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EMAGE:11208

Parp8 poly (ADP-ribose) polymerase family, member 8 ( MGI:1098713)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11208 EMAGE:11208 EMAGE:11208 EMAGE:11208 EMAGE:11208
"Pseudo-wholemount" of euxassay_003449. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003449_01 euxassay_003449_02 euxassay_003449_03 euxassay_003449_04
EMAGE:11208 EMAGE:11208 EMAGE:11208 EMAGE:11208 EMAGE:11208
euxassay_003449_05 euxassay_003449_06 euxassay_003449_07 euxassay_003449_08 euxassay_003449_09
EMAGE:11208 EMAGE:11208 EMAGE:11208 EMAGE:11208 EMAGE:11208
euxassay_003449_10 euxassay_003449_11 euxassay_003449_12 euxassay_003449_13 euxassay_003449_14
EMAGE:11208 EMAGE:11208 EMAGE:11208 EMAGE:11208 EMAGE:11208
euxassay_003449_15 euxassay_003449_16 euxassay_003449_17 euxassay_003449_18 euxassay_003449_19
EMAGE:11208 EMAGE:11208 EMAGE:11208 EMAGE:11208 EMAGE:11208
euxassay_003449_20 euxassay_003449_21 euxassay_003449_22 euxassay_003449_23 euxassay_003449_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11208Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11208_wholemount_strong.wlz
11208_wholemount_moderate.wlz
11208_wholemount_weak.wlz
11208_wholemount_possible.wlz
11208_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11208_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cervical intervertebral disc
moderate moderate
regionalmoderate expression: see section 12 13 14 15
lumbar intervertebral disc
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17
thoracic intervertebral disc
moderate moderate
regionalmoderate expression: see section 13 14 15 16
limb
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 24
hindlimb
moderate moderate
regionalmoderate expression: see section 22 23
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
thymus primordium
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16
visceral organ system
moderate moderate
regionalmoderate expression: see section 05
central nervous system
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
adenohypophysis
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16
vibrissa
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 21 22 23
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 08 18 19
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 18 19 20 21
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 09 10
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 07 08 18 19
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 11 12 17
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 09 10 11 12 17 18 19
not examined not examined
regionalnot examined expression: see section 01 02 03 04 23 24
neural retina
moderate moderate
regionalmoderate expression: see section 24 weak expression: see section 01 02 03
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 15 16 17 18 19 20 21
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 11 12 13 15 16 17
heart
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
esophagus
moderate moderate
regionalmoderate expression: see section 13 14
tongue
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16 17
stomach
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12
midgut
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 11 12 15 16
lower jaw molar
moderate moderate
regionalmoderate expression: see section 06 07 19 20
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 11 12 16
upper jaw molar
moderate moderate
regionalmoderate expression: see section 06 07 19 20
liver
moderate moderate
single cellmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
bladder
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17
metanephros
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 18 19 20 21 22 23
genital tubercle of male
moderate moderate
regionalmoderate expression: see section 14 15 16 17 18
lung
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
trachea
moderate moderate
regionalmoderate expression: see section 14
not examined not examined
regionalnot examined expression: see section 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6809
Entity Detected:Parp8, poly (ADP-ribose) polymerase family, member 8 ( MGI:1098713)
Sequence:sense strand is shown

>T6809
CGGACGCGTGGGTTCAGCCCCTGCCTCGGCCAGAGGTTTCATTTTTAACTGAATATTTACGAAAGCTGGA
AGCGTGCGAGGGGGGTGGGGTGGAGTGGAAATAGCGGCTGCTTCTTTTCCAGGGATTTATTTAATGGGGA
TGTGTTCAAGGCAAGAGAGAATTCAGAAGGATATCGACGTCGTGATCCAGAAGTCAAGAGCCGAGAAAGA
CTGCCTGTTTGCAGATTTCAGATACTCAGACTCCACCTTTACTTTCACCTACGTTGGCGGCCCCAAAAGT
GTATCCTACTCCGTCCACGTCTCAGAAGACTACCCAGACAACACGTATGTGTCAAGTTCAGAAAATGATG
AGGATGTCCTAGTCACTACAGACCCAATTCCTGTCATTTTCCATAGAATAGCAACAGAATTAAGGAAAAC
AAATGATATTAACTGTTGTTTGTCCATAAGATCTAAGTTACAAAAGGAAAATGGGGAGGAGTCAAGGCAA
AATAGTACGGTGGAAGAAGACTCAGAGGGTGACAACGACTCTGAAGAATTTTATTATGGAGGACAGGTGA
ACTATGATGGGGAACTACACAAGCACCCTCAGCTAGAAGCTGATCTGTCTGCGGTGAGAGAGCTCTATGG
ACCACATGCAGTTTCTCTCAGGGAATATGGGGCTATCGATGATGTAGATATTGATCTGCACATTGATGTC
AGCTTTCTGGATGAGGAGATTGCTGTGGCCTGGGAAGTGATTAGAACAGAACCTATTATTGTCCGACTAC
ACTGCTCTCTTACACAGTATTTAAACGGCCCAGTGCCCACGGTTGATGTCTTTCAGATTTCGACAAAAGA
GCGGTTTGGATTGGGACATCAACTGAAAAAAATCATGCAGACGTTTGTTTCTCAGCAGTGGAAACAGAGC
AAAGACAAGTCCAATTGCCCGCACGGTAAGAAGCTGTCAGAGAAGAAGGTGAAGTCACCTCTGCATTTGT
TTTCTACCCTGCGCAGGTCGCCCAGCTATCCTCCCCCTGGTTGTGGCAAAAGCAAATCCAAACTGAAACC
CGAACAAGACGGCATCTCCAAAACACACAAGCTGCTGCGGAGGACTTGCTCCAGTACTGTCAAAGCCGAT
GAGATGTGTGCCAAGTCGCACAGGACCTTCGGGCGCTCGCTGTCCAGCGACCCCAGGGCCGAGCAGGCGA
TGTCCACCATTAAATCGCACAAACTTTTGGGCCGGCCCTGCCCCTCAGCCGGCAAGCAAGAGGACTGCCT
CACACTGAAGTCCCATAAGTTATTGACTCGATCTTGTTCTGGAGACCCACGATGTGAGCACAACACCAAC
TTGAAGCCCCACAAACTGCTAAGCAGATCTTACTCCAGTAATCTCAGAATGGAAGAGTTATATGGCCTGA
AAAATCACAAATTGCTCAGCAAGTCTTACTCCAGTGCCCCCAAGACATCGAAAATGGAGCATTTCAAGGA
ACCCAACGCAGAGGGCAGGAGGCTCTCGCTTACCTCAGGGCTTATCGGTATTTTAACACCATCATCGTCT
TCTTCCCAGCCTCCTACAAATGGTGCAAAAAGCATTCCAATCCGAGACCGCGGCTTCCTGGTGCAGACCA
TTGAGTTTGCTGAGCAGCGGATCCCTGTACTGAATGAATATTGTGTGGTGTGTGATGAACCACACGTGTT
TCAAAATGGCCCCATGCTTAGGCCCACAGTATGTGAGCGAGAGCTGTGTGTGTTTGCTTTTCAAACCCTG
GGAGTGATGAATGAAGCTGCCGACGAGATCGCTACCGGAGCTCAGGTGGTAGATCTGCTTGTGTCCATGT
GTAGATCTGCGTTGGAATCTCCTAGAAAAGTTGTCATTTTTGAGCCATATCCTTCTGTGGTAGATCCTAA
TGACCCTCAGATGTTAGCCTTCAATCCCAGGAAGAAGAACTATGATCGTGTAATGAAAGCTCTGGACAGC
ATAACTTCCATCAGAGAAATGACACAGGCACCCTATCTGGAAATCAAGAAGCAGATGGATAAACAGGACC
CCCTTGCTCATCCACTTCTGCAATGGGTCATCTCAAGTAATAGGTCACATATTGTGAAACTGCCAGTCAA
CAGGCAACTGAAGTTCATGCACACGCCACACCAGTTCCTCCTCCTCAGCAGCCCACCAGCCAAAGAGTCC
AACTTCAGAGCCGCCAAGAAGCTCTTCGGGAGCACCTTCGCATTTCATGGCTCTCACATTGAAAACTGGC
ACTCTATCCTGAGGAATGGTCTGGTCGTTGCCTCTAACACGCGTCTGCAGCTCCACGGTGCAATGTACGG
AAGTGGGATCTATCTTAGCCCGATGTCCAGCATATCATTTGGTTACTCAGGGATGAACAAGAAGCAGAAG
GTGTCAAGCAAGGACGAGCCAGCTTCCAGTAGTAAAAGCAGCAATGCATCACAGTCTCAGAAAAAAGGAC
AGCAATCCCAGTTCCTGCAAAGCCGTAACTTGAAATGCATAGCCTTATGTGAAGTGATCACGTCACCTGA
CCTGCACAAACATGGCGAGATATGGGTTGTCCCGAACACGGATCATGTCTGTACAAGATTCTTTTTTGTT
TATGAAGATGGCCAGGTGGGAGATGCCAATATCAACACACAAGAAGGAGGTATTCACAAAGAGATACTCC
GTGTGATTGGTAATCAGACTGCGACTGGTTAAAGGAAGACCACGTAACAGGATCTGAAAAGCCTTCCTCG
GGTTCAAAGCTGGATTTTGACTGAAGAAGATGACAAAATAATTTATTGTTATTATAAACAAAATTAACCC
TTTGAATACTGATTTTTTTTTCTTTTCTTAGTATTTCTAAATATTTCATTAAATGCCTAAAATGGTATAA
GAAAAAAAAAAAAAAAG
Notes:The probe template was PCR amplified from IMAGE:3710450 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3710450 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11207 same embryo
 EMAGE:11205 same embryo
 EMAGE:11206 same embryo
 EMAGE:11209 same embryo
 EMAGE:11204 same embryo
 EurExpress:euxassay_003449 same experiment
 MGI:4827054 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS