EMAGE mouse gene expression spatial database

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EMAGE:11267

Tfg Trk-fused gene ( MGI:1338041)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006653. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006653_01	euxassay_006653_02	euxassay_006653_03	euxassay_006653_04

euxassay_006653_05	euxassay_006653_06	euxassay_006653_07	euxassay_006653_08	euxassay_006653_09

euxassay_006653_10	euxassay_006653_11	euxassay_006653_12	euxassay_006653_13	euxassay_006653_14

euxassay_006653_15	euxassay_006653_16	euxassay_006653_17	euxassay_006653_18	euxassay_006653_19

euxassay_006653_20	euxassay_006653_21	euxassay_006653_22	euxassay_006653_23	euxassay_006653_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11267_wholemount_strong.wlz

11267_wholemount_moderate.wlz

11267_wholemount_weak.wlz

11267_wholemount_possible.wlz

11267_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11267_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7094
Entity Detected:	Tfg, Trk-fused gene ( MGI:1338041)
Sequence:	sense strand is shown >T7094 GCAGCGGCCGGGTCAGCTGAGGTGGACGGCCTCGGCAAGCGGGTTTTTTTTCCCCTAGAGTTGTATATAT AGAGCATCCTGGACTCCACAATGAACGGACAGTTGGACCTAAGTGGGAAGCTGATTATCAAAGCTCAACT TGGGGAAGATATCCGACGAATTCCCATTCATAATGAAGACATTACTTACGATGAATTAGTGCTAATGATG CAGCGAGTATTCAGAGGAAAGCTTCTGAGTAATGATGAAGTTACAATAAAGTATAAGGATGAAGATGGAG ATCTTATAACAATTTTTGATAGTTCTGACCTTTCCTTTGCAATTCAGTGTAGTAGAATACTGAAACTGAC ATTATTTGTTAATGGCCAGCCAAGACCCCTTGAATCAAGTCAGGTGAAATATCTTCGTCGAGAACTGGTA GAACTTCGAAATAAAGTGAATCGCTTATTGGATAGCTTAGAACCACCTGGAGAACCAGGACCTTCCACCA GTATTCCTGAAAATGATACTGTGGATGGCAGGGAAGAGAAGCCTGCCGCTTCTGACTCTTCTGGGAAACA GTCAACTCAGGTTATGGCAGCAAGTATGTCAGCTTTTGATCCTCTGAAAAACCAAGATGAAATCAACAAA AACGTCATGTCAGCATTTGGATTAACAGATGACCAGGTTTCAGGCCCACCCAGTGCCCCTACAGAAGACC GCTCCGGAACACCTGACAGCATTGCTTCTTCCTCCTCAGCAGCACACCCAGCAGTTCAGCCGCAGCAACC TCCCTATGCAGGAGCTCAGACACAAGCAGGTCAGATTGAAGGTCAGATGTACCAGCAGTACCAGCAGCAG GCTGGCTACAGTGCCCAGCAGCCTCAGGCCCCTCCTCAGCAGTATGGTGTTCAGTACTCAGCTAGTTACA GCCAGCAGACTGGACCCCAACAACCTCAGCAGTTCCAGGGATATGGCCAACAACCAACTTCCCAGGCACC GGCTCCTGCCTTTTCTGGCCAGCCACAACAACTGCCTGCTCAACCACCACAGCAGTACCAGGCGAGCACT TACCCTCCACAAACGTATACTACCCAACCATCTCAGCCTGCCAACTATACTGTTCCCCCTGGCTCTCAGC CTGGAATGGCTCCAAGCCAACCTGGTGCTTACCAACCAAGACCAGGTTTTACGCCATCTCCTGCAAGTAC CATGACCCCTCCTTCTAGTGGGGCTAACCCTTATGCTCGTAACCGTCCTCCTTTTGGTCAGGGGTATGCC CAACCTGGACCTGGTTATCGATAAGGAGGTTCAGCCACACTAATAATAATGGCTGATTGCCTCCCAAAAG ACTATACAATACTATTTTGTTTCAATTTGTATTGTATTGAAGTTTAAAAATTTAAAAAGCAGAGCATTTT TTTATGATATCATTGTTGCTGTTAATTGAAAATATAATTTACCAGAATACAGACACAAAAAGACCAAAAT GAATATTGTTTCCTCCCCTGCTTAAAATTGTAGCCGCTTCCTAGTTATTTTGGAACACTACTCTTACATG CGTGTAAAATGATTGGCTTTCTAGTTTCCCAGAGAATATTAAAAAGCTAGGCTAAGGTATAGCCATCACA CTTGGCTATTTACTGTTAACATGATGTACTAAAAATAGAGCCCTCTGAGAAGATGACTAGACATTATGTA TAAAATGTAACATTGATAGGCTAATAAAGGTGATTGAATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4015300 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4015300 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11270 same embryo
	EMAGE:11269 same embryo
	EMAGE:11268 same embryo
	EurExpress:euxassay_006653 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source