EMAGE mouse gene expression spatial database

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EMAGE:11471

Ubtd2 ubiquitin domain containing 2 ( MGI:2444752)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006836. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006836_01	euxassay_006836_02	euxassay_006836_03	euxassay_006836_04

euxassay_006836_05	euxassay_006836_06	euxassay_006836_07	euxassay_006836_08	euxassay_006836_09

euxassay_006836_10	euxassay_006836_11	euxassay_006836_12	euxassay_006836_13	euxassay_006836_14

euxassay_006836_15	euxassay_006836_16	euxassay_006836_17	euxassay_006836_18	euxassay_006836_19

euxassay_006836_20	euxassay_006836_21	euxassay_006836_22	euxassay_006836_23	euxassay_006836_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11471_wholemount_strong.wlz

11471_wholemount_moderate.wlz

11471_wholemount_weak.wlz

11471_wholemount_possible.wlz

11471_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11471_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9137
Entity Detected:	Ubtd2, ubiquitin domain containing 2 ( MGI:2444752)
Sequence:	sense strand is shown >T9137 CGGAGGCTGAGCGGCGAGAGAGCGGCGCCTGGCCCGAGGCGGCGACGCGGGAGCAGGCTGAGGCGGCGGC CGGGACGAGCGCCCTGAGGTGGCGGCCGCGGCCCCATGGGCGGGTGCGTGGGCGCCCAGCACGACTCTTC GGGCAGCCTCAACGAGAACTCGGACGGCACGGGAGTTGCTCTAGGTCGTAACCAGCCTTTGAAAAAGGAG AAACCAAAATGGAAAAGTGACTATCCCATGACAGATGGACAACTGCGCAGCAAGAGGGATGAATTCTGGG ACACTGCACCAGCTTTTGAAGGCCGGAAAGAGATCTGGGATGCCTTGAAGGCCGCTGCACATGCTTTTGA AAGCAATGATCATGAACTGGCACAGGCAATCATTGACGGTGCAAACATAACACTACCACACGGTGCACTG ACAGAGTGCTACGATGAGCTGGGGAACCGGTACCAGCTGCCAGTCTATTGCCTGGCACCACCAATCAACA TGATAGAGGAAAAGAGTGACATAGAGACTCTTGATATTCCTGAGCCACCTCCCAATTCTGGACACGAATC TCAGCTCCGCCTGCGGCTCTCCACAGGCAAAGACCTCAGGCTCGTGGTCCGCAGCACAGACACAGTGTTT CACATGAAGAGGCGCTTACACGCCACAGAGGGCGTGGAGCCAGGCAGCCAGCGCTGGTTCTTCTCTGGCA GGCCTCTCACGGATAAGATGAAGCTGGAGGAGCTGAAGATCCCCAAGGACTATGTGGTGCAGGTTATAGT GAGCCAGCCTGTGCAAACCCCAACACCAGTGGAGAACTGAGCTGGACTTGCCTTCTTCCAAGATCCCGTG GCTCCTTTTTATTGTTACCATTTCTTACTCTTAAGGCGTGAAATTTATTTTCACTGCTCTGAATTCCCTA TGAATGGATTTAGTTCTGAGGAATTACCAGTGAAAAATTCCATCTGTGATGGAGACCAACAAAAATAATA AAACACACAGAGCCAGCCTCTGATACTCTTATAATTACAATTTCTAATTTGAAAGACAGTTAAGAAATAG ATAACCATTTATTTTAAAACATTTATCAACTGTGTAATGGCTGTATTTAAATGATCTCGAATGTAAACAG