EMAGE mouse gene expression spatial database

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EMAGE:11581

Rbmx RNA binding motif protein, X chromosome ( MGI:1343044)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003647. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003647_01	euxassay_003647_02	euxassay_003647_03	euxassay_003647_04

euxassay_003647_05	euxassay_003647_06	euxassay_003647_07	euxassay_003647_08	euxassay_003647_09

euxassay_003647_10	euxassay_003647_11	euxassay_003647_12	euxassay_003647_13	euxassay_003647_14

euxassay_003647_15	euxassay_003647_16	euxassay_003647_17	euxassay_003647_18	euxassay_003647_19

euxassay_003647_20	euxassay_003647_21	euxassay_003647_22	euxassay_003647_23	euxassay_003647_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11581_wholemount_strong.wlz

11581_wholemount_moderate.wlz

11581_wholemount_weak.wlz

11581_wholemount_possible.wlz

11581_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11581_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6638
Entity Detected:	Rbmx, RNA binding motif protein, X chromosome ( MGI:1343044)
Sequence:	sense strand is shown >T6638 CCCACGCGTCCGGTTCCCGGGTAGTATAAAGATTGCAGTCTCCTTTGTTCGCCCTCGTTGCGCAGTCGTC CTAGCGTCATTTCTGTTCCGTTCTGAAAATCGGTAGCTGTCACTGGTGCGGCGCTGCTCGGGAGGTCCGG TCGGCGAGCTCAGAGGAGCTTGAGACCGTCGCAGTCCCCTCGGACCCCCATCGGTTAAGAGGAAAAATGG TTGAAGCAGATCGCCCAGGAAAGCTCTTTATTGGTGGGCTTAATACAGAGACGAATGAGAAAGCCCTTGA GGCAGTGTTTGGCAAATATGGACGAATAGTGGAAGTTCTTTTGATGAAGGACCGAGAAACGAATAAGTCA AGAGGATTCGCTTTTGTCACTTTTGAAAGCCCAGCAGATGCAAAGGATGCTGCCAGAGACATGAATGGAA AGTCCTTAGATGGGAAAGCCATCAAGGTGGAGCAAGCCACCAAACCATCATTTGAAAGTGGTAGACGTGG ACTACCCCCACCTCCAAGAAGCAGAGGCCCTCCCAGAGGTCTTCGAGGAGGAAGAGGAGGAAGTGGAGGA ACCAGAGGACCCCCCTCCCGGGGAGGACACATGGATGATGGTGGCTACTCCATGAATTTTACCATGAGTT CTTCCAGAGGACCTCTGCCAGTAAAACGAGGACCACCACCACGAAGTGGAGGTCCTCCTCCTAAAAGATC AGCACCTTCTGGACCAGTTCGTAGCAGCAGTGGACTGGGAGGAAGAGCCCCTGTGTCCCGTGGAAGAGAT GGTTACGGAGGCCCGCCACGAAGGGAGCCCCTGCCTTCTCGAAGAGATGTTTATTTGTCCCCGAGAGATG ATGGATATTCCACTAAAGACAGCTATTCAAGCAGAGAATATCCAAGTTCTCGAGATACACGAGATTATGC ACCACCACCAAGAGATTATACTTACCGTGATTATGGTCATTCCAGTTCACGAGATGACTATCCATCAAGA GGCTATAGTGATAGAGATGGCTATGGTCGGGATCGGGACTATTCAGATCATCCAAGTGGAGGTTCCTACA GAGATTCGTATGAGAGTTATGGTAACTCACGTAGTGCTCCACCTACACGAGGGCCCCCGCCATCTTATGG TGGAAGCAGTCGCTATGATGATTACAGCAGCTCACGTGACGGATATGGTGGAAGTCGAGACAGTTACTCA AGCAGCCGAAGTGATCTCTACTCAAGTGGTCGTGATCGCGTGGGCAGACAAGAAAGAGGGCTTCCCCCTT CTATGGAAAGGGGGTACCCTCCTCCACGTGATTCCTACAGCAGTTCAAGCCGCGGAGCACCAAGAGGTGG TGGCCGTGGAGGAAGCCGATCTGATAGAGGGGGAGGCAGAAGCAGATACTAGGAACAAACAAAACTTTTG GACCAAAAAAATCCCCATTCAAAGCAACAAACAAAAGTGGAAACTTTCTGTCATAAACTACCCAAGGACT ACTAAAAGGAAAAATTGTGTTACCTTTTTAAAATTTCCTGTTCCCTTTTAATAATTTTTATGTTCTTGTG AGGGAAAAAGTAAGACGTGTTTAATTTTATTTGCTAGTATGAGCTGCTTTCAACAAGCAAATGGTAGATG TGTGAGCCTTGACTTGTTCCTAGTGTTGTAATTTTCCAAGTGAAAGTGTCCCTGAAGGCCAGCCACTTCC TGATTTTTTTCCCCAGTAAATGAGGCAAGCAACCCCAAGATCATCCACAAACTTCCAGCCATCTAGATGG CCTCTGGTCTCCACTGAGAGCTGAATCGCCCTGCCTGTGCAGACAAGCTAGCCATTAGAGAGGGTTGGTT GGGTGTGCTACTTTCAGTATTTCAGGGTGTCTTCCAACTGAAATCTCAATCTTCGCAGTATATATATTAA AACCAACGAGCAAACCTGAGCTCCGATGCCTAGGATGTACTGTTTACCCAGAAACCCCCCAAAAAAGCAG ATGGATACTGGCCATATTTTGCTTTTTTGACACTTCCTTGGGAATCTACAAGAACCTCCCTTCCCCCTCC CCGAGTAAGACCTAAGACCATTTAAGTGTGTGTTAAACAACTACAAAATACTCAATAAAACGTTTGGCCA AAGTCAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3599678 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3599678 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11582 same embryo
	EMAGE:11580 same embryo
	EMAGE:11579 same embryo
	EMAGE:11578 same embryo
	EurExpress:euxassay_003647 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source