EMAGE mouse gene expression spatial database

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EMAGE:11484

Sprr1a small proline-rich protein 1A ( MGI:106660)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006906. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006906_01	euxassay_006906_02	euxassay_006906_03	euxassay_006906_04

euxassay_006906_05	euxassay_006906_06	euxassay_006906_07	euxassay_006906_08	euxassay_006906_09

euxassay_006906_10	euxassay_006906_11	euxassay_006906_12	euxassay_006906_13	euxassay_006906_14

euxassay_006906_15	euxassay_006906_16	euxassay_006906_17	euxassay_006906_18	euxassay_006906_19

euxassay_006906_20	euxassay_006906_21	euxassay_006906_22	euxassay_006906_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11484_wholemount_strong.wlz

11484_wholemount_moderate.wlz

11484_wholemount_weak.wlz

11484_wholemount_possible.wlz

11484_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11484_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

spotted

strong expression: see section 11 12 14 15

vibrissa

strong

regional

strong expression: see section 02 03 04

anterior naris

strong

regional

strong expression: see section 11 12 13 15 16

external naris

strong

regional

strong expression: see section 11 17

esophagus epithelium

strong

spotted

strong expression: see section 08 moderate expression: see section 09 10 11 12 13

frenulum

strong

regional

strong expression: see section 11 12 14 15

stomach

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 moderate expression: see section 09

stomach fundus

strong

spotted

strong expression: see section 08

bladder

strong

regional

strong expression: see section 11 12 13

kidney pelvis

strong

spotted

strong expression: see section 18 moderate expression: see section 08

ureter

strong

spotted

strong expression: see section 09 17 moderate expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8255
Entity Detected:	Sprr1a, small proline-rich protein 1A ( MGI:106660)
Sequence:	sense strand is shown >T8255 CAACAGAGAACCTGCTCTTCTCTGAGTATTAGGACCAAGTGCTATCTAACCATGAGTTCCCACCAGCAGA AGCAGCCCTGCACTGTACCTCCTCAGCTGCACCAGCAGCAGGTGAAGCAGCCTTGCCAGCCACCACCCCA GGAACCTTGTGCCCCCAAAACCAAGGATCCCTGCCACCCTGTTCCTGAGCCCTGCAACCCCAAGGGGCCA GAGCCCTGCCACCCCAAGGCACCCGAGCCCTGCCACCCCAAGGCACCTGAGCCCTGCAACCCCAAGGTGC CAGAGCCCTGCCAGCCTAAGGTGCCAGAGCCCTGCCAGCCTAAGGTGCCAGAGCCCTGCAACCCCAAGGT GCCAGAGCCCTGCCAACCTAAGGCACCAGAGCCTTGCCACCCCAAGGCGCCTGAGCCCTGCCACCCTGTT GTTCCCGAGCCCTGCCCCTCAACTGTCACTCCATCACCATACCAGCAGAAGACAAAGCAGAAGTAATATT GTCCAGAGCCATGCCTGAAGACCTGATCACCAGATGCTGAGGCTGCTGTCTATCCTGCTTATGAGTCCCA TTGCCTTGTGCTACCAATGCTGTGACCTTCAATCTTAATCCCTCTCTCCTTGCACCACCTAAAAAGTTGA CTCTCATCCTCATCTTCAAGGGCTCCTGAGCCTCTTAACATTGCCCAAAGTCATATTGAATGGCTACACT TTTCATGGCTCAGGATTCATCTGAAGGGGGTGAGGAGTGAGACAAGTGTATGGTCAATATTTTCCCCCCA TTAAATGCCATTTAACTCCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1362548 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1362548 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11485 same embryo
	EMAGE:11486 same embryo
	EMAGE:11487 same embryo
	EurExpress:euxassay_006906 same experiment
	MGI:4828428 same experiment
	EMAGE:31831 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source