EMAGE mouse gene expression spatial database

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EMAGE:11473

Sbno2 strawberry notch homolog 2 (Drosophila) ( MGI:2448490)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006891. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006891_01	euxassay_006891_02	euxassay_006891_03	euxassay_006891_04

euxassay_006891_05	euxassay_006891_06	euxassay_006891_07	euxassay_006891_08	euxassay_006891_09

euxassay_006891_10	euxassay_006891_11	euxassay_006891_12	euxassay_006891_13	euxassay_006891_14

euxassay_006891_15	euxassay_006891_16	euxassay_006891_17	euxassay_006891_18	euxassay_006891_19

euxassay_006891_20	euxassay_006891_21	euxassay_006891_22	euxassay_006891_23	euxassay_006891_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11473_wholemount_strong.wlz

11473_wholemount_moderate.wlz

11473_wholemount_weak.wlz

11473_wholemount_possible.wlz

11473_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11473_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 11 18 19 20 21

vibrissa

weak

regional

weak expression: see section 07 08 09 21 22 23 24

not examined

regional

not examined expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9210
Entity Detected:	Sbno2, strawberry notch homolog 2 (Drosophila) ( MGI:2448490)
Sequence:	sense strand is shown >T9210 CGGCAACATGGCGCCCAGAACCCGGAAGTGAGCGGCGGCCGCTGAGAGGCTCCGGGAAACCGAGGCGTCG CGGGCTCAGCGATCAGGTCTGAGGATATGACATTACAACTGCCGCTGCCAGCACCACAGAGTAAGCAGTC CAGATAGCCGCCACCCGAGGGTCGCGGAGCGCTGCCTCCACTTGCCTGATGCTGGCAGTGGAACCCACCA TGGATGGGGACTTCCCTCCACATGAGCTCCCACCCCCTGGAGGTGGCATCCAGCTACAGAACCGACTCCT GCACTGTCCTTGGTGGGGCAGTTTCTCACCGTCTTTGTACCCCACCTTCTCCAGTGAAAACCAACAGTTC GTGGGTTCCACCCCATTCCTTGGTGGCCAGTCTTGCCCTGAAACCAGCTACCCCACCACAGCCACCGTCC CCAGCTTCTTCTCCAAGAGCAGCGACTTTCCTCAGGACCCCTCATGCCTCGAGGACCTCTCCAATGCCTC TGTCTTCTCTTCATCTGTGGATTCCCTGTCAGACATCCCAGACACACCTGACTTCCTGCAGGCTGACAGC CTCAACGAGGTGCCTACCATCTGGGATGTCAGCACCACCTCAACTACCCATGACAAGCTGTTCATACCTA GTGGGCCATTCTCGGCTCCTGAAGACCCGGTGACTTCGCTCTCCAGCACTCCACTTCTCATCAGCTATCA GTCACACAGCCAGCCTGAGGAGGAAGAAGGGGAGGAAGAGGAGGAGACTGAGGAACTGGGCCATGCTGAG ACCTATGCTGACTATGTACCATCTAAGTCCAAGATTGGAAAGCAACACCCAGACCGTGTGGTTGAGACCA GCACACTGTCCAGTGTTCCACCCCCGGACATCACCTACACCCTAGCGCTACCCACCTCAGACAACAGCAC CCTGTCTGCCCTACAGCTGGAAGCCATCACCTATGCCTGCCAGCAACACGAGGTCCTGCTGCCCAGTGGG CAGCGTGCTGGTTTTCTGATTGGGGATGGGGCCGGCGTGGGCAAGGGCCGCACAGTGGCTGGCATCATAG TGGAGAACTACCTGCGGGGCCGGAAGAAGGCATTGTGGTTCAGTGCCTCCAATGACCTCAAGTATGATGC GGAACGGGACCTGAGAGACATTGAGGCCCCAGGCATTGCAGTGCATGCATTGAGCAAGATCAAGTACGGT GACAACACTACCTCAGAGGGTGTCCTCTTTGCCACCTACTCTGCCCTGATTGGAGAAAGCCAGGCAGGTG GGCAGCATCGCACACGCCTCCGCCAGATCCTGCAGTGGTGTGGTGAGGGCTTCGATGGTGTGATTGTGTT TGACGAGTGCCACAAAGCCAAGAACGCCAGTTCCACTAAGATGGGCAAGGCCGTGCTGGACCTGCAGAGC AAACTGCCCCAGGCTAGGGTGGTGTACGCCAGTGCCACAGGTGCCTCTGAGCCCCGCAACATGATATACA TGAGCCGGCTGGGCATTTGGGGTGAGGGCACACCCTTCCGGACCTTTGAGGAGTTCCTGCATGCCATTGA GAAGAGGGGCGTGGGTGCCATGGAGATCGTGGCGATGGACATGAAGGTCAGCGGCATGTACATCGCTCGC CAGCTCAGCTTCTCAGGGGTCACCTTCCGAATCGAGGAGATCCCGCTGTCCCCTGCCTTCCAGCAGGTCT ATAATCGGGCAGCCCGGCTGTGGGCCGAGGCCCTGAGCGTGTTCCAGCAGGCAGCTGACTGGATCGGCCT GGAGTCTCGCAAGTCCCTGTGGGGTCAGTTCTGGTCAGCGCACCAGCGCTTCTTTAAGTACCTGTGCATC GCAGCCAAAGTGCACCGGCTAGTGGAGCTGGCACAGCAGGAGCTGAGCAGGGACAAGTGTGTGGTCATCG GGCTGCAGTCCACAGGGGAGGCACGGACTCGAGAGGTGCTGGATGAGAACGAGGGCCGCCTGGACTGCTT CGTCTCCGCAGCAGAAGGTGTCTTCCTGTCCCTGATCCAGAAGCACTTCCCTTCCACCAGGCGGAGGCGT GACCGCGGAGGAGGAAAGAGGAAACGGCGGCCTCGGGGTCGTGGCCCCAAGGCTTCCAGACTGTCACTGG AGGCAGCAGGAGTTATCCGTATTAGTGATGGCAGCAGCACAGAGTCAGATGCTGGCCTGGATAGCGACTT CAATTCATCCCCAGAATCCCTGGTAGACGATGATGTGGTCATTGTAGACGCCCCCACACACCCCACAGAT GACCGAGGTTCCCTGTACCCACTTCAGAGAGACCTGCAGGGCCCTGGTGTCGTGGAACGGGTAGAACGGC TGAAACAGGGTCTGCTTGCCAAGGTGCGAGCGCTGGGCCGGGAGCTGCCAGTCAACACGTTGGACCAGCT CATCCACCAACTTGGGGGTCCTGAGTGTGTGGCCGAGATGACCGGCAGGAAAGGCCGAGTGGTATCGAGG CCTGATGGGACAGTGGTCTTTGAGTCCAGAGCAGAACAGGGCCTGTCCATCGACCATGTGAACCTCAGGG AGAAGCAGCGCTTCATGAGCGGCGAGAAGCTCGTGGCTATTATCTCTGAGGCCTCCAGCTCAGGTGTCTC CCTCCAAGCCGACCGTCGAGTCCAGAACCAGCGGCGCCGGGTACACATGACCCTGGAGCTGCCTTGGAGT GCAGACCGTGCCATTCAACAGTTTGGCCGCACCCACAGGTCCAACCAGGTCTCAGCACCCGAGTACGTCT TCCTTATCTCAGAACTGGCAGGGGAGCGCAGGTTTGCCTCCATTGTGGCCAAGCGGCTCGAAAGTCTGGG TGCCCTCACCCATGGAGATCGCCGCGCCACTGAGTCCCGAGACCTGAGCAAGTACAACTTTGAGAACAAG TATGGCGCCCGTGCGCTCAGCCGCGTCCTTGCTACAATCATGGGCCAGACGGACAACAGGGTGCCCCTGC