EMAGE mouse gene expression spatial database

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EMAGE:11472

Zfp623 zinc finger protein 623 ( MGI:1926084)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006895. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006895_01	euxassay_006895_02	euxassay_006895_03	euxassay_006895_04

euxassay_006895_05	euxassay_006895_06	euxassay_006895_07	euxassay_006895_08	euxassay_006895_09

euxassay_006895_10	euxassay_006895_11	euxassay_006895_12	euxassay_006895_13	euxassay_006895_14

euxassay_006895_15	euxassay_006895_16	euxassay_006895_17	euxassay_006895_18	euxassay_006895_19

euxassay_006895_20	euxassay_006895_21	euxassay_006895_22	euxassay_006895_23	euxassay_006895_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11472_wholemount_strong.wlz

11472_wholemount_moderate.wlz

11472_wholemount_weak.wlz

11472_wholemount_possible.wlz

11472_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11472_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9217
Entity Detected:	Zfp623, zinc finger protein 623 ( MGI:1926084)
Sequence:	sense strand is shown >T9217 GGCGTGCGGCAGCGGTGCGGGCTTTGTGGCCGCCCGGGGTTACCCGCCTGGATGCTGCTCGGCTTGGTTC CGCGCCGTCCTCTCGCCGCCGAGCTCCGGATTCTCCTGTAGGGAGCCAGTGGCAAGAAGGGGATCCGGAA GCCTGAATTTCTGGGGATGAAAATTCACATAAGATGATGTCAGACGAACTCACAGTGCTGGAGCCCTCTG CCTCTGAGTCTCGGGAATTTCATGAAGACAGATTGGCGCAGCTGTTGGGGAACCCAGACAAGCAGAGCTT GGAGAGTCCCTCCTCTCAGGACGGGGGCTTCACTCAGATGACAGTGACCCACTGGAAGATTCAAACAGGA GACACAGCCCAAACGTGCAGTAAGTCAGGAAGAAACCCCATTCTCAATTCAAACCTTCTTATACTTCAGA GAGAACTCATAGATGCGGAGGCCCGTTCCTGTGGTGGTGGTGGCAAAGGCTTCCCATTTAATTCAGATGT AGTTCCGCATCAGATCTCGCACACGGGGGAGAAGCCTTACAAATGTGATCATTGTGGGAAAGGCTTCAGT CAGACCTCCCTCCTCACTGAACACCAGAGAGTCCACACTGGGGACAGACTCTGTGTGTGCCACGTGTGCG GGAAAGACTTCGTTCACTACGCAAGCCTGAGGGAGCATCAGCATGTCCATACTGGGGAAAAGCCGTTCAA GTGTGCACAGTGTGGAAAAGCATTTTGTCACAGTTCAGACCTGCTTCGACACCAGAGGGTTCATACCAGG GAGAGGCCTTTTGAATGCAAAGAGTGTGGGAAGGGTTTCAGTCAGAGCTCCTTACTCATTCGCCACCAAA GAATTCACACAGGAGAAAGGCCCTATGAGTGTAACGAGTGTGGGAAATCCTTCATTCGGAGTTCAAGCCT TATCCGCCATTACCAGATCCACACAGAAGTTAAACAGTATGAGTGCAGAGACTGCGGCAAAGCCTTCCGA CACCGCTCGGACCTCATCGAGCACCAGAGGATTCACACTGGAGAGCGGCCCTTCGAGTGTCAGGAGTGTG GGAAGGCCTTTATTCGGAGCTCGAAACTCATTCAGCACCAGCGGATTCACACGGGAGAGCGGCCTTACGT GTGCAACGAGTGTGGGAAGCGTTTCAGTCAGACATCCAACTTCACCCAGCACCAGCGAATCCACAGTGGA GAAAAACTCTATGAGTGTAACGAATGTGGGAAGGCGTTCTTTCTGAGCTCATACCTCATACGACACCAGA AGATCCACACTGGTGAACGGGTGTATGAGTGTAAGGAGTGTGGGAAGGCTTTCCTGCAGAAGGCCCACCT CACTGAGCATCAGAAGATCCACACTGGAGACAGACCCTTTGAGTGCAAAGACTGTGGGAAAGCCTTCATC CAGAGTTCTAAGCTATTGCTGCATCAGATTGTTCATACCGGGGAGAAGCCCTATGTGTGCAGTTACTGCG GGAAAGGCTTTATTCAGAGATCCAACTTCCTTCAGCACCAGAAGATGCACACGGAAGAGAAGCGCTACAC GTATAGCCAACTTGGGAAAGATCTTACCCCACCCCCAGATCTTGTCCACCAGGAGGATCTTTCCCTGAGT GAGACCTCCGTTCATTTAGGAGAGAGGTCTACAGATCGGGAGTGTTGTGGAAATAATTTATAAGACAGTC ACTCTGAGTCGGATGGTGCTTCTAAACAGGCATCCTTAGTACATATATGGCTTAGAAGATGCGGATTGTC ACAGTTTTTCAAGTCATTGATAGGCTGTTACTGGTCTGTTGTCTGTCACCAAGGCAGGCAAGCCAAAGGA CTAGACAGCTGATCTTGAGCTGTTCACTGAGGGAGAACCTCTAGAGAATTTTCTAGATTTTATTTGAAGT TACAACACACTCTCAAGTACTTTCAAAGAAGAACTGTATTGTTACTTGAGAAATGGTTCCCTGTCTGAAA GGTTAGAGAAAGGGGCTTTTCTCATGCAAAACTGCAAGTCGGTTCCCTGTTGTCTGGGACTCATCTCTGG CCTTTGCTTCCTCAACTGTTTGATGCCTGGTAATGCAGTTGATGCGATTGCCACCGCATCCTCCAGGCTG CTCCCACTCCTGTCCCAGTGTCTCCCAGTCGCTCCTGGTCATTGTGTATCATTTCTCAGGCGTCATGGCC AAAAGTGCTTCCCTGGTACTGAGCTGGGTTTGTGTCTTGTGCTTCTGTCGCTCAGTGTTCACCACTGCTA CTGTACTTGCTATTAAATACCACAATCATGAACCTTCTCTTTCCTGTTTTGCTGTTTCCCCGAGGACACT AAGGATGTATCATTGGCTCCTATGTCCCTAGTGTCTCGCACATGACAATAAACAAAACAAAAATCCCAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6832277 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6832277 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11471 same embryo
	EMAGE:11470 same embryo
	EMAGE:11468 same embryo
	EMAGE:11469 same embryo
	EMAGE:11473 same embryo
	EurExpress:euxassay_006895 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source