EMAGE mouse gene expression spatial database

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EMAGE:11301

Rtn4ip1 reticulon 4 interacting protein 1 ( MGI:2178759)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006551. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006551_01	euxassay_006551_02	euxassay_006551_03	euxassay_006551_04

euxassay_006551_05	euxassay_006551_06	euxassay_006551_07	euxassay_006551_08	euxassay_006551_09

euxassay_006551_10	euxassay_006551_11	euxassay_006551_12	euxassay_006551_13	euxassay_006551_14

euxassay_006551_15	euxassay_006551_16	euxassay_006551_17	euxassay_006551_18	euxassay_006551_19

euxassay_006551_20	euxassay_006551_21	euxassay_006551_22	euxassay_006551_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11301_wholemount_strong.wlz

11301_wholemount_moderate.wlz

11301_wholemount_weak.wlz

11301_wholemount_possible.wlz

11301_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11301_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1070
Entity Detected:	Rtn4ip1, reticulon 4 interacting protein 1 ( MGI:2178759)
Sequence:	sense strand is shown >T1070 TCGAGNCTGTTGGCCTACTGGATTGACCTGTGGGTTTGGCAGATTTTGGCTGAATTGAAATGTGTTAGTT TGACCTGCTTCAGGTCCTTTCTCTCCACCGTCGCACCCCCCACCCCCGCTCCTCCTTTCTTTTTTCTTTT TCTTTTTTTCTTTTCTTTTTTTCGAGGAAACGTTCTCCATCCCTGCTCAGGATGGGCGTTTTGAAGACCT GTGTACTTAGAAGAAGTGCTTGTGCCGCCGCCTGCTTCTGGAGAAGGACAGTTATCCCAAAGCCACCATT TAGAGGCATTAGTACTACGTCTGCGAGGAGCACTGTCATGCCCGCTTGGGTGATAGATAAATATGGAAAG AATGAAGTCCTTCGATTCACCCAGAACATGATGTTACCTATCATACACTATCCAAATGAAGTCATTATCA AAGTCCACGCTGCCAGTGTAAATCCTATAGATGTTAATATGAGAAGTGGTTATGGAGCCACAGCCCTAAA TATGAAGCGTGATCCCCTGCATATGAAAACCAAAGGAGAAGAGTTTCCTCTGACTCTGGGTCGGGATGTC TCT
Notes:	The probe template was PCR amplified from IMAGE:2099672 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2099672 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11305 same embryo
	EMAGE:11306 same embryo
	EMAGE:11302 same embryo
	EMAGE:11304 same embryo
	EMAGE:11303 same embryo
	EurExpress:euxassay_006551 same experiment
	MGI:4827831 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source