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EMAGE:11206

Rps7 ribosomal protein S7 ( MGI:1333818)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11206 EMAGE:11206 EMAGE:11206 EMAGE:11206 EMAGE:11206
"Pseudo-wholemount" of euxassay_003416. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003416_01 euxassay_003416_02 euxassay_003416_03 euxassay_003416_04
EMAGE:11206 EMAGE:11206 EMAGE:11206 EMAGE:11206 EMAGE:11206
euxassay_003416_05 euxassay_003416_06 euxassay_003416_07 euxassay_003416_08 euxassay_003416_09
EMAGE:11206 EMAGE:11206 EMAGE:11206 EMAGE:11206 EMAGE:11206
euxassay_003416_10 euxassay_003416_11 euxassay_003416_12 euxassay_003416_13 euxassay_003416_14
EMAGE:11206 EMAGE:11206 EMAGE:11206 EMAGE:11206 EMAGE:11206
euxassay_003416_15 euxassay_003416_16 euxassay_003416_17 euxassay_003416_18 euxassay_003416_19
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euxassay_003416_20 euxassay_003416_21 euxassay_003416_22 euxassay_003416_23 euxassay_003416_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11206Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11206_wholemount_strong.wlz
11206_wholemount_moderate.wlz
11206_wholemount_weak.wlz
11206_wholemount_possible.wlz
11206_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11206_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5952
Entity Detected:Rps7, ribosomal protein S7 ( MGI:1333818)
Sequence:sense strand is shown

>T5952
CCCACGCGTCCGGTCAGGGTGAAGCCATGTTCAGCTCGAGCGCCAAGATCGTGAAGCCCAATGGCGAGAA
GCCGGACGAGTTCGAGTCTGGCATCTCTCAGGCGCTGCTCGAGCTGGAGATGAACTCCGATCTCAAGGCG
CAGCTGCGTGAACTCAACATCACCGCAGCCAAGGAAATCGAAGTTGGTGGTGGTCGGAAAGCTATCATAA
TTTTTGTACCAGTTCCTCAGCTTAAATCTTTCCAGAAAATCCAAGTCCGGCTAGTTCGTGAATTGGAGAA
AAAGTTCAGTGGCAAGCACGTGGTCTTCATTGCTCAGAGGAGGATTCTGCCCAAGCCAACCCGGAAAAGC
CGTACGAAAAATAAGCAGAAGCGCCCCAGAAGCCGTACCCTGACAGCAGTGCACGACGCCATCCTTGAGG
ACTTAGTCTTCCCAAGTGAAATTGTGGGTAAGAGGATCCGTGTGAAACTGGATGGCAGCCGGCTCATAAA
AGTTCACTTAGACAAAGCACAGCAGAACAACGTGGAACACAAGGTTGAAACTTTTTCTGGTGTGTACAAG
AAGCTCACAGGCAAGGATGTTAATTTTGAATTCCCAGAGTTTCAGTTGTAAAGAAAATGACTGAATAAAG
TGTCATTCATAGTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3484169 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3484169 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11207 same embryo
 EMAGE:11205 same embryo
 EMAGE:11209 same embryo
 EMAGE:11204 same embryo
 EMAGE:11208 same embryo
 EurExpress:euxassay_003416 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS