EMAGE mouse gene expression spatial database

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EMAGE:11461

Uba6 ubiquitin-like modifier activating enzyme 6 ( MGI:1913894)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006898. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006898_01	euxassay_006898_02	euxassay_006898_03	euxassay_006898_04

euxassay_006898_05	euxassay_006898_06	euxassay_006898_07	euxassay_006898_08	euxassay_006898_09

euxassay_006898_10	euxassay_006898_11	euxassay_006898_12	euxassay_006898_13	euxassay_006898_14

euxassay_006898_15	euxassay_006898_16	euxassay_006898_17	euxassay_006898_18	euxassay_006898_19

euxassay_006898_20	euxassay_006898_21	euxassay_006898_22	euxassay_006898_23	euxassay_006898_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11461_wholemount_strong.wlz

11461_wholemount_moderate.wlz

11461_wholemount_weak.wlz

11461_wholemount_possible.wlz

11461_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11461_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9129
Entity Detected:	Uba6, ubiquitin-like modifier activating enzyme 6 ( MGI:1913894)
Sequence:	sense strand is shown >T9129 AGGCGATGGAGCGGTCCGAGCCGTTGGCTGTGCTTTCCTGTGAAGAGGCGTCCTGCTCCTCCTGGGGAGC CTGCGGTGCAAGTAAAAATTTACCTACCATGACAACAGAATCTTTGGAAATCGATGATGGATTATACAGT CGCCAGAGATATGTTCTTGGTGACACAGCAATGCAGAAGATGGCCAAGTCCTGTGTCTTCTTAAGTGGTA TGGGTGGTCTTGGAGTGGAAATTGCAAAGAATCTTGTTCTTGCAGGGATTAAGGCCCTAACAATTCATGA TACAAAAAAATGCCAAGCATGGGATCTAGGGACCAATTTCTTCCTGTGTGAAGATGATGTTGTTAATGAG AGAAACAGGGCTGAAGCTGTACTTCATCGTATTGCAGAACTAAATCCATATGTTCAGGTCTCATCATCCT CTGCCCCTCTTGATGAAACCACAGACCTCTCTTTCTTAGAAAAATATCAGTGTGTAGTATTGACTGAAAT AAAACTGACATTACAAAAGAAGATCAACAATTTTTGCCATTCTCATTGCCCTCCAATTAAGTTCATTAGT GCAGATGTACATGGAATTTGGTCCAGGTTGTTTTGTGATTTTGGTGATGAATTTGAAGTTTCAGATACAA CAGGAGAAGAACCAAAAGAAATTTTCATTTCAAACATAACGCAAGCTAATCCAGGTATTGTCACTTGCCT TGAAAGTCATCCTCACAAGCTTGAGACAGGACAGTTCCTAACATTTCGAGAAATTCATGGAATGACAGGC TTAAATGGATCTGTACAACAGATAACTGTCATATCACCATTTTCCTTCAGCATTGGTGATACTACAAAAC TGGACCCATATTTACATGGAGGCATAGCCGTGCAGGTGAAGACTCCTAAAACATTCTGCTTTGAACCCCT GGAGAGCCAGATAAAACATCCAAGGTGCCTTATTGCAGATTTTAGCAAACCTGAGGCACCTTTAGAGATT CATCTCGCTATGCTTGCCTTGGACCAGTTTCAGGAGAACTATAACCGTAAGCCAAATATCAGATGTCAGC