EMAGE mouse gene expression spatial database

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EMAGE:11468

Isca1 iron-sulfur cluster assembly 1 homolog (S. cerevisiae) ( MGI:1916296)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006863. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006863_01	euxassay_006863_02	euxassay_006863_03	euxassay_006863_04

euxassay_006863_05	euxassay_006863_06	euxassay_006863_07	euxassay_006863_08	euxassay_006863_09

euxassay_006863_10	euxassay_006863_11	euxassay_006863_12	euxassay_006863_13	euxassay_006863_14

euxassay_006863_15	euxassay_006863_16	euxassay_006863_17	euxassay_006863_18	euxassay_006863_19

euxassay_006863_20	euxassay_006863_21	euxassay_006863_22	euxassay_006863_23	euxassay_006863_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11468_wholemount_strong.wlz

11468_wholemount_moderate.wlz

11468_wholemount_weak.wlz

11468_wholemount_possible.wlz

11468_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11468_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 18 19 20 moderate expression: see section 12 16 17

4th ventricle

strong

regional

strong expression: see section 05 06 07 08 11 18 19 20 21 22 moderate expression: see section 03 04 12 13 14 17

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11

liver right lobe

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22 23 24 not examined expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9197
Entity Detected:	Isca1, iron-sulfur cluster assembly 1 homolog (S. cerevisiae) ( MGI:1916296)
Sequence:	sense strand is shown >T9197 CCCGCCGTGTCCATGGAGAGAAGTTGAGTCCCCACGCTCCAGCCGGAGGCTGCGGGTGCCGCGACGTGGA CATGTCGGCGTCGTTGGTCCGCGCCACCGTGCGGGCTGTGAGCAAGAGAAAACTGCAGCCCACGCGGGCG GCCCTCACACTGACCCCCTCTGCTGTAAACAAGATAAAACAACTTCTTAAAGACAAACCTGAGCATGTGG GTCTGAAAGTTGGCGTGCGAACCAGGGGCTGTAATGGCCTCTCTTACAGCCTGGAGTACACAAAGACAAA AGGAGATTCTGATGAAGAAGTTATTCAAGATGGAGTCCGAGTGTTCATCGAGAAGAAAGCACAGCTAACC CTGTTAGGAACAGAGATGGACTATGTGGAAGACAAACTGTCCAGTGAGTTTGTGTTCAATAACCCCAACA TCAAGGGAACCTGTGGCTGCGGTGAGAGCTTTCACGTGTGAAACCTCAGGACTGTGAGCTCCAGGTGAGC CGGGTCTGCCTCGGCGCTTCCTGAAGAAATCATGTGATGGCACGTGCTCAGGCGTGTGGCTGCCTGCCTC GTGGGTGAAAATAAAGTGACGCATTTGAAAACCAAGCCAATGTGGGAAGAGTTCCAAAAGCAATGTCTGT ACTCTCTGTAAGACACAAACTGAGAAAACATGGCGTCTATACTCTCAAAGACACAAACTGAGAAAACATG GCGTCTATACTAAGACACAACTGAGAAGCCGTAGATTTCCTTGAGTCATTCTGTTCTCTACAGGAAACTC CTACCCTGCCCTTGTGCTGTAGCCTGCTTTGTGCTAGAACCAGCTTCATGGCCATTGCTTTGCTGGGAAT TGAGGAATGGGATAACAGGCGTGACCTACATCACAGCATGGCTTGAGTGTGTCTATCCCTTTCTCAATCT TTGCCAACCCCCGATCTCAGGCAGGACAACTGTGGGTCACGGTGTAGTGGGACCTCATTTCCCTCACCTT TGGCATGGCCCATTACATTTTTACCAAAAATGTGATGATCACATGCCTCGTTTGCCAACCCTTCAACAGT TCCTGAGTTCTGAGTTCCAGTATCTGAACTTGGGCACCACCAGGCCTGGGTTAGCACATCTCGGTGAGCT TCATGATGGTTTGCTGTAGACCTTTCAGTTAGAAACGCTTGTGCTAAGCAGCCAGCTTGCTTGCTCAGTT CCTGCCCAGCTCTCCCTTCCTCGAGGTCACAGCAGTGCTGTCTGAGACATTCGGTGAGGAGAGTGGCGAT GGCTTCCCGCCTGGCCAGCGCTCTCACCAGAGTTTCTTAGTTTATAGAGACTTGTTAGTTTTCTTTTGAC TAGACTCTTGTGTAAACCCTTTCGTGTGAGTTGCCATCTTGTTCGTTTAGAACTTTGAAACAGTAGTTAC AGACATCTGCAGCCCGGGATTCCTCTCAGCACCGGGCCTGTGGAGCGGAACATGCCATTCTGACACTTGC CAGTATGCATATGCTTTCTGGTTCCTAAACGAAATTGCTAAATATTTTTAAATATTTTTATCACCAGTTT TTTGAGCCATGGTTTTGCTGTATACTACAGGCCAGCCTTGAACGCACAATCCTCCTGCTTCAACCTTCTG AGGCATGTGCTACCATGCCTGGATCCCAAGTTTTTCCTTAAGTGGTCTTGATTGACTTGGGTTGGACATC TTAAGTAACCTGTGAATTCTTTTGTGGAAGGCTGAGTCTCATGCAGCTGAGTTTAATATTTGTGCTATTT ACTAAAATACCTACCACCAAAATATACCAACTCGTAGTTTTATATGAATGTTGATGAGTCTGTATCATAA ATAGAATTGTTGATACATCCTTAATTTGTGCAATATTGTATGAAGAGATTGAGTTGTCGATTAAAAACAT GCATCTTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6830054 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6830054 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11471 same embryo
	EMAGE:11470 same embryo
	EMAGE:11469 same embryo
	EMAGE:11473 same embryo
	EMAGE:11472 same embryo
	EurExpress:euxassay_006863 same experiment
	MGI:4825627 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source