EMAGE mouse gene expression spatial database

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EMAGE:11269

Dnajc14 DnaJ (Hsp40) homolog, subfamily C, member 14 ( MGI:1921580)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006633. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006633_01	euxassay_006633_02	euxassay_006633_03	euxassay_006633_04

euxassay_006633_05	euxassay_006633_06	euxassay_006633_07	euxassay_006633_08	euxassay_006633_09

euxassay_006633_10	euxassay_006633_11	euxassay_006633_12	euxassay_006633_13	euxassay_006633_14

euxassay_006633_15	euxassay_006633_16	euxassay_006633_17	euxassay_006633_18	euxassay_006633_19

euxassay_006633_20	euxassay_006633_21	euxassay_006633_22	euxassay_006633_23	euxassay_006633_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11269_wholemount_strong.wlz

11269_wholemount_moderate.wlz

11269_wholemount_weak.wlz

11269_wholemount_possible.wlz

11269_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11269_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7101
Entity Detected:	Dnajc14, DnaJ (Hsp40) homolog, subfamily C, member 14 ( MGI:1921580)
Sequence:	sense strand is shown >T7101 GCGGGAGGCGGAGCCGGGGGAGCTGCTCCTGTAGCTCCCCCTGGCAGCTCCCCCTGGCCTCAGGCGCAGC TGCTTGAGGAGGGAGGAGTTACCGCTGCTGCTCGGCAATGATGGCAGTGACCTAAAATCCTCAGGAAGCC CGGGGTCATGGCCCAGAAGCACCCGGGAGAAAGAAGGTTGTGTGGAGCCCACCGCAGTGGTGGTACCTCC CTCAGTACATCAGGATCCTCTGTGGACCCCGAAATACTTTCATTCTCAGGACTCAGGGACTCAGCAGAGA CTGCTCCTAATGGTACACGATGCCTCAAAGAGCACTCCGGCCCTAAGTACACACAGCCTCCAAATCCAGC CCACTGGTCAGATCCAAGCCATGGTCCTCCAAGGGGTCCAGGACCACCTAGAGGAGGAGGCTACCCTGAT GAGAGCGAGACAGGTTCAGAAGAGTCAGGAGTGGACCAGGAACTCTCAAGAGAGAATGAGACTGGGTACC AGGAGGATGGGAGTCCTTCTTTTCTTTCCATTCCATCTGCTTGTAACTGCCAGGGAAGCCCTGGAGTCCC CGAAGGAACTTACTCCGAGGAAGGAGATGGCTCTTCTAGCAGCCTTTGCCACCATTGCACCTCTCCAGCC TTGGGGGAAGATGAAGAGTTGGAAGAAGAATATGATGATGAGGAACCTCTTAAATTTCCCAGTGATTTTT CACGCGTGTCCAGTGGAAAGAAACCCTTGTCCCGGAGGCAGAAGCACCGTTTTCCGATCAAGGAGGATGT TCGGGATAGTGGACGCAGAGAACCCAAGGCCCCAGGTCGTCATCGGCTGGCCCGAAAAAGAAGTCAGACA GATAAGCGCAGAGGCCTGGGACTGTGGGGAGTAGAGGAACTATGTCAGCTTGGACAAGCAGGCTTCTGGT GGCTGATTGAACTTCTGGTATTGGTGGGAGAGTATGTGGAAACCTGTGGCCATCTCATTTACGCATGCAG GAAGCTGAAAGGCAGTGACCTGGACCTTTTTCGAGTCTGGGTGGGGGTCTGGGCCAGGAGACTGGGGGGC TGGGCCCGCATGATGTTCCAGTTTCTAAGCCAGAGCTTCTTCTGTGTGGTGGGGCTGCTCATCCGTATTC TTAGGGTAGTGGGTGCTTTCCTACTCCTGGCTCTCGCCCTCTTTTTGGGATGTCTACAATTGGGCTGGAG GTTTTCGGTGGGACTGGGCAACCGGTTAGGCTGGAGGGATAAGACCGCTTGGCTGTTCTCTTGGCTGGGT TCTCCAGCCTTGCATCATTGCTTGACTCTGCTGAAAGATAGCAGGCCATGGCAGCAGCTGGTAAGGTTAA TACAGTGGGGCTGGCAGGAGCTGCCCTGGGTCAAACAGAGGACTAAGAAACAGGGCAATGCACCTGTAGC TAGCGGGCGATACTGCCAGCCTGAAGAGGAAGTGACCCGACTCTTGACCATGGCTGGGGTTCCTGAAGAT GAACTAAACCCTTTTCATGTGCTGGGGGTTGAAGCTACAGCATCCGACACTGAACTAAAGAAGGCCTATA GGCAGCTAGCAGTAATGGTCCATCCTGATAAAAATCACCATCCCCGGGCTGAGGAGGCCTTCAAAATTTT GCGGGCAGCTTGGGACATTGTCAGCAACCCAGAGAGGCGGAAGGAATATGAGATGAAACGGATGGCAGAG AATGAGCTCAGCCGGTCAGTGAATGAGTTTCTGTCGAAACTACAAGATGACCTCAAGGAGGCAATGAACA CGATGATGTGCAGCAGATGCCAAGGAAAGCATAGGAGGTTTGAAATGGACCGGGAACCCAAGAGTGCCAG ATACTGTGCTGAGTGTAACAGGCTGCATCCTGCTGAGGAAGGAGACTTTTGGGCAGAGTCGAGCATGCTG GGCCTCAAGATCACTTACTTTGCGCTGATGGATGGAAAGGTGTATGACATTACAGAGTGGGCTGGATGCC AGCGTGTGGGTATCTCCCCAGATACTCACAGAGTTCCTTACCACATCTCATTTGGTTCTCGGGTACCCGG CACCAGTGGCCGGCAGAGGGCCACTCCAGAGTCCCCTCCTGTTGACCTGCAGGATTTCTTGAGCCGGATC TTTCAAGTACCTCCGGGGCCGATGTCCAATGGGAACTTCTTTGCCGCACCTCACCCTGGCCCTGGGACCA CTTCGACCTCTAGGCCCAACAGTTCAGTACCCAAGGGAGAAGCCAAACCTAAACGGCGGAAGAAAGTGAG GCGGCCCTTTCAACGATGACACCCCTTCTCTTCCTTCCTCAAATCAATGTCAGGGAGTCAAAAGGGCTGT GTACAGCACAGGATGGAATTTGATTTATTTTAAAATACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4016209 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4016209 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11270 same embryo
	EMAGE:11267 same embryo
	EMAGE:11268 same embryo
	EurExpress:euxassay_006633 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source