CCCAGGGCTATCCAGGAGGGGATACCGCCTTCTTTCGGGACATGAAGCAGGGCCTGCTGTCTGTGGGCAT CGGCAGCCGGGAGTCACGCTCGGGCTGCTTGGATGTGGAGAAGGACTGCTCCATCACCAAGTTCCTGAAC CGCATCCTTGGGCTGGAGGTGCACAAGCAGAACGCCCTGTTCCAATACTTCTCCGACACCTTCGACCACC TCATCGAGATCGACAAGAAGGAGGGCAGATACGACATGGGCATCCTAGACCTGGCCCCTGGCATCAACGA GATACATGAAGAAAGCCAGCAGGTGTTCCTGGCACCTGGGCACCCACAGGATGGGCAAGTGGTCTTCTAC AAGCAGATTAGCGTGGACCGCGGTATGAAGTGGGAAGAGGCATTAACTAGGTCGCTGGAGCTCAAAGGTC CCTATGATGGCTTCTACCTCTCCTACAAGGTACGAGGCAGCAAAATGAGCTGCCTCCTGGCAGAACAGAA TCGCGGCGAGTATTTCACCGTGTACAAGCCAAACATCGGCAGACAGAGCCAGCTGGAGACCCTGGACAGC CTGTGCCGGAAGTTTCACCGGGTCACTGTGGAAGAGGCCCGGGAACCCTGGGAGAGCAGCTATGCCCTGT CCTTGGAGCACTGCAGTCACACCACCTGGAACCAGCGCTGCAGACTGACACAGGAGGGCAAGTGCTGCGC CCAGGGCCTGCGCCTCCGCCACCACTACATGCTGTGTGGGGCGCTGCTGCGTGTGTGGGGCCGCATCGCC GCTGTCATGGCGGATGTCAGCAGTAGCAGCTACCTACAGATTGTGCGCCTTAAGACCAAGGACAAGAAGA AGCAAGTGGGCATCAAGATCCCCGAGGGATGCGTTCACCGCGTACTGCAGGAACTGCAGCTGATGGATGC AGAAGTGAAGCGCCGCAGCACCCATGGGTTGGCAGCGCGCCCACCCACTCCACGTGCGATTACGCTTCCT TGCGGTCCGGGTGAGGTGCTGGACTTGACCTACAGTCCCCCAGCTGAGGCTTTCCCAACACCTCCGCGCT TCGCCTTCCCTTCGCTGCCGCCCCCAGACCCCAGCTCTCTGATGCTGGGTGCTAGAGACCCTGCTACCAA CCCTGTGGAGCTGGCACACCAGAGCTGCGACATCAATTTCAGGGAAGTGTTGGAAGACATGCTCCGCTCT TTGCGCGCGGGACCCACCGAAACCCCTGCACCTCTGGTGGGCGTGGGTGGCGGGGGCACAGAGCGACAGA GCGTCATCCACTTCAGCCCACCCTTCCCAAACTCTTAGGCTGCTACTTCAGCCTCTGGATTACAACTGTA CTGGGCAGCAAGGCCTATGGGCTCCTCTGCAAGCAGAGGCTCCCATGACTACTGAGGTGCCTGGCCACAG TGCCTTCCCAGCCAGTGGGGAGTGGGGCCTCTCTGTGCCTCACCTGCTACAGCTGCGCAGCCTAGAAGCC GTGGGGCTTGGTTGTCCCAGAGAGCTGGTAACTCAGGAAATCGGTGCTCGGGCCCCGCTGGCTATGGGGG CTCCACGGGGCAGACCCCGTTAATATATGCAATCCCCTGCTCCCTAAATTATTGTCACCCGCCTCTCCCC AGCCCCGGAGTCTGTGGGCAGCCGGCGGGCCCTGGTTTCTATGTATTTATAATGAACTCGTGTACATATG TAAAGATCTTTTTTAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6831600 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6831600 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11471 same embryo
	EMAGE:11470 same embryo
	EMAGE:11468 same embryo
	EMAGE:11469 same embryo
	EMAGE:11472 same embryo
	EurExpress:euxassay_006891 same experiment
	MGI:4827880 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source