EMAGE mouse gene expression spatial database

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EMAGE:11154

Cbwd1 COBW domain containing 1 ( MGI:2385089)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003579. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003579_01	euxassay_003579_02	euxassay_003579_03	euxassay_003579_04

euxassay_003579_05	euxassay_003579_06	euxassay_003579_07	euxassay_003579_08	euxassay_003579_09

euxassay_003579_10	euxassay_003579_11	euxassay_003579_12	euxassay_003579_13	euxassay_003579_14

euxassay_003579_15	euxassay_003579_16	euxassay_003579_17	euxassay_003579_18	euxassay_003579_19

euxassay_003579_20	euxassay_003579_21	euxassay_003579_22	euxassay_003579_23	euxassay_003579_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11154_wholemount_strong.wlz

11154_wholemount_moderate.wlz

11154_wholemount_weak.wlz

11154_wholemount_possible.wlz

11154_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11154_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 05 06 07 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6712
Entity Detected:	Cbwd1, COBW domain containing 1 ( MGI:2385089)
Sequence:	sense strand is shown >T6712 TGGAATCACTGGTGTCCTGCTGGCGGCATGTTACCTGCTGTGAAAACTGTGGAGGCGGAAGAAGAGTACG CGGAGGATTGTCCTGAATTGGTCCCCATTGAGACCAAGAACCAAGAAGAGGAAAATCTTGACTTCATCAC TAAGATTCCAGTCACAATTGTCACCGGGTACTTAGGTGCTGGAAAGACAACACTTCTGAACTACATTTTG ACAGAACAACATAATAGAAAAATAGCAGTGATTTTAAATGAATTTGGAGAAGGGAGTGCAGTAGAGAAAT CCTTAGCTGTCAGTCAAGGTGGAGAACTCTATGAAGAATGGCTGGAACTTAGAAATGGTTGCCTCTGCTG TTCAGTCAAGGACAGTGGCCTTAGAGCTATAGAGAATCTGATGCAAAAGAAGGGCAAGTTTGACTACATA CTCTTAGAGACCACTGGGCTAGCAGATCCTGGTGCAGTGGCTTCTATGTTTTGGGTTGATGCTGAGTTAG GGAGTGATATTTATCTTGATGGTATCATAACTGTTGTAGATTCAAAATATGGATTAAAACATTTAACTGA AGAAAAACCTGATGGCCTTGTCAATGAAGCTACTAGGCAAGTTGCATTGGCAGACATGATTCTCATCAAT AAAACAGACTTGGTTTCAGAAGAGGAGCTAAACAACTTAAGAACAACTATCAGATCAATAAATGGACTGG GAAAAGTTTTAGAAACTCAGAGATCAAGAGTTCATCTCTCAAATATATTAGACCTTCATGCCTATGATAT TCTCTCTGGAATAAGTTTACAGAAAAAGCTCCAGCATGTGTCAACAGCACCCCACCTTGATCAGAGTATT GTTACAGTCACATTTGAAGTACCAGGAAGTGCAAAGGAAGAGTGTCTAAATGTATTTATCCAGAATCTCC TGTGGGAAAAGAACGTGAAAAACAAGGACGGCCACTGCATGGAGGTCATTCGGCTAAAGGGACTGGTGTC TATCAAAGACAAACCACAGCAAATGATTGTGCAAGGCATCCATGAGCTGTACGACCTGGAAGAAAGCCTA GTGAACTGGAAGGATGATGCGGAGCGAGCGTGTCAGCTGGTCTTCATTGGTAGAAATTTAGATAAGGATG TTCTTCAACAACTCTTCCTCACTGCTGTGGCAGAGGCAGAAGAGCAAAGGACAGCACCAGGCAGAGATGG AGTTTGTCCATCGCACTAGAAGCTGTTTCTTACCGGAAGAACTGACGAGAAAAACAAAGATGTATTTAAA TCCTGGATCCACTACCGCTGTTGTGATTTGGGAGTATACGTATAATAGTATTTATTTTATAAAAACACAT AACATTGTAGTAAATTATTATAAAAAATATCTGATATTCATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3661842 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3661842 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11157 same embryo
	EMAGE:11153 same embryo
	EMAGE:11152 same embryo
	EMAGE:11155 same embryo
	EMAGE:11156 same embryo
	EurExpress:euxassay_003579 same experiment
	MGI:4823657 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source