EMAGE mouse gene expression spatial database

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EMAGE:11162

Lrrc45 leucine rich repeat containing 45 ( MGI:2387183)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003587. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003587_01	euxassay_003587_02	euxassay_003587_03	euxassay_003587_04

euxassay_003587_05	euxassay_003587_06	euxassay_003587_07	euxassay_003587_08	euxassay_003587_09

euxassay_003587_10	euxassay_003587_11	euxassay_003587_12	euxassay_003587_13	euxassay_003587_14

euxassay_003587_15	euxassay_003587_16	euxassay_003587_17	euxassay_003587_18	euxassay_003587_19

euxassay_003587_20	euxassay_003587_21	euxassay_003587_22	euxassay_003587_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11162_wholemount_strong.wlz

11162_wholemount_moderate.wlz

11162_wholemount_weak.wlz

11162_wholemount_possible.wlz

11162_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11162_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6718
Entity Detected:	Lrrc45, leucine rich repeat containing 45 ( MGI:2387183)
Sequence:	sense strand is shown >T6718 CGCGAGGAACCGCGTCCTGCCACACCAGCGACCCGAGGCTACGTCGCGAGGGCGGGCTGGGGGCCACAGG GCCAGACTAGGGGGCATGCTTGCGAGGCTCCGTCAGCATTCGGGGACATGGAGGAGTTCCGGCGCTCCTA CAACCGCCTCTGCGAGGAGAGTGGGGCTGAGCCCCAGGAGGCTGTTCTGCAGCAACTGCACCAGCTTCCG AAGGGCGGACTGGACCTGACTACTCAGAGCCTGACGGTGGAGACCTGCAGGGCCCTCGGCAAGCTGCTGC ATAAGGAGACGCTGTTGAAGGAGCTTGTCCTCAGCGACTGCATGCTGAGTGAAGAAGGGTCCACACTGCT GTTCCAAGGGCTGTGTGCCAACACCAGTGTGCAGCATCTGGATCTAAAGGGCAACAACCTCCGAGCTACA GGAGCTGAGGCTCTGGGAAAGCTCCTTCGACAGAACAAGTCTATTCAGAGCCTCACGCTGGAGTGGAACA ACCTTGGCACGTGGGAAGATGCCTTTGCCACCTTCTGTGGGGGTCTGGCAGCCAATAGTGCCCTGCGGCA GCTGGACCTCCGAAACAACCAGATCAGTCACAAGGGAGCAGAGGAACTGGCCCTGGCCCTGAAGGGCAAC ACCACCCTCCAACAGCTGGACCTGCGCTGGAATAACATTGGCCTCCTGGGGGGCCGGGCTCTGGTGAACT GTCTCCCCAGCAACAGAACCCTGTGGAAGCTGGACCTGGCTGGGAACAACATCCCTGGTGACATCCTGAG AGCCGTGGAGCAAGCCATGGACCACAACCAGGACCGACTCACTGCCTTTCGGGAGAACCAAGCACGCACC AAGATCCTTAGCAAGGAAGTTCAACACCTCCAGGAGGAGAAGTCCAAGCAGTTTCTGGATTTGATGGAGA CAATAGACAAGCAACGAGAAGAGATGGCCAGAGATAGCCGGGCATCAGCAGTACGGGTTGGGCAGCTTCA GGAAGCCCTCAACGAGAGGCAGTCCATCATCAATGCTCTCAAAGCCAAACTGCAGATGACAGAGGCTGCT CTGGCTCTGTCAGAACAGAAGGTCCGGGACCTGGGGGAACTGCTGGTCGCCGGAGACCAGGAACGGCAGA GCCTGTCCCAAAGACATGAGAAGGAACGCAAGCTGGAGCGGCAGGAAGCTGCAGATCGGGAGTCCAAGTT ACTCAGGGACCTGTCTGCTGCCAACGAAAAGAACCTGCTGCTTAGAAGCCAGGTGGATGAGTTGGAGCGG AAGGCGAGGTCACAGCAGGAGCAGCTGTTTCTGACCAAGCAGGAGCTGACTAACACGTCGGCCGAGTTGA AGATCCGTGCTATTCAGGCTGAGGAGCGCTTGGATGTGGAGAAGAGGAGAGCCAAACAGAACATGGAAGA CTTGGAAAAGCTACACTCCAAGGAGGTGGATCATATGACTCGTCACCTGGAGGAGAGTGAGAGGGCCATG CAGGAGAGGGTTCAGAGACTCGAGGCCCTGCGGCTGTCTCTGGAGGAGGAGCTGAGCCGCATGAAGGCCG CAGTGCTCAGCGAGCGTGGCCAAGCTGAGGAGGAGCTCATCAAAGCCAGGAACCAGGCCCGCTTGGAGGA GCAACACCGCCTTGCTCATCTGGAGGAAAAGATCCGGCTGTTGGCACAGGCAAGGGACGAGGCTCAGGGC ACCTGCGTGCAGCAGAAGCAGATGGTAGCCGAGTCCCAGGCACGGGTCAGCCAGCTGAACCTGCAGATGG AGGGGCAGCAGCGGCGTCTGGAGGAGCTGCAACAGGAGCTGATCAACAAAGACCAAGAAAAAGTGGCCGA AGTGGCCAGGGTCAGAGTAGAGCTGCAGGAACAGATGGGCCGTATGCAGGCTGACCTGGTGGCCCAGGAG GCGCTGAGAGAGAAGGTGGCAGCCCTGGAGCGTCAAATGAAAGTGATCGGGAGTGAACACCGGGAGGCGC TGTTGGACAGAGAGAGTGAGAACGCATCTCTCCGAGAGAAGCTGCGGCTCAAAGAGGCAGAAATCTCCCG TATCCGGGACGAAGAGGCCCAGAGGGCAAGCTTCCTGCAAAACGCTGTCTTGGCTTACGTGCAGGGGTCC CCTCTGAGAGCCTTGAGTCCCCCTAAATGAAGGGAAGCGGGAGAACCGAAGCGCCCAGCGAGGGTAGAAA TGCTGCATCCCATTCTCAAGGGCAGGCCCGGGGTCCAAAAAGCATCAGCTGCAGTTGCAGGGCAGGGTTC TGTCAACATCGGTGCTCAGCGGCACACCTGCCCGTCCAAGGGACTTCATCCACAGTTCACCGTTAGCATA GGCGCGATGAAGCATACAGGATCGCGCTCAGACAACCCGGTCCCAGAACCACAAACAGGACAGGACAGCG GTGGGAGGAAGCAGACTCCCCAATGGCAGGGTGGAAATACAGAACTTTTGTACTTGGTGATAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3662961 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3662961 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11163 same embryo
	EMAGE:11161 same embryo
	EMAGE:11158 same embryo
	EMAGE:11160 same embryo
	EMAGE:11159 same embryo
	EurExpress:euxassay_003587 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source