EMAGE mouse gene expression spatial database

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EMAGE:11254

Nme6 non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase) ( MGI:1861676)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006508. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006508_01	euxassay_006508_02	euxassay_006508_03	euxassay_006508_04

euxassay_006508_05	euxassay_006508_06	euxassay_006508_07	euxassay_006508_08	euxassay_006508_09

euxassay_006508_10	euxassay_006508_11	euxassay_006508_12	euxassay_006508_13	euxassay_006508_14

euxassay_006508_15	euxassay_006508_16	euxassay_006508_17	euxassay_006508_18	euxassay_006508_19

euxassay_006508_20	euxassay_006508_21	euxassay_006508_22	euxassay_006508_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11254_wholemount_strong.wlz

11254_wholemount_moderate.wlz

11254_wholemount_weak.wlz

11254_wholemount_possible.wlz

11254_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11254_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6587
Entity Detected:	Nme6, non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase) ( MGI:1861676)
Sequence:	sense strand is shown >T6587 GGCGGGAGTACCAGATCGCCGGTCCTCCCAGGAGACCCCTTAGCAGCCTGGCCTTGGAATGGCTAGATCA GGTTCATGCACTGATTCATCCAGCTAAGATCTGTGGAAATCCAGGGCAGAGATGACCTCCATCTTGCGAA GTCCCCAAGCTCTTCAGCTCACACTAGCCCTGATCAAGCCTGATGCAGTTGCCCACCCACTGATCCTGGA GGCTGTTCATCAGCAGATTCTGAGCAACAAGTTCCTCATTGTACGAACCAGGGAACTGCAGTGGAAGCTG GAGGACTGCCGGAGGTTTTACCGAGAGCATGAAGGGCGTTTTTTCTATCAGCGGCTGGTGGAGTTCATGA CAAGTGGGCCAATCCGAGCCTATATCCTTGCCCACAAAGATGCCATCCAACTTTGGAGGACACTGATGGG ACCCACCAGAGTATTTCGAGCACGCTATATAGCCCCAGATTCAATTCGTGGAAGTTTGGGCCTCACTGAC ACCCGAAATACTACCCATGGCTCAGACTCCGTGGTTTCCGCCAGCAGAGAGATTGCAGCCTTCTTCCCTG ACTTCAGTGAACAGCGCTGGTATGAGGAGGAGGAACCCCAGCTGCGGTGTGGTCCTGTGCACTACAGTCC AGAGGAAGGCATCCACTGTGCAGCTGGAACAGGAGGCCACAAACAACCTAACAAAGCCTAGGAGACCCAG CCGAGTAAAGTCCCGAGGACTGGTCGCAGTGTTCAGCCCTCCCTCCTAGGGTCAGGGAAGGTGGTCTGAA GGCACTGTCCCTGTTGGACCCCTCCATGAGCTGGCGAGTCTAGCCGACCTCAGCTCAGCTGCTGCAGTCC AGCTCTATCTACCTCTTCTGTGGAGTACTCATGGGTGTTCAGGAAGGATGGTGACGCCTCTTTTTACAAA GGACTATCCTCCTTCCTCAAGAAAGAGCCTGCCTGTTGACTAGTCAGGAAAATGCAGTGCCTGGGTAACC AGCATCCGACCTGGCTTCCTTAATAAACTCTGCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3593804 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3593804 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11255 same embryo
	EMAGE:11257 same embryo
	EMAGE:11253 same embryo
	EMAGE:11256 same embryo
	EurExpress:euxassay_006508 same experiment
	MGI:4826732 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source