EMAGE mouse gene expression spatial database

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EMAGE:11459

Gm996 predicted gene 996 ( MGI:2685842)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006922_01	euxassay_006922_02	euxassay_006922_03	euxassay_006922_04	euxassay_006922_05

euxassay_006922_06	euxassay_006922_07	euxassay_006922_08	euxassay_006922_09	euxassay_006922_10

euxassay_006922_11	euxassay_006922_12	euxassay_006922_13	euxassay_006922_14	euxassay_006922_15

euxassay_006922_16	euxassay_006922_17	euxassay_006922_18	euxassay_006922_19	euxassay_006922_20

euxassay_006922_21	euxassay_006922_22	euxassay_006922_23	euxassay_006922_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11459_wholemount_strong.wlz

11459_wholemount_moderate.wlz

11459_wholemount_weak.wlz

11459_wholemount_possible.wlz

11459_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11459_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9127
Entity Detected:	Gm996, predicted gene 996 ( MGI:2685842)
Sequence:	sense strand is shown >T9127 CAGCACCAGAAGCCTTGACCATGACCTGCAGCCAAGGCTGCACCATCCCCTATGCCCCAGGTAAGCCGGA CCAGCACGTGGGGTGGGGTGGGGTAAAGGGCTTGGGGTGCAGAGGAGGCTGCCCTGACCGGTATCTCCTC CCAGGGCAAAATCCCCGAAAGGCCGTGTGACTGGAGCTGCCAGCCTGGGGACGTCTTTTAAGATGACCCG CACGGACCCGCCGGACTTGCTGGTGTCGACCGTGTACCAGGACATCAAGGTGGTGGACCCAGGGCTCACG TCCAAACGCCAGCCATGTGAGCGATCCGTGGCCCGGCCTGCTGCGCCCACGCCTTTCAACAAGCGCCACT GCCGTAGTTTTGACTTCTTAGAGGCACTGGACGAGCCCACCATGGAGACACACCCAGAGCCGCCACCTCC TGAACCTGCGCCACCGCGCGCCCGACCCAGAGACAGCGAGCCTCGGCGCCGCACCCGTTCCAAGAGCGCG CCCCGTGCGTCCCAAGGCCTGGCGACTGCTCCAGCTTCGCCGCCCGTACTGCAACGCAGAGGTCGGGAAG CCCAGCGTGCAGTGCGGGTGGAGGGGTCGCCGCGCCGGGAGCCCTCGTATCCTGCGCTGAGAGCTCTCGC TAATGAGCTGCACCCCATCAAGCTCCAGCCACAGCGGGGAGGCCCCGGACGCATCGCGCCTCTATGCGCC ACCCCAGGCCGCTGCGCACCACCGGAACCACCCTCTGGACCTGTCCCCCATGTCCGCTGCCGTTTGGACA TCAAGCCGGACGAGGCAGTGCTGCAGCACGCCGCCCTGTCACGCACTCCTACCCCCAGTGACTTGTACTG TACTGACCCCAGAACTCTGTACTGTGACGGGCCACTGCCTGGGCCCCGGGACTACTTGGAGCACCGCAGT CAACCCTTCACAACGCCTCCAGGTCCCACCCAATTCTTCTACACCGAGGAGCCAGAGGGCTACGCAGGCA GCTTTACCACAAGCCCCGGCCTTCCCTTTGATGGCTACTGTTCCAGGCCCTACTTGTCCGAAGAACCCCC TAGACCCAGTCCAAGGCGTGGGGGCAGCTACTATGCTGGCGAAGTACGCACCTTCCCGATCCAGGAGCCC CCCTCCCGCTCCTACTACGGGGAGACAACTCGAGCCTACGGTATGCCTTTCGTTCCCCGGTATGTCCCAG AAGAGCCCCGGGCGCACCCTGGAGCCCGCACTTTCTACACCGAGGACTTTGGGAGGTACCGAGAACGCGA TGTACTGGCTCGGACTTACCCGCACCCGCGCAGCAGCCCACCCTGGGCTGACTGGGGTCCGAGGCCTTAC CGCACCCTTCAGGTAATGCCGCCTCCCGCCCCTGGACCACTGTTGGCCTCGTGGCACGGCGGCACCGGCA CAAGCCCACCCCGGCTGGCCACCGATAGCCGCCACTACTCTCGATCCTGGGACAACATCCTAGCTCCTGG TCCTCGTCGTGAAGATCCTCTAGGACGCGGCCGCAGCTATGAGAACCTCCTGGGACGCGAGGTTCGGGAT ACGAGGGGTTCATCCCCTGAAGGTCGGCGTCCACCAGTCGTAGTGAATTTGTCCACCTCACCCAGACGCT ATGCAGCACTGTCGCTGTCCGAGACGTCGCTGACAGAGAAGGGCCGCGCGGGAGAGAGCCTGGGCCGCAA CTGGTACGTCACGCCAGAGATTACCATCACAGACAACGACCTGCGCTCAGTGGATCGCCCGACTGCCAAG GGCTGGGAATTGCCTGGAGGCCGACCACGGCAGCCCGTATCCACGGTCCCTGAGGGTCCTGCTTCAAGCC GCCAGCGCAGCCTCGAGCAGCTGGACGAGCTCATCACTGACCTGGTCATCGACTCACGGTCGCCAGCCCA GGCCCCGGAGCCTGCCGCCGAAGGTTTAGGCCGCCAACTACGCCGCCTGCTGGACTCACGGGCTGCAGGC CCTGGAGGGGCCACCCTGCTGGCGCCATCGCGCTCGCCCCCGGCCTCGGCTGGCAGCACTGAAGAGCCCA CCGGCTCGGGGGAGGCAGCGGACGCCTCCCCCGAGCCCAGCGCAGACGAGGATGACTTGATGACCTGCTC CAACGCGCGCTGCCGGCGGACAGAGACCATGTTCAACGCCTGCCTCTACTTCAAGTCGTGCCACAGCTGC TATACCTACTACTGCTCGCGTCTGTGCCGCCGAGAGGACTGGGACGCGCACAAGGCGCGTTGCGTGTACG GCCGCGTGGGCAGCGTGTGCCGCCACGTGCTGCAGTTCTGCCGCGACAGCAGCCCAGTGCATCGCGCCTT CTCTCGCATAGCGCGCGTCGGATTCCTGTCGCGAGGCCGTGGCGTGCTGTTCTTGGGCTTCCCGAGCCCA GGCTCTGCCGACAACTTCCTACGCTTTGGCCTCGAAGGGTTGCTGTTATCGCCCACCTACCTGTCGCTGC GTGAGCTGGCTACACATGCGGCGCCCCTGGGTAGCTATGCACGAGAGCTGGCGGCAGCAGGAAGGCTCTA TGAGCCGGCAGAGTGCTTCCTGCTTAGCGTGTCCGTGGCTGTGGGCCCCAGCGCTGCACCCCCCGGAGCC GCTGCCCGGCCCGCGCCGCGCACTCCTGGGCCCACGGTGCGCAAGTTCGCCAAGGTGGCTCTGGCCGCTG GCAGCCCGACGCGTCCTCCTCCTGCGCGTGGCGGAGAGCCAGATATGGAAACCTTGATCCTGACGCCACC CCCAGGTACTGCGGGCCTGGACGAAGAAGGTGAGGCCGGCCGGCGCGCACGTGAAGTAGCCTTCATCCAT ATCCAACGTGAGCTTCGAATGCGCGGCGTCTTCCTCCGCCACGAATTCCCACGGGTCTATGAGCAGCTCT GTGAGTTTGTGGAAGCTAACCGGCGCTTCACGCCCACAACCATCTATCCCACGGATCGGCGCACTGGCCG CCCCTTCATGTGCATGATCATGGCTGCATCGGAGCCGCGTGCACTAGATTGGGTAGCCAGCGCCAACCTG CTGGACGACATCATGTGAACCATGGAACTATTAGGCCCAGGCCCCGACCCCGAGCCCTGCCTACTCTACT CAGGCCCTGCCTGATCCATCACGAAGGCCCCCAGAGTCTGGACCAGTTGGTTCAGCGCCTTCCAAATCCC CTGGGCCTCCTTTAGTCTTATACAAATTCTCTGCCCTGAGCTCCACCCACTCCACACCTCTAATTTTGCC TGGGAGCCCCCACCCCCACCCCTGTCCCGTTTCCAACCAGCTGCTTCTTTTCTTCTCTGTTCGTAACCGG GCCATCCAAATTCCACATCCTGGGCTCTCCCCAAGCCAGTCAAGGAGAGGGGGCTCCCCAAGGGCCCGGT CCTCGCCCTCGGTGCTCCCCACTGGGAGGTGGGGTGGGACTGAGGACCTCTGGGCTCTGCCTGCTACACT CATCCCATGGGGACAAACTCAGTTGGTGTATGTTGTGGGTGCTTGCTATAGCTCTCCAACCCTTCTAGTC CAGAAATCCATGTTAACCAAAGCCCGATGTCCTTACTTCGAGCACTTCCCCTAACCCTCCTGTCTGGGGT GTATCAGGCTCAGAGGGCTACTCCCTGTGCACCCCTATGAGGTTGAGGCAATGGGCGTAGCCAGCACTGT ACACGTACCAGTGCCGCCTGTCCTTCAATACTCTCACCTTAGTCTGGGCACTTGTAGACAAGTTGGAATA CTGGGGAATTTTCAGGTTAAGGCCAAGGTACAATCCACCCTGCCAGATGGAAGCCTGAGCAGGGGAAGCA GTGTGGGCGGCGACCCAACCAGAGTTAAGATCTGGGTAAGGATTTGAGCTCCCAACACCTACCCAACAAG ATGGCCTTAAAAATCTGCCTTCCAGAGAAGATGGAGAGGTGGGAAGCGTGTGCTGACGAATTAGCAGACA ACCCTACTGAGAGCAGCTCCTACCACGGAATGACTTGGGAATGAGTAAGGAACAGGGCTGTCCCTAATTA GGAACTGGACTAGGAAAGGTGCTTAGTAGTGAGCCACCAAGTGTGGGTACGGTTAAGGAGTCGGTGCCGA GGCCTGCCCCGAATCCAATCCTGTGTGCAAAAGTGCCCTGGCTGCACTCCAAGCTGAGAGAGAAGCCAAA GCCTGACGTGTTGTGGAGAGGTCTAGGCTTCCCTAAGCCCCTTGCCTGACCCCGTACCTGGATCTGAGCC CAGGACTTTGTGGAGGGTGTGACCCAGAGAGGGCTTGCGACCAGATTCTCCAAGAATCTTGGGAGAAGAA GAAGCCCCAAGCCCTTAGGAGCTCTGAAAGTTCAATTGCAAGAACCTGGGACACACACCTATGCACGAGG GTGTGCACACGCGCCAGCTACCCTCGACTGGATGTCCCCGGGACACTCCAGCTCTCTCTCCTTGGCCCCT GTCTTAGCACATGCCCGGATGCCGCTCTTGCAACACTTGCTGCCCCGCCGCCTGCCGCCCTCCCCAGGGG CTCCACAGTCCCTCCTGCGGCAGACTGGCAAGAACGCCTCTGACGAGAAGGGCAAGTGCTTTCGAACCAA GTAAAATAGAACTTGAATGTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6825178 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6825178 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11461 same embryo
	EMAGE:11462 same embryo
	EMAGE:11460 same embryo
	EurExpress:euxassay_006922 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source