EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11155

Tmem64 transmembrane protein 64 ( MGI:2140359)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003576. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003576_01	euxassay_003576_02	euxassay_003576_03	euxassay_003576_04

euxassay_003576_05	euxassay_003576_06	euxassay_003576_07	euxassay_003576_08	euxassay_003576_09

euxassay_003576_10	euxassay_003576_11	euxassay_003576_12	euxassay_003576_13	euxassay_003576_14

euxassay_003576_15	euxassay_003576_16	euxassay_003576_17	euxassay_003576_18	euxassay_003576_19

euxassay_003576_20	euxassay_003576_21	euxassay_003576_22	euxassay_003576_23	euxassay_003576_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11155_wholemount_strong.wlz

11155_wholemount_moderate.wlz

11155_wholemount_weak.wlz

11155_wholemount_possible.wlz

11155_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11155_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 22 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 15 16 weak expression: see section 14

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 19 20 21 22

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 09 10 11 15 16

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 13 14 15

testis

strong

regional

strong expression: see section 06 07 08 09 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6706
Entity Detected:	Tmem64, transmembrane protein 64 ( MGI:2140359)
Sequence:	sense strand is shown >T6706 CCACGCGTCCGCGGCCGTTCCCTCTCCGCGAGAGGACGCAGGGAGCCGAGGCCTGGCGTGCGGGGTGCCA TGGAAGGCCGCAGGCGGGGGGACGCGGCGGGAGTGGAGACACTGGCCGGCTGAGGCCGAGGCATGCGGAG CCCCGGTGGGAGCCTGCCCCACACGCTGCCCCGGGCCTTGCAGCACGCCGGTCGGACGGGCGTCGTGGAG CAGCCGGGCCGCTGGGCACCGGAGCGGACAGCGGGAGGGGACCGCTCGGAGGACCGCCTTCCCCGCGGGG GCGGGGCCAGCGCGGCGGCGGCTGCTGCTGCGGCGGCGGCCTCGGGCGCCCTGCTCGGCGCCTATCTGGA GCGCCACGGTCTGCCCGCGGCCTCGGACTTGCCGGCGCCGGCCGGGGCGTTGGCAGGCGGCCCCGGGAGC GGCGGCGGCGTGGTGGTCGGGGTGGCCGAGGTGAGAAACTGGCGCTGCTGCTGCCTCGGCAGCACCTGTT GGTGCCGGAGCCTCGTGCTGGTGTGCGTGCTGGCCGCCCTGTGCTTCGCTTCCCTGGCCCTGGTCCGCCG CTACCTGCAGCACCTCCTGCTCTGGGTGGAGAGCCTCGACTCGCTGCTCGGTGTCCTGCTCTTCGTCGTG GGCTTCATCGTGGTCTCCTTCCCCTGCGGTTGGGGCTACATCGTGCTTAATGTGGCGGCCGGCTACCTGT ACGGCTTCGTGCTAGGCATGGGGCTCATGGTGGTGGGCGTCCTCATTGGCACCTTTATCGCTCATGTGGT CTGCAAGCGGCTACTCACCGCCTGGGTGGCTGCCAGGATCCAGAACAGCGACAAGCTGAGCGCCGTTATC CGCGTCGTGGAGGGAGGAAGCGGCCTGAAGGTGGTGGCGCTGGCCCGGCTGACTCCCATACCTTTTGGGC TTCAGAATGCAGTGTTTTCGATTACTGACGTCCCCTTGCCCAGCTACCTGATGGCGTCTTCAGCTGGGCT GCTACCCACTCAGCTGCTGAATTCTTACTTGGGGACCACACTACGGACTATGGAAGATGTCATCGCAGAA CAAAGTCTTAGTGGCTATTTTGTCTTTTGTTTACAGATTGTTATAAGCATTGGCCTCATGTTTTATGTAG