EMAGE mouse gene expression spatial database

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EMAGE:11304

Sdr9c7 4short chain dehydrogenase/reductase family 9C, member 7 ( MGI:1917311)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006552. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006552_01	euxassay_006552_02	euxassay_006552_03	euxassay_006552_04

euxassay_006552_05	euxassay_006552_06	euxassay_006552_07	euxassay_006552_08	euxassay_006552_09

euxassay_006552_10	euxassay_006552_11	euxassay_006552_12	euxassay_006552_13	euxassay_006552_14

euxassay_006552_15	euxassay_006552_16	euxassay_006552_17	euxassay_006552_18	euxassay_006552_19

euxassay_006552_20	euxassay_006552_21	euxassay_006552_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11304_wholemount_strong.wlz

11304_wholemount_moderate.wlz

11304_wholemount_weak.wlz

11304_wholemount_possible.wlz

11304_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11304_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7954
Entity Detected:	Sdr9c7, 4short chain dehydrogenase/reductase family 9C, member 7 ( MGI:1917311)
Sequence:	sense strand is shown >T7954 GCAAGTCTCAGAAGCTCTCCTTTCCTGTGGGTTCCTGTGGGCTGCCTGCTGGGCTCTGAGGATCAGCCTA TCCTACCATCTCCTGGGTCCATCCTCAGACATCAGCTCCCATGGCGGCCCTCACAGACTTTGCTTTTATG TACCGCTGGTTCAAAAATTGCAATCTGGTTAAAAACCTCTCTGAGAAGTATGTCTTCATCACTGGCTGTG ACTCCGGCTTTGGGAACCTGTTGGCCAAACAACTGGTTGATAGGGGCATGAAAGTGCTTGCTGCCTGCCT CACTGAGGAGGGGGCTCAGAAACTCCTGCAGGACACCTCCCACCAGCTGCAGACCTTCCTCCTAGATGTC ACCAAGTCGGAGAACGTCAAGGAAGCAGCCCAGTGGGTGAGGGACCAAGTGGGCGAGCAAGGCCTCTGGG CCCTGGTGAACAACGCTGGCGTGGGCCTGCCCAGTGGTCCCAACGAATGGCTGACCATAAAAGACTTTGT GAAAGTGATCAACATAAACCTGGTGGGGCTGATTGATGTCACTCTGAACATGCTGCCGATGATCAAGAAA GCCCGGGGCAGGGTGGTCAACATGTCCAGCTCTGGTGGCCGTGTGGCTATATTCGGCGGTGGTTACTGTG TCTCCAAGTTTGGTGTTGAAGCCTTCTCTGATAGCATCAGGCGCGAGCTTCATTTCTTCGGGGTGAAGGT CAGCATCATTGAGCCAGGGAACTACAAGACATCCATTCTGGGCCAAGAAGCTCTGGAATCACGCATGAAG AAGCTGTGGGATCGGCTGCCACAGGAGACCCGGGATAGCTACGGAGAGGAGTATTTCCAAACCTATACTA AAAAGTTGGTTAACTTGATGAGGTCAGCAGAGCCAAGAATCAGCGATGTCACCAACAGCATGGAGCACGC CATTGTCTCCCGGAGCCCCCGAATCCGCTACAACCCTGGCCTGGACGTCAAGTTTCTCTATCTCACCCTG GCTAAGCTGCCCACCCCTGTGACAGATTTCATCCTTAGCAGATACTTGCCAAGACCAGCAGACAGTGTTT GAGCTGGCGAGCTCAAGGGGTAGCCAGTGGAGCACAGGGGAGAGGGCAAAGGACTGGGGGATCACAAGAG GTGTTGTCATATATCTTGTGGGCAGAGAAGAGCATACTCAGCTCACTCCCACTGCAGGCTGATGTCTCCT TCCATCTGCTGGGGCCTCTAGAAACCTGATGTGACCCCGGCTGATGTAGATAGAGCGTTGCAGCATAGGC CGCAAGACACCCCTCACCAAGGCAGGTACTAGTGCTCCTGGACCTGAAAAGCCGGCTTCTGTTGACTGAC AAGCTGTCACCACCTCCGTAAAACACCATGGGACAATCTAGCCCACAGGAGAATCTCGGTCCCCTCCCCA CAGCTCTCAAAGCAGGCACTGGTTTGTGTATATATGGAATATGTAACATGGCAACAAGAACTGCCAGAGC ATACTCAGAGAAGGTTTTAAATAGTTTTCTTTTCTTTGCATTTCTCTATCTGATTGGGATCCATATAATG CTTGCTTCTCTAATCACCATTGATTCCTGTTTCAAGTATTTATTGAGCACTTACTACGTACCAGCACCAA AACTCTGGGGGTATGTGAGGGTGATGAAATCATGAACTTGTCTTGTTTTGTTTTGTTTTGTTTGTCTCAC GTTTTACTTTGTTACAGTAGCTAGCAGCAGAGATAGGACAGTGACCAAGGGGACAAAGATCAGAAAAAGG AAATTTGAAGGACCCTGGATCTCAATAGTCCTAGACTCGGATGTTTACTGGGATCACTCAGTTACTTAAA ATGCGTACGGAAGCTGGAGAGATGGCTCGGAGGTTAAGAGCATTTGTGCCTCGCAGAATCTGGAATAGAT CCCCAGTCTCCACACCGTGTCTCACAGCCTCCATAATTCCAGTTCCAGGGGATCGGATGCCCCCTTCTGA CCTCCACAGGTACCAAGCATGAATGTAATGTGTATTGATACATGCAGGCAAAAACACGCATACACATAAA ATAAGATAAATCTTTAAAAAGTGAATGTGTAAGGAAGCTGGGCATGGTATCTCATGTTTGTAATCCCAAC ATTTATGGGGCTAAGACAGGAGGATTTTTGTGAGTCTAAGGAAAGACTAGATTACAAAATGAGACACTGT CTCAAGAAACAAACCCCAGGAAACAAACAAAAGAACTCATAGGAACCCCAATGTTAGAAAATCCGATTCA ATAAACCTGAGTGAGGCAGAGCCCAGAACTATGAATTGTGTACTAAGGACCTAAATGTACCTGCGCACCT GGGAGGCAGTGATAGTCCCACAGGAGAAGCAGCCAATCAGCTCAGAGTGGGAAGTCATTTTCGCCAAGGC ACCCAGCTAAGTGGTCAAACAAACAGAACCCTTTTCCAGCAGCAGGTGATGAACTGTTTGTAACATCTTC AAATAAGTCATAGAACTAAATAATTCATGTCTTTAAAAATAGCTACCAACTTCTGAGTTATATGAACTAT TAATGAAAAAGGATACTTTTTTGATCAAGTTCATATACTGAATATCAAACCCAGAGCCTGAGCATCATAG ACAAGCACTCACCACTGACCTACATTCCCACCCTCAGATTTTCAATTAGCTTACTCAGAAGCTCAGGCTG GCCTTGAACTTGCTATAACTTTCAATATTCCCTCAAACTCATAATTCTCCTGCCTCTACTCCCAGAGTGC TATGACTACAGGCATGTGCCATCAGTCCCAACAATAACAGTGATAAAATAAATAAATAGAATTGTTTAAA ATTCTGCAGTTTGTGCAATAAAGGTCAAATATTATTATTTAAAAATCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30293068 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30293068 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11305 same embryo
	EMAGE:11306 same embryo
	EMAGE:11302 same embryo
	EMAGE:11301 same embryo
	EMAGE:11303 same embryo
	EurExpress:euxassay_006552 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source