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EMAGE:11295

Tubb5 tubulin, beta 5 class I ( MGI:107812)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11295 EMAGE:11295 EMAGE:11295 EMAGE:11295 EMAGE:11295
"Pseudo-wholemount" of euxassay_003649. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003649_01 euxassay_003649_02 euxassay_003649_03 euxassay_003649_04
EMAGE:11295 EMAGE:11295 EMAGE:11295 EMAGE:11295 EMAGE:11295
euxassay_003649_05 euxassay_003649_06 euxassay_003649_07 euxassay_003649_08 euxassay_003649_09
EMAGE:11295 EMAGE:11295 EMAGE:11295 EMAGE:11295 EMAGE:11295
euxassay_003649_10 euxassay_003649_11 euxassay_003649_12 euxassay_003649_13 euxassay_003649_14
EMAGE:11295 EMAGE:11295 EMAGE:11295 EMAGE:11295 EMAGE:11295
euxassay_003649_15 euxassay_003649_16 euxassay_003649_17 euxassay_003649_18 euxassay_003649_19
EMAGE:11295 EMAGE:11295 EMAGE:11295 EMAGE:11295
euxassay_003649_20 euxassay_003649_21 euxassay_003649_22 euxassay_003649_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11295Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11295_wholemount_strong.wlz
11295_wholemount_moderate.wlz
11295_wholemount_weak.wlz
11295_wholemount_possible.wlz
11295_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11295_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6671
Entity Detected:Tubb5, tubulin, beta 5 class I ( MGI:107812)
Sequence:sense strand is shown

>T6671
CTTGCATCTCGCTGCTGCAGCCTCCCGGGCTCCGGTCCATCCTTTCAGTCTGAGACCGGCCCAGAAGAAA
AAAACCCCAAAAACCTTAATTTTCTTTCTTGTTCGGTACCTACATTGGAACCACCAAAAACAATTATTTC
AGTAAACCGTAGCCATGAGGGAAATCGTGCACATCCAGGCCGGACAGTGTGGCAACCAGATCGGTGCTAA
GTTCTGGGAGGTGATAAGCGATGAACATGGCATCGACCCCACCGGTACCTACCACGGTGACAGCGACCTG
CAGCTGGACCGAATCTCTGTGTACTATAATGAAGCCACAGGTGGCAAGTATGTCCCTCGAGCTATCTTGG
TGGATCTAGAACCTGGGACTATGGACTCCGTTCGCTCAGGTCCTTTTGGCCAGATCTTCAGACCAGACAA
CTTCGTTTTCGGTCAGTCTGGGGCAGGCAACAACTGGGCTAAAGGCCACTACACAGAGGGAGCTGAGTTG
GTTGACTCTGTCTTGGATGTGGTGCGGAAGGAGGCGGAGAGCTGTGATTGCCTGCAAGGCTTTCAGCTGA
CCCACTCACTGGGTGGAGGCACTGGCTCTGGCATGGGCACCCTGCTCATCAGCAAGATCCGGGAAGAATA
TCCTGACCGTATCATGAATACCTTCAGTGTGGTGCCCTCGCCCAAAGTGTCTGATACCGTGGTCGAGCCC
TACAATGCCACCCTGTCTGTCCATCAGTTGGTTGAGAACACGGATGAGACCTACTGCATCGACAACGAGG
CCCTCTACGACATCTGCTTCCGTACCCTCAAGCTCACCACGCCAACCTACGGAGACCTGAACCATCTCGT
CTCGGCCACCATGAGCGGCGTCACCACCTGCCTCCGTTTCCCGGGCCAGCTTAATGCTGACCTTCGAAAG
CTGGCTGTCAACATGGTGCCATTCCCACGTCTCCACTTCTTCATGCCTGGCTTTGCCCCTCTCACCAGCC
GTGGAAGCCAGCAGTACCGGGCCCTCACTGTGCCTGAACTTACCCAGCAGGTCTTCGATGCCAAGAACAT
GATGGCCGCCTGCGACCCGCGCCACGGCCGGTACCTCACAGTTGCCGCCGTCTTCCGTGGACGGATGTCC
ATGAAGGAGGTGGATGAGCAGATGCTCAACGTGCAGAACAAGAATAGCAGCTACTTCGTGGAATGGATCC
CCAACAATGTCAAGACAGCTGTCTGTGACATCCCACCACGTGGCCTCAAGATGGCAGTCACCTTCATTGG
AAACAGCACAGCCATCCAGGAGCTCTTCAAGCGCATCTCTGAGCAGTTTACGGCTATGTTCCGCCGGAAG
GCTTTCCTCCACTGGTATACGGGTGAGGGCATGGACGAGATGGAGTTCACCGAAGCTGAGAGCAACATGA
ACGACCTCGTCTCTGAGTACCAGCAGTACCAGGATGCCACCGCGGAAGAGGAAGAGGATTTCGGAGAGGA
GGCAGAAGAGGAGGCCTAAGGCAGAGAGCCCTGCATCAGCTCAGGCTGCTTAGATCCCTCAGCTTTCTCC
AACTGCCCTTTGTCCTCCAGTTTCTTTCTGCTGCCTCTGTCTTGTATTTGTTTTGTTCTGTTTTCTCATT
GGGGGTAAATGGTGCCTGGCACATGGCAGGCACTCAATAAATATTTGTTTGTGGAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3601177 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601177 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11298 same embryo
 EMAGE:11297 same embryo
 EMAGE:11296 same embryo
 EMAGE:11300 same embryo
 EMAGE:11299 same embryo
 EurExpress:euxassay_003649 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS