EMAGE mouse gene expression spatial database

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EMAGE:11580

Trp53 transformation related protein 53 ( MGI:98834)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003635. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003635_01	euxassay_003635_02	euxassay_003635_03	euxassay_003635_04

euxassay_003635_05	euxassay_003635_06	euxassay_003635_07	euxassay_003635_08	euxassay_003635_09

euxassay_003635_10	euxassay_003635_11	euxassay_003635_12	euxassay_003635_13	euxassay_003635_14

euxassay_003635_15	euxassay_003635_16	euxassay_003635_17	euxassay_003635_18	euxassay_003635_19

euxassay_003635_20	euxassay_003635_21	euxassay_003635_22	euxassay_003635_23	euxassay_003635_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11580_wholemount_strong.wlz

11580_wholemount_moderate.wlz

11580_wholemount_weak.wlz

11580_wholemount_possible.wlz

11580_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11580_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 11 12 13 14

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6641
Entity Detected:	Trp53, transformation related protein 53 ( MGI:98834)
Sequence:	sense strand is shown >T6641 AAGTTCTGTAGCTTCAGTTCATTGGGACCATCCTGGCTGTAGGTAGCGACTACAGTTAGGGGGCACCTAG CATTCAGGCCCTCATCCTCCTCCTTCCCAGCAGGGTGTCACGCTTCTCCGAAGACTGGATGACTGCCATG GAGGAGTCACAGTCGGATATCAGCCTCGAGCTCCCTCTGAGCCAGGAGACATTTTCAGGCTTATGGAAAC TACTTCCTCCAGAAGATATCCTGCCATCACCTCACTGCATGGACGATCTGTTGCTGCCCCAGGATGTTGA GGAGTTTTTTGAAGGCCCAAGTGAAGCCCTCCGAGTGTCAGGAGCTCCTGCAGCACAGGACCCTGTCACC GAGACCCCTGGGCCAGTGGCCCCTGCCCCAGCCACTCCATGGCCCCTGTCATCTTTTGTCCCTTCTCAAA AAACTTACCAGGGCAACTATGGCTTCCACCTGGGCTTCCTGCAGTCTGGGACAGCCAAGTCTGTTATGTG CACGTACTCTCCTCCCCTCAATAAGCTATTCTGCCAGCTGGCGAAGACGTGCCCTGTGCAGTTGTGGGTC AGCGCCACACCTCCAGCTGGGAGCCGTGTCCGCGCCATGGCCATCTACAAGAAGTCACAGCACATGACGG AGGTCGTGAGACGCTGCCCCCACCATGAGCGCTGCTCCGATGGTGATGGCCTGGCTCCTCCCCAGCATCT TATCCGGGTGGAAGGAAATTTGTATCCCGAGTATCTGGAAGACAGGCAGACTTTTCGCCACAGCGTGGTG GTACCTTATGAGCCACCCGAGGCCGGCTCTGAGTATACCACCATCCACTACAAGTACATGTGTAATAGCT CCTGCATGGGGGGCATGAACCGCCGACCTATCCTTACCATCATCACACTGGAAGACTCCAGTGGGAACCT TCTGGGACGGGACAGCTTTGAGGTTCGTGTTTGTGCCTGCCCTGGGAGAGACCGCCGTACAGAAGAAGAA AATTTCCGCAAAAAGGAAGTCCTTTGCCCTGAACTGCCCCCAGGGAGCGCAAAGAGAGCGCTGCCCACCT GCACAAGCGCCTCTCCCCCGCAAAAGAAAAAACCACTTGATGGAGAGTATTTCACCCTCAAGATCCGCGG GCGTAAACGCTTCGAGATGTTCCGGGAGCTGAATGAGGCCTTAGAGTTAAAGGATGCCCATGCTACAGAG GAGTCTGGAGACAGCAGGGCTCACTCCAGCTACCTGAAGACCAAGAAGGGCCAGTCTACTTCCCGCCATA AAAAAACAATGGTCAAGAAAGTGGGGCCTGACTCAGACTGACTGCCTCTGCATCCCGTCCCCATCACCAG CCTCCCCCTCTCCTTGCTGTCTTATGACTTCAGGGCTGAGACACAATCCTCCCGGTCCCTTCTGCTGCCT TTTTTACCTTGTAGCTAGGGCTCAGCCCCCTCTCTGAGTAGTGGTTCCTGGCCCAAGTTGGGGAATAGGT TGATAGTTGTCAGGTCTCTGCTGGCCCAGCGAAATTCTATCCAGCCAGTTGTTGGACCCTGGCACCTACA ATGAAATCTCACCCTACCCCACACCCTGTAAGATTCTATCTTGGGCCCTCATAGGGTCCATATCCTCCAG GGCCTACTTTCCTTCCATTCTGCAAAGCCTGTCTGCATTTATCCACCCCCCACCCTGTCTCCCTCTTTTT TTTTTTTTTACCCCTTTTTATATATCAATTTCCTATTTTACAATAAAATTTTGTTATCACTTAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3599812 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3599812 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11582 same embryo
	EMAGE:11579 same embryo
	EMAGE:11581 same embryo
	EMAGE:11578 same embryo
	EurExpress:euxassay_003635 same experiment
	MGI:4828932 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source