TCCATCGCGCTCAAGTGGAATTGAATGCAGCTATTGTAGCTTGTGAGATGGAACTGAAAACCTCTCTGGT TAAAGGCAATCAATTGGATCCCAGTGGCTCTTCCTTCTACAACAAGAGGACCCTCACGTTTTCTGGAGGT GGAATCAATATTGTATGATTCTTACAAACTGTGATTGTTGAGAGCCTAGCGTGCTGTCCAGGCCTAGCAG TCACTTACCTGATGGCAGAGACAAGTGCTGTTGTATTATGGCACAAACAAAACTGACTAGTTTTTAAATT GCACAATTTTCTAAAAGCAAGAATCATTTTCTGGGTCTGTCAGTGTAAATGTAGATGCAGCTCTGGCTGC CCCCCCCCTTGTTATACCTGTAATGGCCTATAGGTGTGCACTTTAGTGTTTTTGAATTATTTTATTGCTG GGACTTATGAACAGAAAAATAACCCAAATATTTTCTTTCTGTTTAGTATCTTTATTTATAAAGCCCATAA ATAGTTGTGTGCATCTGTCTTTAAGGATGAGTGAAAGTATAGAGTTCGCAATGGCTCTTGTTGTATGCTC TTTGCTTAGGCATTCTCTCCAGAAGGGTGAACTGTTTTGTTTAATGTTCACTTGCTTGTTTCAAGCAGAA AAGCTCTGCTTTGTTTTCCTTGCTTGTAAGGAAATAGCTGTATTTCGGTTGACTGTATGTTATCCGGCAT TATGTCACTGTGGTCATTTACACCTCTCGGTACAAGTGTAAACCCTACATTGTGGGACTGAAGTGCTCTT AAGACCCCTTTTATTTTCACTGTGTAAGATGCAAAGTGAAAGGGACATTATTTAGCAGGTGGCTCACAAG TCGAGCCCGCATTCTTATTCGATTATATTGGTTTCTCCCATTAGACCTCATACAAGGGCTGTCCGATAGC AACTCTTACCAAACCATTTCCCCCTTTTATATTAATAATTAGGCTTTGAAATAAACTTATTGTTCCTATA AAATGGTGGGCTTACCATCTCTGACGTCATTCAGGTGGCCTTGTTTAGTCAAAACGCCTTGTTAAAAAGA AACGAGCTTTAAAATCATGTTTATAGTTTCAGTTAAAAAATATATATATGTCCCCATAGCTTTCAGCTTT AAAACTATAAACATTAACAAAAATCCCACATTCGTGCTGTCTGCTGCGTTATTGCTGTTTTGTTTGGGGC TTTGGTGTTCCTTTTCCCTGACAAGACTCAGGAATTCTGAAATGTGAAATTGTCTCAATCCTTTATCTTG TAGCATGTGTGTTTGCTAACAGCACTTAGGACTTAGCATACAATAATCCAACATGATTCGTATGGCGTGC ACTCATTTATCAGCTTTATTTTTAAAATAGTTTAATTGCAAACTTAATGATGAGTTAATTTTGTTGCAAA TAGATTGTAGTTGATACGGTAACGTTTTCTGTTAAGGACTTCACTTTTCTATGACTGTTCCAACCTATTA GAGCTCTCTCCGCTGCCTTTTGCTGGCAGTAAAACCTTTGCCCTGGGTACTGCTGACATCCAGAGAAATC CCGCGCAAGCAGATTTTTCTAGTCTTAACCCCAGAGAAGAGTTTGATTGTGTTCACACACGAGGTTTTTG CCCAGTTCAGGTGGCTTTCCTTCCCCTGCTGATGCGTAAATCCCTCTGCGTCTGAGTAGCAGTAGAAGAC CAGAGTAACTGGGACGAAAGGCACCTGGTCTACAGTGCATGCCTGGCCTCCAGACCTTTGTTATCGGTAG AAAAACAACTATACATACCGAGTCCTTACTGTCTGTTTAGTTAAGTTTCAGTTAGTTTAACCATCCCAAT AAAAGAAATATTTGATTTATTCTTTTTAGTACAACTTGGCAACATTTTTCTTGTGTTTCTCATTGGCTAT TTTTTGTTAAAGGAATCTTTATTTGAGATCATTATTTTCATGCTTGCTTTACAATTCATGTATTTTGAAG TGCATTATATTTACTTAGCATTTGTAACTTGAACGACTTCACCAAATGAATACCTTTTGGTAATTTGTAA TAACTTCTTCCAAATGTTAACACTTTCCCTGGTAATTGTATTACATATGTAAAGACAAAAGAAATACTTT TCTGTATTCTGCCAATCATAATTTTATATAATAAATCATCAACTTTTATAATAGTATGGATTGACAATTT CTAAAGTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3661112 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3661112 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11157 same embryo
	EMAGE:11153 same embryo
	EMAGE:11152 same embryo
	EMAGE:11154 same embryo
	EMAGE:11156 same embryo
	EurExpress:euxassay_003576 same experiment
	MGI:4828813 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source