ACACCAGACCCATCAAAAACAGCACCAAGCACCTTTTGGACACAGAATTTTTATGACATACATGTCAAAT TTTTTTTTTTCCAGAACCATCAATAACAGTTACAAAACCTAATCTTTTTTTTCTTTTAAAGTGGAACCTA AAAGAGCATAGAGTTTTGCTTCTCTTCTGGGTTTGTAGTCCTACCCACCAGGAGGCAGTGAGACTGGGAG TTGTCAGTGTTCCTTCCCCTCCCACCAAGTTCTGCTCCCTGTTGACATGCACTCAGTCCTGTGGCCGTCA TGCTGGCAGCGGGCACCCTCTGGCGCCCTGAGCAGCACGTTAGTAAGTAGTTAGAGCTTGTATTCTCAGT TGAGAAGTTTCTAGTCTTGCTTCATAGTAATCTTAACATATCTGTGGACCCAGGCTTGACAGCTAGCTAC TATGTGATGTCTTTGCTGACCCTGATGCTCTGCGTGACCATTTCTGTAGCTTCCTGGCCCCGCCTATATA TTCCTGGGCCAGGCAAGACAGAAATGGAGTATAGCTGCTTCCAGTCCAGAATGAAAAGCAATCTTCTCCT TCCTGCCTGCAGGGAATGTGAGTAAAACCCTCTCTGCCTGTTCCTGGGAAAACTGTGCCTATTCTAAGCC TTGGAGAAAATTCCAATTCAAAATGTTCGTGTGTGATAGCTAACTAGTGTCATTTAAACTGCATTGGTGG GAACAGATGGTAGCGCCTGGCTCAGACTGGAGTGCTTTCACTTAACTCTGACTCCAGTCACTGTGGTGGC AGGGTTCCTCCCACACCTTCCCAACAAAGGTGAATTAACCTCCATCTGCACACTGGGCTAAGGGACTGAA CATAGTAAATCAGGCTCACCTTCTCTGGAGAAAGCTGCCAAGGGTTATATTTAATCTGGAGCCTTTTAAA AGCTGTTTTTGCCAGGCATGTTGTACCTGCCTTTAATCCTGGGCCTTGAGAGGTAGAAAGAGGCAGATCT CAGAATTTGAGACCAGCCTGGAATACACAGCTAGTTCTAGGCCAGCCAGAGCTACTTAGTGAGACTGTGT CTTAAAAAAAAAAGTTGGTTCCCTGAGAAGGGGACTATCATGTGACCACACTGTCTCTGAAAGTTTGTGA GATGTGAACAGTAGATGAAAGAAGGGTCTGTGCTTTTCTCAGTCAGGAAATACCAAACCTCCTATTCGGA TATAAAATGCCATTCATAATTTTAGTGGCAGAATGAAACCTCTAAGCAGTTTAAAATGTAATTTTTAAGT ATTAAATTTCTTATAGCCTGTTTAAATATATCTATAATTATAACTTACATTTCTGAGAACTTTAACCATT TCCCTAATTCTGTTCATCATTTAGTTTGTGATTGTATCTAATTTAGATTTGGTGGTGACTGATCTAAATT CAGAAGTGCACACACAGCTTTAGCAACCAAGGATGCCCACATTTCCATGTGGAGGCTCTAGGGTATTCCA GTGCATCTCCCTCCCACCATCAAGAAGGTTGGTTCATTTGTCATATTGAATAACAGTATCTCAAAGTCCG TCAGTTGGAAAAGTGGTTAAACCTATTATTTTTCTAAAGGAAATTTGATGGTGTCATGAATAACCTGATT GTTAAAAGGTCTTGTTTCTTGAGGGTTTGTCCTAAGAGATTTGTGGGGTCTTTTGGTTTTTGTTTGTTTG TTTGTTTGTTTAAATTTTAAAGGAACATATACTTTGAATAACAATCCTCCAGGCATGGTGAGAAAAAAAC CTAGGTGCTGGAAATTGTCAATAGGTTAATAACCTAGGTGAGAAAAAAACATGGTGCTGGTAATTGTCAA TAGGTCGATGTAAGATTGGATCAATACTTGATGTGTATCATTTTAGTATGTAGGGGTTTTGTGCTTTTTT TAAAAAAAAAAATTCAACTTTTAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6825513 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6825513 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11470 same embryo
	EMAGE:11468 same embryo
	EMAGE:11469 same embryo
	EMAGE:11473 same embryo
	EMAGE:11472 same embryo
	EurExpress:euxassay_006836 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source