EMAGE mouse gene expression spatial database

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EMAGE:11288

Ube2a ubiquitin-conjugating enzyme E2A, RAD6 homolog (S. cerevisiae) ( MGI:102959)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006706. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006706_01	euxassay_006706_02	euxassay_006706_03	euxassay_006706_04

euxassay_006706_05	euxassay_006706_06	euxassay_006706_07	euxassay_006706_08	euxassay_006706_09

euxassay_006706_10	euxassay_006706_11	euxassay_006706_12	euxassay_006706_13	euxassay_006706_14

euxassay_006706_15	euxassay_006706_16	euxassay_006706_17	euxassay_006706_18	euxassay_006706_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11288_wholemount_strong.wlz

11288_wholemount_moderate.wlz

11288_wholemount_weak.wlz

11288_wholemount_possible.wlz

11288_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11288_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus

moderate

regional

moderate expression: see section 07 08 09 10 11 weak expression: see section 12

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 weak expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7069
Entity Detected:	Ube2a, ubiquitin-conjugating enzyme E2A, RAD6 homolog (S. cerevisiae) ( MGI:102959)
Sequence:	sense strand is shown >T7069 CCACGCGTCCGGCGGCAGTGCCGTCCCTCTCGGCGCCTCCTCGGCTTCTCCGGCTTCCTCGGCTTCCTCG GGGACCGCGGACCCTGGTATATGCCCGACTGCTGAGCCCGCTAAAGCCATGTCGACCCCGGCCCGGCGCC GCCTCATGAGGGACTTCAAGAGGTTACAGGAAGATCCTCCGGCCGGAGTGAGCGGGGCTCCGTCGGAGAA CAACATAATGGTTTGGAATGCGGTCATTTTTGGGCCCGAAGGGACCCCGTTTGAGGATGGAACATTTAAG CTTACAATTGAATTCACTGAAGAGTATCCTAATAAGCCACCTACTGTTAGATTTGTCTCTAAGATGTTCC ATCCTAACGTCTATGCAGATGGTAGTATATGTCTGGATATACTTCAGAACCGTTGGAGTCCAACCTATGA TGTGTCTTCCATTTTAACGTCCATACAGTCTCTATTGGATGAACCTAATCCCAATAGTCCAGCAAACAGC CAGGCTGCCCAGCTGTACCAGGAGAACAAGCGGGAATATGAAAAGCGTGTTTCTGCAATAGTAGAACAAA GCTGGCGCGACTGTTGACTCAGGGTAGAGCAGAAGAAGAGGCTGGCCATAAGAAAAATGTATATTGATGC ATTTATCACCTTATTCCTAATTCATTACATTTACTTTATTAAAAACAAAATAGCTGTTGTGCTGTTTCTG TCTTCCCTTGGCAAGTTTTTCTTCCCCTTTCTGTCCTCCTGTGGAGCATCAGAGATATTATTTGAAATCC AATGTACTGTACCTGGGTTACTTGCAAACATTACAATTGCATATGTCCCTTTGTTGTATCTCATCTCCAG CCTCAAAGCAGTTATCAGGTTACTGTACTTTTTATACTAAGCTTTTAAGATAAAATATTATGTATAGTCT GGTGACCAGGATGTTATTTGTAACAAAATGATTGCTGCTGTGTTTCATCCTGGCCATTCCTTCCCTTGTA TTTGATTTGGCAAAGAATGTATTTAGAATATGACCTAAAGAAAACAGGAATACATTCATATAAACAATAT TTGAAAGGGGAAGGAAAGCTGAAAAACCCTATACTCATTTGTAAGCAATATGAACATGAACTACAAGATG GCACTTACTGGTAGCAAGGAGACTGCTAAAGGACCCAGCTAACAAACTGAAGCAATTTTAATAACTGACA CAGTCCACTTTGTGCGAGATATTCATAGTGTAAGCGCATTCTGTTTCGGAATTCTGGCCTTCAGAGTTGT TTTTTATAGAGGTGTAAGAACAGGACTAGACTTTTTTTTAAAGTTAGGAAAAACCGAAGATCCTCTATTC TCTTAAAGGAGCAGTGTTGCCCTAAATGAACTTTCCTTTTTATGATTCACGGAAGGGAGTGTATCTTGCA AACTTTTCCCATTCAAATCCTGAGTTAATGCTGCATGCTTTCAGTGTTTTGAGAACCTTCTATTGCCCAT ATCTGCAATTTTTTCTTTGAATATTTACACTTTTAAATATACAGTATGGGGCAAGGCTTGTAAATGTTTT GTCTAATATATTTTCATTGTTAATCTATTATGCATTTATAGCATTTCTAACTTTGCACAAGTAACCCATG TAAAAACTGTACATTTTCAAAAGTTGTAAATAAAAAAATAACCTTAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4012075 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4012075 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11285 same embryo
	EMAGE:11287 same embryo
	EMAGE:11286 same embryo
	EurExpress:euxassay_006706 same experiment
	MGI:4829050 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source