1087
Entity Detected:Gprc5a, G protein-coupled receptor, family C, group 5, member A ( MGI:1891250)
Sequence:sense strand is shown

>T1087
GAGTCGCAAGTTCTCGCTTCACCCCGAGCGCCGCGGCGCCCAGGACCAACCAGAATGACTACTCCTAGAG
CTGCCCCTAGCGGTTGCCGCTCAGACCTGGATTCCAGGTACCACAGACTTTGTGACCTGGCGGAAGGCTG
GGGCATCGCGTTGGAAACACTGGCTGCCGTCGGGGCTGTGGCTACCGTGGCTTGCATGTTTGCACTCGTT
TTCCTCATCTGCAAAGTGCAGGACTCCAACAAAAGAAAGATGCTCCCCGCCCAGTTCCTCTTCCTCCTGG
GAGTGCTAGGGGTCTTTGGCCTCACCTTCGCCTTCATCATCAAGCTGGATGGGGCCACAGGACCCACGCG
CTTCTTCCTCTTCGGAGTCCTCTTCGCCATCTGCTTCTCTTGCCTCCTGGCCCACGCTTTCAACTTGATC
AAGCTGGTCCGAGGGAGGAAGCCCCTGTCGTGGCTTGTGATCCTCAGCCTGGCGGTGGGCTTCAG
Notes:The probe template was PCR amplified from IMAGE:2123111 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2123111 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11126 same embryo
 EMAGE:11124 same embryo
 EMAGE:11125 same embryo
 EMAGE:11122 same embryo
 EMAGE:11127 same embryo
 EurExpress:euxassay_001012 same experiment
 MGI:4825200 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS