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EMAGE:11158

Iqcc IQ motif containing C ( MGI:2446212)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11158 EMAGE:11158 EMAGE:11158 EMAGE:11158 EMAGE:11158
"Pseudo-wholemount" of euxassay_003583. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003583_01 euxassay_003583_02 euxassay_003583_03 euxassay_003583_04
EMAGE:11158 EMAGE:11158 EMAGE:11158 EMAGE:11158 EMAGE:11158
euxassay_003583_05 euxassay_003583_06 euxassay_003583_07 euxassay_003583_08 euxassay_003583_09
EMAGE:11158 EMAGE:11158 EMAGE:11158 EMAGE:11158 EMAGE:11158
euxassay_003583_10 euxassay_003583_11 euxassay_003583_12 euxassay_003583_13 euxassay_003583_14
EMAGE:11158 EMAGE:11158 EMAGE:11158 EMAGE:11158 EMAGE:11158
euxassay_003583_15 euxassay_003583_16 euxassay_003583_17 euxassay_003583_18 euxassay_003583_19
EMAGE:11158 EMAGE:11158 EMAGE:11158 EMAGE:11158
euxassay_003583_20 euxassay_003583_21 euxassay_003583_22 euxassay_003583_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11158Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11158_wholemount_strong.wlz
11158_wholemount_moderate.wlz
11158_wholemount_weak.wlz
11158_wholemount_possible.wlz
11158_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11158_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6722
Entity Detected:Iqcc, IQ motif containing C ( MGI:2446212)
Sequence:sense strand is shown

>T6722
GCGGTTGGCGAGGAGATGGATCCCGACCTTTTCCTTCGAAAGGTGTCTACATTGCAGGCCGGCTTCCGAG
GCTTCTTGGTCCGACGTCAATTCCAGAGCCTTCGAGCCGAGTATGAGGCAATTGTACAGGAGATTGAGGG
TGACCTCAGCACTCTTCAGTGGACTGGGGGCTGGATCCCCAAGCCCGTATTTCTCCCAGAGGCAAAATCT
CATCAGTCCTGGAAAGCGGAGAAAATATCAAACCCAGAGCAGAAACTATGGAGCCATTCCCCACATAAAG
ACTCTGAAAAAGAGCTCATTTGGGAGGAGATGGTGCTGAAGAAGACAGAAAAGAGCCCAGCAAATCCAGG
AAGTCTTTGCAGAGGTGACAGCGCCTGGCCGCAGGCTGAGCAGGGCAGGAAAGCCAGCCAGGGAAACAGC
CAAGATACCTCTGTCTCCAAGATGGAAAATGCAGACCTAGGATTGTCCCAGAGCCAACAGGAGCTCCAGG
AGCAGCGCAACCACTTAGCCATGGAGCTGCTGTGGCTACAGCAGGCCATCAACAGTCGAAAGGAGTACCT
AATTCTCAAGCAAACACTGAGATCCCCAGAAGCAAGCCAGACCAGAGACAAGCACAGGGGACAGGCCTAT
GAGAAGACCTCGTTGCACTCAAGCTGCGTTCTGGACAACCAATCCTATAGAGACAGGATCATAGGAGAGT
CCCATCATGCAGAGGACTCCAGCCACAAGGGCAGATTGAAGCCCCAGAAACACCCAGACAGTGTGACCAG
TGCAGGTAAAACCACTGCTGGGTCTAAAGGCAGGGAGCTCTGCTATAGGAACTCTGCATCACAGCTGCCT
GCAGCATTAGAGAGCCAGGCTGGAGGGGACAGGGTCACCAAAGGGCCAGACCACAGAGGACAGCCCTTTA
AGGAAACCTCCCTACAGCAGTTGAAAGTCCTAGAGGATCAGATCCCTGGGGACCTCAAGTTTAGGAGTCC
TTGTTCTAGAAAGGCTGAAACACAGCTGCCCACTCTCTCTGAAAATCTAAATATTGAGGACAGATATTCC
AGAAAGCCAAGGTACAAGGAGGCTGACAGCCGAAGCGCTGGGCCCTGTGATTTGAGTATCTCAGAAGGCC
ACATGATTTGGGATGAGACCTTGGCAGGGCAGGAACAAGGTAGCCTGGATCTCATTAGGACTAAACCACC
CAAGAGCCAACCCCCGAGTGCTGGCTCTTCAGGCCATGGAAATACCAGTGAGCTTAGTCCAGAAGGATGG
AAAAACTGTGGGATTCTGCAGTAGCGATGAAGACCACTGGAGAACTTGTCTTCCTCAAGACAGGACTAGG
GGAAGGGCAGATACCGGAAAGCAGGAGCATCAGGAAAGACCTGGGGATCCAGGAGCGGAAGGGTTCCCAA
AGGAGATGCTGTGAAAAATGACAAGGGAAGCAGGCCCTTGTCCTGCCTCCCTGGTACCACCTGTCTCTCT
GCCCTTGCCCCTGGCCATCAGTAGATCTCAGGCTAAGAGAAAGAAGGACATGCCTTTTTACCTTCTGCCT
GGGACTTAAACCAAATTTGACCTCCTCTTGGGGTAAGTAGGGTGGGAGGATCCTGGAGAATAAAAGTTTT
CCTTGGATTCTTGGCTGCCATGCAGGAGGAGCTGTGTGGGCAAGCCCTGGAGACAGCTGGCTCACCGTCT
TGCTTGCTCGGCTGCCATGGGTGTCCTCGGGTGAGGCATGGCCCCAGAGACTTCGTCCCAGAATTGCTTC
TGGCTGCTGATGTGCTTCCTCATGTCTGTCATTGCCACACTCCTCACACACGTGACTTGGTGTGAGTGGA
CACCGGGAGACCCTCACTGCCTATAGCTGCTGTAATCACTTCCCAGTGACAGCCACCCCACTGGGCAAAT
GTCCTGCAGATCAACATGAAGATGAACTTTCACAAATCAATGCTGTTTACATGAATTAATGACTATAGAA
ATGATTTGAATGATTTTAGGAAGAAAGTTTAAGGAGGTGGTTTTAGTTGTTTTGCCAGGAAGACGTAATA
AAGTTAGAGTCAAGAATAGCGAATAGCGAGTACCCCCTCCTCATAGAATATTAAATAAAGGTTGCATAAT
AAAACATTTCACGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGTGGTTTTCTGAGTTCGAGGCCAG
CCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTATACAGAGAAACCCTGTCTTGTTTCTCTGTGTA
GCCCTGGCTATCCTGGGGGTGGTGGGAAGAAGAGAAATGGGCTTTGGACAGATTTGTGATCAAGGAAAAC
CATTCATCCTAGGCCAGGTCCAAGGATGAAGCCACAGGTGTGCACTATGATAAAGCAAGGTCATGGGAAA
CTGTTGCTTTGAACTTACAATGAGCTTGTAGAACGAAGCACTGCTTTGAACTGACTATCGACATCTTCTG
AAACTCCTTTTTGTGTAAACTTTTTACCTGTGAGGTAATTTTATAAAGAACTTTTGTCTAAGAAGGTATA
AAAAAGTTGAGAAAAAAAGTGTGGCTGCTGGGGCTCTGCTCCACCCAACAAATATAAATATATATATTAT
GTATAAACAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3665627 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3665627 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11163 same embryo
 EMAGE:11161 same embryo
 EMAGE:11160 same embryo
 EMAGE:11162 same embryo
 EMAGE:11159 same embryo
 EurExpress:euxassay_003583 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS