EMAGE mouse gene expression spatial database

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EMAGE:11205

Hmox2 heme oxygenase (decycling) 2 ( MGI:109373)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003408. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003408_01	euxassay_003408_02	euxassay_003408_03	euxassay_003408_04

euxassay_003408_05	euxassay_003408_06	euxassay_003408_07	euxassay_003408_08	euxassay_003408_09

euxassay_003408_10	euxassay_003408_11	euxassay_003408_12	euxassay_003408_13	euxassay_003408_14

euxassay_003408_15	euxassay_003408_16	euxassay_003408_17	euxassay_003408_18	euxassay_003408_19

euxassay_003408_20	euxassay_003408_21	euxassay_003408_22	euxassay_003408_23	euxassay_003408_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11205_wholemount_strong.wlz

11205_wholemount_moderate.wlz

11205_wholemount_weak.wlz

11205_wholemount_possible.wlz

11205_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11205_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

spotted

moderate expression: see section 13 14 15 16

submandibular gland primordium

moderate

spotted

moderate expression: see section 08 09 17 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 09 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 05 moderate expression: see section 06 07 08 09 18 19 20 21 22 23

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 17 18 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 15 16 17 18 19 20

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13

lower jaw incisor

moderate

spotted

moderate expression: see section 11 12 15 16 17

lower jaw molar

moderate

spotted

moderate expression: see section 06 07 08 09 18 19 20 21 22

upper jaw incisor

moderate

spotted

moderate expression: see section 12 15 16

upper jaw molar

moderate

spotted

moderate expression: see section 06 07 08 09 18 19 20 21 22

lung

moderate

spotted

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

orbito-sphenoid

moderate

spotted

moderate expression: see section 02 03 04 05 06 22 23 24

not examined

spotted

not examined expression: see section 08 09 10 11 12 13 14 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5955
Entity Detected:	Hmox2, heme oxygenase (decycling) 2 ( MGI:109373)
Sequence:	sense strand is shown >T5955 GTCCTGGCGCTCGCCCTCTCGGCTGCGAAGGTACAGAGAGAGTCGCAGACGCGGAGACTGACTGACCTGC GGAGCCACTGACGGACCAGAGTGAGGGCAGCACAAACTACTCAGCCACAATGTCTTCAGAGGTGGAGACC TCGGAGGGGGTAGATGAGTCAGAGAAGAACTCTATGGCACCAGAAAAGGAAAACCATACCAAAATGGCAG ACCTTTCTGAGCTCCTGAAGGAAGGGACCAAGGAAGCACATGACCGAGCAGAAAATACCCAGTTTGTCAA AGACTTCTTGAAAGGAAACATTAAGAAGGAGCTATTTAAGCTGGCCACCACTGCACTTTACTTCACATAC TCAGCCCTTGAGGAGGAAATGGACCGCAACAAGGACCACCCAGCCTTCGCCCCCTTATATTTCCCCACGG AGCTACACCGGAAGGCAGCACTGATCAAGGACATGAAGTATTTCTTTGGTGAAAACTGGGAGGAGCAGGT GAAGTGCTCTGAGGCTGCCCAGAAGTATGTGGATCGGATTCACTATGTAGGGCAAAATGAGCCAGAGCTG CTGGTGGCCCATGCTTATACTCGTTACATGGGGGACCTTTCAGGAGGCCAGGTACTGAAGAAGGTTGCCC AGAGGGCACTAAAACTTCCCAGCACTGGGGAAGGGACCCAATTCTACCTGTTTGAGCATGTGGACAATGC CCAGCAATTCAAGCAGTTCTACCGCGCTAGAATGAATGCCTTGGACCTGAATTTGAAGACCAAAGAGAGG ATTGTGGAGGAGGCCAATAAAGCCTTTGAATATAACATGCAGATATTCAGTGAACTGGACCAGGCTGGCT CCATGCTAGCAAGAGAAACCCTGGAGGATGGGCTCCCGGTACATGATGGGAAGGGAGATATACGTAAATG CCCCTTTTATGCTGCTCAGCCAGACAAAGGTACACTAGGAGGCAGCAACTGCCCCTTCCAGACAACCGTG GCTGTGCTGAGGAAGCCTAGCCTGCAGCTCATTCTGGCTGCCAGTGTGGCCTTGGTAGCTGGACTCTTGG CCTGGTACTACATGTGAAGGACCTGTCATGTTGTTTGCATCCTACCCCAAGTGACCTGTTCTATCCCCAT CTCCACCTCTGCCTAAAACTACCACCTCAGGTGACTTTTTTTTAATGCTGGGTTTGAGAAAACAAGCAAC CAATAAAAGCCAGACACTAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3484259 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3484259 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11207 same embryo
	EMAGE:11206 same embryo
	EMAGE:11209 same embryo
	EMAGE:11204 same embryo
	EMAGE:11208 same embryo
	EurExpress:euxassay_003408 same experiment
	MGI:4825398 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source