AGGATTCAGATGAACTATTGAAGCTAACAGTATCTATAAATGAAACCTTGGAAGAGAAGCCTGAAGTGAA TGCTGACATTGTGCACTGGCTTTCGTGGACTGCCCAAGGCTTTTTACCACCACTCGCTGCTGCAGTTGGA GGTGTTGCTAGCCAAGAAGTCTTAAAAGCTGTGACGGGGAAGTTCTCGCCCTTGTGTCAATGGTTGTACC TTGAAGCAGCAGATACTGTGGAATCCCTAGGCAATCCTGGGCATGAAGAGTTTCTCCCACGAGGAGATAG ATATGATGCCATACGTGCCTGCATTGGAAATACATTGTGTCAGAAGCTACAAAATTTGAATATCTTCTTA GTAGGATGTGGTGCCATAGGCTGTGAAATGTTAAAAAATTTTGCATTACTGGGTGTTGGTACAGGCAGAG AGAAAGGAATGGTAACAGTTACAGATCCTGACTTGATAGAAAAATCCAATTTAAACAGACAGTTCCTGTT TCGTCCTCATCACATACAGAAACCTAAAAGCTATACTGCTGCTGAAGCAACTCTGAAAATAAATCCTCAA TTAAAAATAGATGCACACCTGAACAAAGTTTGTCCAGCCACTGAGAGTATATACAGTGATGAATTTTACA CTAAGCAAGATATCATTATTACAGCACTAGATAATGTGGAAGCCAGGAGATATGTAGACAGTCGCTGTTT AGCAAATCTACGGCCTCTCTTAGATTCTGGAACAATGGGCACCAAGGGACATACTGAAATTATCGTACCT CAATTAACTGAATCTTATAATAGTCATCGAGACCCCCCCGAAGAGGAAATACCATTTTGCACTCTAAAGT CCTTTCCAGCAGCTATCGAACATACTATCCAGTGGGCAAGAGACAAGTTTGAAAGTTCCTTCTCCCACAA ACCTTCACTGTTTAACAAGTTTTGGCAAGCATATCCCTCTGCAGAAGATGTCTTACAGAAGATACAAAAT GGACAAAGTTTAGAAGGCTGTTTTCAAGTTATTAAGCTACTTAGCAGAAGACCTAGAATTTGGTCCCAGT GTGTAGAGTTAGCAAGACTAAAGTTTGAAAAATACTTTAACCATAAGGCTCTGCAGCTTCTTCACTGTTT TCCTCTTGAGACAAGACTAAAAGATGGCAGTTTGTTTTGGCAATCACCAAAAAGGCCGCCTTCTCCAATA AAATTTGATTTAAATGAACCTTTGCACCTCAGTTTTCTTCAGAGTGCTGCAAAACTATATGCTACAGTAT ATTGTATTCCGTTTTCAGAAAAGGACTTATCAGTGAATAGCCTCATGGACATTCTTTCAGAAGTTAAAAT TGAGGAATTCAAGCCTTCCAACAAGGTTGTTCAAACAGACGAAACTGCAAGGAAGCCCGACCATGTTCCT GTCTCCAGTGAAGATGAAAGGAATGCTGTTTTCCAACTAGAAGAGGCTCTTTCCTCTAACAAAGCTACCA AAAGTGACCTTCAGATGACAGTGCTTTCATTTGAAAAAGATGATGACCGTAATGGACACATAGATTTCAT CACAGCTGCTTCCAACCTTCGAGCCAAAATGTATAGCATTGAACCAGCTGACCGTTTTAAAACTAAACGC ATAGCTGGTAAAATTATACCTGCTATAGCAACATCTACTGCTGCAGTTTCTGGCTTGGTTGCTTTGGAGA TGATCAAGGTAGCTGGTGGCTATCCCTTCGACGCTTACAAAAACTGTTTCCTTAATTTAGCCATTCCAAT AATAGTGTTTACAGAGACATCTGAAGTAAGAAAAACTGAAATCAGAAATGGAATATCATTTACAATTTGG GACAGATGGACTGTACATGGAAAAGAAGATTTCACCCTCTCAGATTTCATAAATGCTGTCAAAGAAAACT ATGGAATTGAGCCGACAATGGTGGTGCAGGGAGTCAAAATGCTTTATGTCCCTGTAATGCCAGGTCATGC AAAAAGGCTAAAGTTAACAATGCACAAACTTGTGAAGCCTTCCACTGAGAAGAAATATGTGGATCTTACT GTGTCATTTGCCCCAGACGCTGATGGAGATGAAGATTTGCCCGGCCCTCCAGTGAGATACTACTTCAGTC ATGACACTAATGAGTAGCATTGTTACCGAGATACTCCAGCACTACTTTATTTTGGAAAGTGTACTTAATT