EMAGE mouse gene expression spatial database

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EMAGE:11314

Hsd3b5 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5 ( MGI:104645)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006686. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006686_01	euxassay_006686_02	euxassay_006686_03	euxassay_006686_04

euxassay_006686_05	euxassay_006686_06	euxassay_006686_07	euxassay_006686_08	euxassay_006686_09

euxassay_006686_10	euxassay_006686_11	euxassay_006686_12	euxassay_006686_13	euxassay_006686_14

euxassay_006686_15	euxassay_006686_16	euxassay_006686_17	euxassay_006686_18	euxassay_006686_19

euxassay_006686_20	euxassay_006686_21	euxassay_006686_22	euxassay_006686_23	euxassay_006686_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11314_wholemount_strong.wlz

11314_wholemount_moderate.wlz

11314_wholemount_weak.wlz

11314_wholemount_possible.wlz

11314_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11314_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

strong

homogeneous

strong expression: see section 10 11 12

kidney calyx

strong

regional

strong expression: see section 10 moderate expression: see section 09 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7387
Entity Detected:	Hsd3b5, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5 ( MGI:104645)
Sequence:	sense strand is shown >T7387 CCACGCGTCCGGATCAAGAAGGGAACTGTGCCTGCCTTCAGGATCTAAAGCTCTTGGAAACAAAAGGAAC ACTTTATATCACATCTATATCCTCAACTATCTGATTCCTGTGTTGAACATGCCTGGATGGAGCTGCCTGG TGACAGGAGCAGGAGGGTTTCTTGGCCAGAGGATTGTCCGAATGTTGGTGCAGGAGGAAGAGTTGCAGGA GATCAGAGCCCTGTTCAGGACCTTCGGTCGAAAACATGAAGAGGAATTGTCCAAGCTGCAGACAAAGGCC AAGGTGAGAGTACTGAAGGGAGACATTCTGGATGCCCAATGCCTGAAGAGAGCCTGCCAGGGCATGTCTG CTGTCATCCACACCGCTGCTGCTATTGACCCCCTTGGTGCCGCTTCCAGACAGACCATCCTAGATGTCAA TCTGAAAGGTACTCAGCTCCTACTGGATGCTTGTGTGGAAGCCAGTGTGCCAACATTCATCTACAGCAGC TCAGTGCTTGTGGCTGGACCAAATTCCTACAAGGAGATCATCCTGAATGCCCATGAGGAAGAGCATCGTG AAAGCACATGGCCTAACCCATACCCATACAGCAAAAGGATGGCTGAGAAGGCAGTGCTGGCAACAAATGG GAGACTCCTGAAAAATGGTGGCACTTTGCATACTTGTGCCTTAAGACTCCCTTTCATCTATGGGGAAGAA TGCCAAGTCACTTCAACCACTGTGAAAACAGCACTGAAGAACAACAGCATAATTAAGAAAAATGCCACAT TCTCCATCGCCAACCCAGTGTATGTGGGCAATGCAGCCTGGGCTCACATTCTGGCTGCCAGGAGCCTACA GGACCCCAAGAAGTCCCCAAGCATCCAAGGACAGTTCTATTACATCTCTGATAACACCCCTCACCAAAGC TATGATGATTTAAATTACACCCTGAGCAAGGAGTGGGGCCTCTGCCTTGATTCTGGCTGGAGCCTTCCTC TGTCCCTGCTTTACTGGCTTGCCTTCCTGCTGGAAACTGTGAGCTTCCTGCTACGTCCAGTTTACAACTA TAGGCCACCCTTTAACCGCCTCTTGATCACAGTGCTAAATAGCGTGTTTACCATTTCCTATAAGAAAGCT CAGCGCGATCTAGGCTATCAGCCACTTGTCAGCTGGGAGGAAGCCAAGCAAAAAACCTCAGAGTGGATTG GAACACTAGTGAAGCAGCACAGGGAGACACTACACAAAAAGTCACAGTGATATGTGGAGGGCGAGGACAT GGCCCTGGGTGTTATCAGGTCCTCCAGAGAGGGACTTAGAAACAACTCAACTCTTATCAATTCCATTTTA TGCACTGTCCAACTTGTCTTTGGGTCACTAGCAACCTTGCAAGTCACTGGCCCAGTCACACACCATCAGT CCTAAGCACTTGCCCAGTAATGCACAGGCTGTTGTGACTCAGCTGCTGTGACCCAAGAATCTGTGGCTGG TACTGAATTGCCTGGAACCTCTTGTAGGTAGAATTTCCTCAGGACTTCCACATCTCCCTTCCATGTGCCA AAGCATTTCTTGTCTTGGAACATTTTGATACCTTTATGAAGCTCAAAGAACAATAAAATCTTTTAATGCT TAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4193717 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4193717 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11313 same embryo
	EMAGE:11315 same embryo
	EMAGE:11318 same embryo
	EMAGE:11317 same embryo
	EMAGE:11316 same embryo
	EurExpress:euxassay_006686 same experiment
	MGI:4825470 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source