CAGAAGCTCAGCTCATAATACTGTGGATTTCTTTTTGCCTTAATGTAAGGGAACCATCAGTAGGGAACTT ACTAGAGTGGAGAATTACAAACCATTTTGAAGCATAGTACACATTTGTCTGTTGCTCCCTGCGGTTATGA TCACAGCACCTTTGAACTGGAATACCATTTTCTAATACTTATTAAGCCCTGTGGATGAAAGAAGGAACAA GAAATCATTTATCTATGACCAGAAGAAATAAAGATGAAGAAATTGAAAATAGTGAATTTGTACTATTCAG AAAGTTAAATCCTTCTTCAACGTTTTCTTTTTTATTTGGTATTTGAGACCCATTATTTCAATATAAATAG TGTGGCACTGTGCTGCTTACATGATTAGGCTTTGAAAATTCCATAGCTGATAGGAAGAGGGGTGGCAGAT AGGTGATGAGTAGAGATTTAAAACTGTAGTTACAGTTAGTGTCCTGCTACCTGTGCTAACAGTGTGATAT ATTACTCCCTTCCCTAAGTCAGGATTGTCCCTGATCTGATCAGTGTTGTAAACAGTGAGGTAACATGACA TGTTAACTTGGACTAAATGTTAACAGTATTACATGCACTCTGATAGAGAATCCATAAATGTGAGCAGATA AATATGGTTAATCATTTGATTGTTAATATACCTTTATTTTATTTATCTGGAAGTCTAAACTAGTCATAGT ATTTAAGACCAAAAATTTTTTATAAAGGGAATATTCTTAGAAATAGATGAAAGTTACAAATTAATTTGGT TGTCTTTTTTTCCCTTTCCAGCAATGTAATGGAGAAAAAAAAAAAATATATATATATAGTATTTTTTTTT AAAGGAGGCATGTTATTTCACTCTTCATTCCCAAATTGGGGATTGACTGAAATAGAAAAAATTATATTTT TTTAGATTTTTCTTTACTGAAAGAAATACTCCATTGCCTGATGTGAACAGTTGCTACTTATGATATAATT GGGTCCATTAAAATATATAAACAATACTTGACTGTAAAATCACTGAAAACAGCCAGTAAGTCTTTTTTAC AAGGGTTATCACGCATTCTAAACAAGTGCTTACAGCCTCTGGAAGTTACTTGCCTGGAGTTCCTATTCTT AAGACCATTACTAAATACTTTGATGAATTAAAATTTAGATTGCATCAGTATTAACTCCAATGGTATCTCT TTATACTCTATACTTTCTTTCCATTTACAAACAATTTATGATCTCTGTGGTAACCAGCTTTTTGTTTCCT CCTTTTACGAAGTGTCCAGTTGGGAAATGGGGTGAGTTTGTACAATAAAATGATCTGTTCATTCAGAGGT GGAGAAAAGTGGACAGGGTTTGTTTTAAAGAACTGGCTACCTTTAAAATAAACTATAGTTTTTATCATAT CCTACCTTTCCCATTGTTCATAAATTGAATCCATGAATATCAAAGGCTCTGTGGCTTAGAACTTAATGAC AAAAAGAAGTCCTATTTTCCAGTTTTCACTTTGTTTTCTCTTCTTTGGTAGTAACGTATTCTGTAGGTAA TCATTCAGGAATCCTCAAACATGAAGCAATAGCTTGGCTCTGTTTACTTAAGTCTACACGTATCTTTGAA AGTAAATGAGAGCCATGCTTTGTAGAGTGCTTTAGTAAATAAAATCAACCAGGTTTGGTTTGTTTCTAAT TAAATCCACAACCATTTTGTCATTGGGTTTTGTTTTTGTTTTTCTGTAGGTAGTGATTATTACTGTCCTA CTAAATTATACTGAACTGGAAAGCCATAATGAAGTTAATGACTTTTTTTTTTTGTTCATTTTGTACTTTA AGATTCTTGGTACTAAAATATAGCTATTGAATACTGAGTGCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6825308 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6825308 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11459 same embryo
	EMAGE:11462 same embryo
	EMAGE:11460 same embryo
	EurExpress:euxassay_006898 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source